Estabelecimento e desenvolvimento &lt;i&gt;in vitro&lt;/i&gt; de &lt;i&gt;Eugenia involucrata&lt;/i&gt; DC.: influência do tipo de explante e do meio nutritivo

Golle, Diego Pascoal; Reiniger, Lia Rejane Silveira; Curti, Aline Ritter; León, Enrique Asterio Benítez

doi:10.5902/198050985092

Cited by 21 publications

(23 citation statements)

References 2 publications

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“…Phenol oxidation, which is associated with the dark coloring of the explant, is another impediment to be overcome during the establishment of ligneous plants in vitro. According to Golle et al (2012), the presence of oxidation in the explants, though undesirable, do not appear to hinder the establishment and development of the cultures in vitro. This was not confirmed in the present study because the oxidation compromised E. pyriformis micropropagation in a way that did not allow us to evaluate the development of the plants in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…Phenols and other exudates in in vitro cultivation systems can compromise the micropropagation process, since they inhibit the initial growth of the explants, which may lead to its death (BASSAN et al, 2006). The concentration of such compounds is related to the type of explant used, with nodal segments usually presenting lower concentrations than do apical segments (GOLLE et al, 2012), which justifies the addition of antioxidant substances to the culture media.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

Assis¹,

Rodrigues²,

Pasqual³

et al. 2018

Biosci. J.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

Assis¹,

Rodrigues²,

Pasqual³

et al. 2018

Biosci. J.

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“…It has been demonstrated that different MS salt concentrations influence growth in several species (Assis et al 2012;Martins et al 2015;Shekhawat et al 2015;Singh et al 2015). Generally, lower salt concentrations stimulate rooting (Sorace et al 2008;Golle et al 2012;Shekhawat et al 2015). Fargoso Monfort et al (2018) found that in Ocimum basilicum volatile constituents diminished with higher salt concentrations.…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

434

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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“…Esta fruteira é considerada por muitos autores, como de difícil propagação, uma vez que ao usar as técnicas assexuadas de estaquia (LOUROSA et al, 2011), alporquia (HOSSEL et al, 2014, cultivo in vitro (GOLLE et al, 2012;PAIM, 2011) DANNER et al (2011) e HOSSEL et al (2013 como eficiente em sementes deoutra recalcitrante, a jabuticabeira (Plinia sp. ).Esta técnica promove a retirada de oxigênio do ambiente das sementes, reduzindo a respiração e o metabolismo das mesmas, além de diminuir as trocas gasosas com o ambiente, aumentando-se os níveis de gás carbônico e reduzindo-se do oxigênio, o que mantém assim a viabilidade por maior período.…”

Section: Introductionunclassified

Hidrocondicionamento e atmosfera modificada ou controlada para a conservação da viabilidade de sementes de cerejeira-da-mata

Hossel

Alegretti

et al. 2016

Revista Brasileira De Tecnologia Aplicada Nas Ciências Agrárias

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ResumoO objetivo do trabalho foi avaliar o efeito do hidrocondicionamento e de técnicas de atmosfera controlada e modificada na conservação da viabilidade de sementes armazenadas de cerejeira-do-mato (E. involucrata) por diferentes períodos. O experimento 1 foi instalado com delineamento experimental deblocos ao acaso, em fatorial 2 x 4 x 2 (pré-hidrocondicionamento x técnica de armazenamento x tempo de armazenamento), com 4 repetições, de 50 sementes por parcela. As sementes extraídas foram separadas em dois lotes, sendo um submetido ao pré-hidrocondicionamento em água destilada durante 24 horas e outro não. Sementes hidrocondicionadas ou não foram submetidas a quatro técnicas de armazenamento, sendo estes, embalamento a vácuo, revestimento com biofilme de fécula de mandioca (3% m/v), vácuo + biofilme de fécula de mandioca e sem tratamento (testemunha). Posteriormente, as sementes foram mantidas em câmara fria, em temperatura de 5ºC durante 5 e 30 dias. O experimento 2 foi instalado em delineamento inteiramente casualizado, em esquema fatorial 2 x 3 (ambiente x período de armazenamento), com quatro repetições de 50 sementes por parcela. Foram testadas duas condições de armazenamento, sendo estas de ambiente natural e controlado sob temperatura de 6° C, permanecendo as sementes nestas condições por 3 períodos (15, 30 e 45 dias).Para o armazenamento das sementes de cerejeira-da-mata deve-se utilizar técnicas a vácuo isolada ou com revestimento de biofilme. Ocorre perda de viabilidade das sementes quando o teor de água foi reduzido a 39,84% ou se mantidas em condição ambiente por mais de 30 dias. Palavras chave: Eugenia involucrata DC.; Myrtaceae; Propagação sexuada; Armazenamento. Hydropriming and modified atmosphere or controlled to conserve viability cerejada-mata seeds AbstractThe objective was to evaluate the effect of hydropriming and conservation techniques (vacuum and biofilm) of stored seeds of cereja-da-mata (E. involucrata) for different periods. Experiment 1 was installed with experimental design of randomized blocks in a factorial 2 x 4 x 2 (pre-storage x technique hydropriming x storage time), with four replications of 50 seeds each. The extracted seeds were separated into two batches, one being subjected to the pre-hydropriming in distilled water for 24 hours and the other not. Hidrocondicionadas seeds or have not undergone four storage techniques, which are, vacuum packaging, coating cassava starch biofilm (3% w/v), vacuum + starch biofilm cassava and untreated (control). Then the seeds were kept in cold storage, in 5°C temperature for 5 and 30 days. Experiment 2 was completely randomized in a factorial 2 x 3 (environment x storage period), with four replications of 50 seeds per plot. Two conditions have tested these natural environment and under controlled temperature 6°C, the remaining seeds under these conditions for 3 times (15, 30 and 45 days). For storage of cereja-da-mata seeds should be used techniques isolated vacuum or biofilm coating. There is loss of seed viability when the water conten...

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Estabelecimento e desenvolvimento <i>in vitro</i> de <i>Eugenia involucrata</i> DC.: influência do tipo de explante e do meio nutritivo

Cited by 21 publications

References 2 publications

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

In vitro plant tissue culture: means for production of biological active compounds

Hidrocondicionamento e atmosfera modificada ou controlada para a conservação da viabilidade de sementes de cerejeira-da-mata

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