Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir.

Khalafalla, Mutasim M.; Daffalla, Hussien M.; Abdellatef, Eltayb; Agabna, Elsadig; El‐Shemy, Hany A.

doi:10.1631/jzus.b1000205

Cited by 13 publications

(12 citation statements)

References 27 publications

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“…In agreement, Prathanturarug et al (2012) found that IBA was superior to NAA in terms of root induction percentage and number of roots per shoot with S. hutanguriana regenerated shoots after 8 weeks in culture. A similar trend was also observed for other species including Centella asiatica (Tiwari et al 2000), Boscia senegalensis (Khalafalla et al 2011), Pappea capensis (Mngomba et al 2007) and Teucrium fruticans (Frabetti et al 2009). …”

Section: Discussionsupporting

confidence: 79%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.

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Section: Discussionsupporting

confidence: 79%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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“…These results seems to be in complies with those found by Faisal et al (2006) when they evaluate the effect of BA, kin and 2-iP as supplements to Murashige and Skoog (MS) medium since they found that benzyladinine (BA) at an optimal concentration (5.0 μM) was effective in inducing multiple shoots. Also, Khalafalla et al (2011) on Boscia senegalensis achieved the highest number of shoots per explant on MS medium supplemented with 3 mg l -1 BA, while the highest shoot length was obtained with 1 mg l -1 BA.…”

Section: Establishment Stagementioning

confidence: 91%

Micropropagation of Yucca Plant by Using Guar and Locust Bean Seed Powder as an Alternative Cheap Gelling Agent

Hassan

Moubarak

2020

Scient. J. of Flowers and Ornament. Plants

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The present study aims to determine the best type and concentration of growth regulators and find cheap and/or readilyavailable alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other tested cytokinins concerning yucca shoot multiplication and growth parameters [number of shoots/explant, number of leaves/shoot, and shoot length (cm)] which recorded 4.75, 14.00 and 6.50 cm, respectively. Moreover, the combination between 2 g l-1 agar and 6 g l-1 guar as gelling agents produced the maximum shoot growth values. During rooting stage, data clear that 1.0 mg l-1 NAA increased number of leaves/shoot, number of roots/shoot and root length with mean values of 20.50, 3.75 and 5.22 cm, respectively. Finally, rooted plantlets were successfully acclimatized with maximum survivability percentage (100%) in soil mixture of sand and peat moss (1:1. v/v).

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“…Another successful result has been obtained by Khalafalla et al (2011). They found that the maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium.…”

Section: The Interaction Effects Between Ba Concentrations and Subculmentioning

confidence: 93%

Use of Biotechnology for Multiplication of Curcuma longa L. plant during six subcultures

Koriesh¹

2018

HJSC

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The aim of this study was to set a protocol for the in vitro culture of Curcuma longa L. Sprouted bud explants of C. longa L. were explanted on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1 during six subcultures. The maximum number of multiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l −1 BA at 4 th and 5 th subculture with (90 and 100% multiplication frequency) respectively. The optimum BA concentrations for maximum number of multiplication varied across subcultures. The 7 mg l-1 BA concentration at the late subculture 4 th , 5 th and 6 th produce highest values of (number of shoots, longest shoot, number of leaves and fresh weight).The maximum number of roots (6.38 roots explant-1) was induced from 3 mg l −1 IBA. The tallest roots (40.6 and 36.5 cm) were obtained on MS medium supplemented with 1 mg l −1 NAA and 3 mg l −1 IBA. Also, the heaviest fresh weight was obtained from medium containing 2 and 3 mg l −1 IBA and all concentrations of NAA. In vitro plantlets immediately acclimatized to greenhouse conditions, showing 100% survival rates in a peat moss and vermiculite (1:3) medium.

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Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir.

Cited by 13 publications

References 27 publications

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Micropropagation of Yucca Plant by Using Guar and Locust Bean Seed Powder as an Alternative Cheap Gelling Agent

Use of Biotechnology for Multiplication of Curcuma longa L. plant during six subcultures

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