Establishment of long-term embryogenic cultures of seedless Vitis vinifera cultivars — a synergistic effect of auxins and the role of abscisic acid

Preservation of endangered Tunisian grapevine cultivars using embryogenic cultures

“…Similar results on the preservation of pro-embryogenic cultures from four seedless grapevine cultivars on MS supplemented with 2,4-D and IASP during 10 months reported, however, a spontaneous embryogenesis and premature germination (Perl et al 1995). These authors inhibited these phenomena by adding ABA (abscissic acid) and maintained their embryogenic calluses for more than 18 months.…”

Somatic Embryogenesis in Grapevine

“…For example, the type of solidifying agent has shown to significantly influence callus initiation and formation of embryogenic structures, with new gelling polymers being superior to agar (Perl et al, 1995;Torregrosa, 1998).…”

“…Addition of exogenous abscissic acid (ABA) to the medium often inhibited somatic embryo germination (Rajasekaran et al, 1982;Takeno et aI., 1983;Gray, 1989;Perl et al, 1995); however, it has been signalled that this treatment can prevent precocious germination and slightly promote normal regeneration into plants (Goebel-Tourand et aI., 1993;Faure et al, 1998).…”

Regeneration of grape (Vitis labruscana cv. Kyoho) by shoot-tip culture

“…Because both auxins and cytokinins can affect adventitious embryogenesis from cultured grape explants (Reustle et al, 1994;Perl et al, 1995, Torregrosa et al, 1995Nakano et al, 1997), we investigated their effects on callus induction in MS media. In our stem explant cultures, calli were induced and developed regardless of the kind of auxin present in the medium, whereas the potential for callus induction in our leaf cultures depended on a specific auxin Figure 6.…”