Establishment of transgenic mice carrying gene encoding human zinc finger protein 191

Abstract

AIM:Human zinc finger protein 191 (ZNF191) was cloned and characterized as a Krüppel-like transcription factor, which might be relevant to many diseases such as liver cancer, neuropsychiatric and cardiovascular diseases. Although progress has been made recently, the biological function of ZNF191 remains largely unidentified. The aim of this study was to establish a ZNF 191 transgenic mouse model, which would promote the functional study of ZNF191. METHODS:Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. The offsprings were identified by PCR and Southern blot analysis. ZNF 191 gene expression was analyzed by RT-PCR. Transgenic founder mice were used to establish transgenic mouse lineages. The first generation (F1) and the second generation (F2) mice were identified by PCR analysis. Ten-week transgenic mice were used for pathological examination.

Zinc Finger Protein 191 (ZNF191/Zfp191) Is Necessary to Maintain Neural Cells As Cycling Progenitors

“…The knock out of Zfp191 induces embryonic lethality as early as E7.5 [6], which makes this approach unsuitable for the analysis of ZNF191 function in the developing CNS. On the other hand, mice carrying a transgene that induces misexpression of the human zinc finger protein 191 gene have been generated with no consequences observed on the different analyzed tissues, suggesting that compensatory mechanisms may take place at early stages of development to counteract ZNF191 misexpression [7]. Therefore, to avoid these pitfalls and to further characterize ZNF191 function by overexpression experiments, we carried out electroporation experiments in the chick embryo in ovo .…”

“…Interestingly, transgenic misexpression of ZNF38 in mice did not result in any phenotypic abnormality but showed the occurrence of a slight overproliferation of these progenitors that was dependent on the copy number of the transgene [54]. Similarly, the same approach with ZNF191 did not result in any remarkable phenotype; however, the number of transgene copies and the level of ZNF191 expression were not reported in this case [7].…”

“…On the basis of expression data, ZNF191 was supposed to play a role during hematopoiesis [5], but precise information on the biological function of ZNF/Zfp191 is still lacking. In the mouse, this factor is required during early developmental stages, because mutant embryos do not survive beyond embryonic day (E) 7.5 without any clear cause of lethality [6], whereas its misexpression does not result in any remarkable phenotype [7].…”

Zinc Finger Protein 24 is a Prognostic Factor in Ovarian Serous Carcinoma

“…The physiological functions of ZNF24 can be partially reflected by its knock-in and knock-out mice. Transgenic mice demonstrated that ZNF24 knock-in did not cause obvious pathobiological alterations in transgenic mice 14. However, knock-out of ZFP191, the mouse homolog of human ZNF24, results in severe retardation of development and embryos die at ∼ 7.5 days postfertilization 15.…”

“…Transgenic mice demonstrated that ZNF24 knock-in did not cause obvious pathobiological alterations in transgenic mice. 14 However, knock-out of ZFP191, the mouse homolog of human ZNF24, results in severe retardation of development and embryos die at ∼ 7.5 days postfertilization. 15 Therefore, ZNF24 plays indispensable roles in early embryonic development such as central nervous system development.…”