Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis

Lee, Seung Tae; Choi, M. Y.; Lee, Eun Ju; Gong, Seung Pyo; Jang, Mi; Park, Sang Hyun; Jee, Hyang; Kim, Dae Yong; Han, Jae Yong; Lim, Jeong Mook

doi:10.1016/j.fertnstert.2007.01.099

Cited by 36 publications

(55 citation statements)

References 15 publications

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“…This preference for a testicular location reflects the different histogenesis of testicular and ovarian germ cell tumors, the former originating from primitive germ cells with a malignant character, 3 while the latter mostly have a parthenogenetic origin from postmeiotic or meiotic cells. 4,5 For these reasons, the presence of EC in the ovary is extremely rare. In 40 years' experience of ovarian germ cell tumor consultations, the senior author of this article has only identified 3 bona fide cases.…”

Section: Antibody (D) Cdx2 Stains Epithelial Cells Focally (E) Villmentioning

confidence: 99%

“…The latter have, however, a different histogenesis, originating in the testis from malignant germ cells, 3 as opposed to ovarian germ cell neoplasms, which are mostly parthenogenetically conditioned. 4,5 Consequently, MTGCTs exhibit genetic markers, such as a 12p isochromosome and chromosome 12 overrepresentation, 6 that are less frequently observed in MOGCTs, 7 which often resemble testicular infantile teratomas/yolk sac tumours, 8 with the pure ovarian teratomas showing negativity for 12p. 9 For this reason, although equating ovarian and testicular germ cell tumors may not be totally correct biologically, their morphology and diagnostic immunohistochemistry are practically similar.…”

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confidence: 99%

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Germ Cell Tumors of the Ovary: An Update

Nogales

Dulcey

Preda

2014

Archives of Pathology &Amp; Laboratory Medicine

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Context.—The field of ovarian germ cell tumors (OGCTs) has remained relatively unchanged in the last 2 decades. However, the introduction of new stem cell pluripotency markers has provided a new understanding into the identification and taxonomy of OGCT types. New data have provided new insights into unusual teratoma-associated autoimmune disorders and the origin of gliomatosis peritonei. Objective.—To review the impact of new pluripotency markers in the diagnosis of malignant OGCT (MOGCT) and analyze new nomenclature proposals and clinicopathologic entities. Data Sources.—Ovarian germ cell tumors from routine material and expert consultation files at San Cecilio University Hospital, Granada, Spain, and the relevant literature were reviewed. Conclusions.—Although a correct diagnosis of MOGCT can often be made with histologic and classic immunohistochemical studies, the new immunohistochemical pluripotency markers give higher diagnostic accuracy. Germ cell tumors represent a caricature of the phases of normal embryonic differentiation from primordial germ and stem cells to extraembryonal and somatic tissue differentiation. Since every stage of differentiation and its related tumor type exhibit characteristic markers, the analysis of their expression facilitates tumor typing, thus complementing the use of classic antibodies. They also allow a more precise evaluation of the degree of immaturity in teratoma. The new term, primitive endodermal tumors, simplifies the understanding of the complex histology of the yolk sac tumor group, as this terminology encompasses its multiple endodermal differentiations. Recently described autoimmune encephalitis due to antibodies against the N-methyl-d-aspartate receptor has become the most frequent autoimmune disorder associated with ovarian teratoma.

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Section: Antibody (D) Cdx2 Stains Epithelial Cells Focally (E) Villmentioning

confidence: 99%

mentioning

confidence: 99%

Germ Cell Tumors of the Ovary: An Update

Nogales

Dulcey

Preda

2014

Archives of Pathology &Amp; Laboratory Medicine

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“…In recent studies, α-minimal essential medium and Dulbecco's modified Eagle's medium were used as basal media for the in vitro growth of preantral follicles [35,42,48,[53][54][55]. Both media were supplemented with various culture additives, including serum, growth factors, gonadotropins, antibiotics and substrates.…”

