Establishment and adipocyte differentiation of polycystic ovary syndrome‐derived induced pluripotent stem cells

Yang, Sheng; Ding, Sheng; Jiang, Xianglong; Sun, Bolan; Xu, Qianhua

doi:10.1111/cpr.12258

Cited by 11 publications

(12 citation statements)

References 28 publications

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“…Other studies report contradictory results [18][19][20][21][22][23] suggesting that AA pushed cells to differentiate into adipogenesis or neurogenesis. However, either cell status and/or the protocols used to promote cell differentiation were different to those used here.…”

Section: Aa and Es Cell Differentiationmentioning

confidence: 98%

“…However, either cell status and/or the protocols used to promote cell differentiation were different to those used here. Other studies used already-committed cells, VM embryonic cells [18], cells from PCOS patients [21], embryonic carcinoma P19 cells [23] or different ES R1 lines [19], and added different molecules in the culture media, from ␤FGF [18] or complex cocktail [20] to activinA+BMP4 [22]. As ES cell differentiation appears dependent on context, level of commitment, added molecules, differentiation protocol, and other factors, we opted to use totipotent cells here in a protocol allowing spontaneous cell differentiation without added molecules in culture media.…”

Section: Aa and Es Cell Differentiationmentioning

confidence: 99%

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Ascorbic acid drives the differentiation of mesoderm‐derived embryonic stem cells. Involvement of p38 MAPK/CREB and SVCT2 transporter

Rahman

Bordignon

Culerrier

et al. 2017

Molecular Nutrition Food Res

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The study demonstrated that AA drives mesoderm-derived stem cell differentiation toward myogenesis and osteogenesis and also inhibits adipogenesis. Further experiments found that AA competes with retinoic acid (RA) to drive cell differentiation in a dose-dependent manner: AA inhibited neurogenic differentiation and stimulated myogenesis whereas RA did the reverse. The AA-dependent differentiation of embryonic stem cells was shown to involve a p38 MAPK/CREB pathway, probably stimulated by cAMP via adenylate cyclases. In addition, SVCT2, the intracellular transporter of AA, acted as a receptor. Finally, we showed that activation/repression of specific differentiation markers is associated with epigenetic changes in their associated promoters. We discuss the impact of these findings in terms of obesity and aging.

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Section: Aa and Es Cell Differentiationmentioning

confidence: 98%

Section: Aa and Es Cell Differentiationmentioning

confidence: 99%

Ascorbic acid drives the differentiation of mesoderm‐derived embryonic stem cells. Involvement of p38 MAPK/CREB and SVCT2 transporter

Rahman

Bordignon

Culerrier

et al. 2017

Molecular Nutrition Food Res

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“…The adult cells were collected and reprogrammed from the POI patients with a familial inheritance as described previously [13, 14]. In short, the iPSCs were grown on irradiated mouse embryonic fibroblasts (MEFs) in N 2 B 27 medium (Invitrogen) supplemented with 100 U/ml LIF (ESGRO Millipore), 3 mM GSK3 inhibitor CHIR99021 (Axon Medchem BV), SB203580 (TOCRIS bioscience), 5 mM SP600125 (TOCRIS bioscience), and 1 mM MEK inhibitor PD0325901 (Stemgent).…”

Section: Methodsmentioning

confidence: 99%

“…The detailed protocol for stem cell culture and PGC differentiation is mainly based on paper described by Yang et al [13]. The main procedure is included pre-induction and PGC induction.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, a recent study showed that Sox17 regulated the fate of human PGCs; nevertheless, BLIMP1 repressed the expression of endodermal and other somatic genes during the differentiation of human PGCs. The molecular mechanism differences between mouse and human PGC specification may lead to their divergent embryonic development and pluripotent states, which might affect other early cell-fate decisions [13]. This result also offered us insight into understanding the basis of the progression of pluripotent stem cells to PGCs.…”

Section: Introductionmentioning

confidence: 99%

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Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells

Yang

Ding

et al. 2019

Stem Cell Res Ther

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Background Premature ovarian insufficiency (POI) is a common disease in reproductive women. The pathogenesis of POI is not clear, although it is known that it involves the disorder of oocyte differentiation and development. The introduction of reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) offers a unique opportunity to study many aspects of POI from cell differentiation in vitro that could ultimately lead to novel drug development and testing to help treat the disorder. Methods The fibroblasts from POI patients, including fragile X syndrome, abnormal karyotype (45, X; 45, X/46, XX; 45, XO and 47, XXX), and the gene mutation ( FIGLA and GDF9 ) were reprogrammed to pluripotency status by retroviral transduction using defined factors. The morphology, growth characteristics, gene expression profiles, epigenetic status, and in vitro and in vivo differentiation potential of the POI-1-iPSCs (from fragile X syndrome) were analyzed. Then, POI-1-iPSCs were induced to differentiation into primordial germ cells (PGCs) with DNA methyltransferase inhibitors. Results The iPSCs were successfully generated from POI patients’ fibroblasts. The formed iPS clones have the same characteristics of human ESCs. POI-1-iPSCs were successfully generated with germline competence. The POI-1-iPSCs, with genotypes of fragile X syndrome, can be induced to differentiation into PGCs with high efficiency under our culture system by DNA demethylation. This study proved that disease-specific iPSC lines derived from POI patients could be generated and successfully differentiated into PGCs. Conclusions We established some novel, systemic cell models for the studying of the pathogenesis of POI patients. Second, DNA demethylation may accelerate the induction of human PGCs from iPSCs in vitro and the conclusion needs further exploration. This represents an important step in the novel approach for the study of the pathophysiology and potential egg resource for POI patients. Electronic supplementary material The online version of this article (10.1186/s13287-019-1261-6) contains supplementary material, which is available to authorized users.

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Regenerative Medicine Perspectives in Polycystic Ovary Syndrome

Arjmand

Alaei

Heravani

et al. 2021

Advances in Experimental Medicine and Biology

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Establishment and adipocyte differentiation of polycystic ovary syndrome‐derived induced pluripotent stem cells

Abstract: This protocol provides a new biological cell model and approach for studying pathogenesis of PCOS and discovering potential drugs to treat it.

Cited by 11 publications

References 28 publications

Ascorbic acid drives the differentiation of mesoderm‐derived embryonic stem cells. Involvement of p38 MAPK/CREB and SVCT2 transporter

Ascorbic acid drives the differentiation of mesoderm‐derived embryonic stem cells. Involvement of p38 MAPK/CREB and SVCT2 transporter

Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells

Regenerative Medicine Perspectives in Polycystic Ovary Syndrome

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