Establishment of an In Vitro System Representing the Chicken Gut-Associated Lymphoid Tissue

Abstract

The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a+, a majority of those emigrating onto the supporting membrane were Bu-1a− and IgM+. Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined.

The effects of different velogenic NDV infections on the chicken bursa of Fabricius

“…Avian bursa of Fabricius had been identified to play an important role in the development of B lymphocytes as studies showed that the surgical removal of this organ during the embryonic stage of a bird compromised its antibody production thus leading to immunosuppresion [ 11 , 12 ]. The organ serves as an essential site for generation of antibody diversity by gene conversion.…”