Section: Protocol For In Vitro Growth Of Preantral Folliclesmentioning

confidence: 99%

“…Then, their ovaries are removed aseptically, placed in Leibovitz's L-15 medium at 37 C, cut into pieces using a 30-gauge needle and rinsed several times with the collection medium. The preantral follicles are retrieved using an adjustable pipet under an inverted microscope, washed three times in droplets of Leibovitz's L-15 medium, separated and cultured singly in 20-μl droplets [42,48,49]. …”

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confidence: 99%

Derivation of Histocompatible Stem Cells from Ovarian Tissue

2010

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Abstract. Somatic cell nuclear transfer, the first established technique for producing patient-specific autologous stem cells, inevitably requires the sacrifice of viable embryos. To circumvent the serious ethical issues associated with this use of embryos, researchers have developed several alternative methods for the production of histocompatible stem cells. In our research, we have used two methods to derive histocompatible stem cells from murine ovarian tissue. First, we have established autologous stem cells by culturing degeneration-fated preantral follicles to produce developmentally competent, mature oocytes and then parthenogenetically activating these mature oocytes to acquire genetic homogeneity. Second, we have used cell-to-cell interactions to derive stem cells from ovarian stromal cells without undertaking genetic modification. We have successfully derived autologous murine stem cells by manipulating primary and early secondary follicles in vitro, and this method has proved successful even for follicles retrieved from aged ovaries. Furthermore, we believe that it will be possible to isolate stem cells directly from nongermline ovarian tissue or to derive stem cells by culturing the ovarian cells with other somatic cells. If achieved, these aims will greatly advance the development of induced pluripotent stem cell technology, as well as tissue-specific stem cell research. In this review, we introduce the relevant technologies for establishing histocompatible stem cells from ovarian tissue cells without undertaking genetic manipulation and review the current limitations of, and future research directions in, stem cell biology. Key words: Autologous, Ovarian cells, Parthenogenesis, Preantral follicle, Stem cell (J. Reprod. Dev. 56: [481][482][483][484][485][486][487][488][489][490][491][492][493][494] 2010) luripotent stem cells are widely recognized as critical to the success of certain cutting-edge medical technologies, such as cell replacement, organ regeneration, tissue engineering, and rejuvenation therapy [1][2][3]. However, the potential clinical practicability of stem-cell-based techniques is limited by the absolute requirement for patient-stem cell histocompatibility or patient acquisition of immune tolerance [4]. Another obstacle to the use of stem cells is the current lack of effective techniques for regulating cell-to-cell interactions and for inducing target differentiation of stem cells. The future success of tissue regeneration therapies will also depend on the optimization of stem-cell transplantation techniques and the development of strategies for inducing transplanted stem cells to resume their normal functions. In addition, all stem cell-based procedures must be carried out in an ethically acceptable manner.Efforts to develop stem-cell-based techniques such as those described above have been extensive. In our research, we have consistently attempted to develop novel methodologies for the establishment of histocompatible stem cells. At a very early stage in stem cell research, a ...

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“…Mice parthenogenetic embryonic stem cell lines have been well established (Lee et al, 2008;Ju et al, 2008); however, the potential development of parthenogenetic embryos and the pESC derivation efficiency is still very low (Liu et al, 1998;Cibelli et al, 2002). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo (produced by the optimized method) and the identified male embryo, aimed to investigate a method to improve the development of parthenogenetic embryos and pESC derivation in mice.…”

Section: Discussionmentioning

confidence: 99%

Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation

Qiu¹,

Nan²,

Xiao³

et al. 2017

Turk J Biol

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Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher blastulation rate than the haploid type (P < 0.05). The treatment of strontium chloride (SrCl 2 ) combined with cytochalasin B for 4 h produced the highest heterozygous diploid rate and blastulation rate. Our self-made concave hole system was used for the aggregation of the parthenogenetic heterozygous diploid embryo with the male embryo identified by the duplex PCR method, and we found that the chimeric embryo had an improved rate of blastulation and pESC isolation.

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Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis

Cited by 36 publications

References 15 publications

Germ Cell Tumors of the Ovary: An Update

Germ Cell Tumors of the Ovary: An Update

Derivation of Histocompatible Stem Cells from Ovarian Tissue

Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation

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