Estimating microcystin levels at recreational sites in western Lake Erie and Ohio

Francy, Donna S.; Brady, Amie M.G.; Ecker, Christopher D.; Graham, Jennifer L.; Stelzer, Erin A.; Struffolino, Pamela; Dwyer, Daryl F.; Loftin, Keith A.

doi:10.1016/j.hal.2016.07.003

Cited by 47 publications

(30 citation statements)

References 33 publications

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“…The extracellular/total WLW ratios for these three samples ranged from 22% -28%, with an average of 24%. Bastien, et al [36] previously reported ranges from 1% -100% with an average of 23%. Application of conservative criteria for detection limits using hand-held fluorometers resulted in a limited database from which to conduct statistical analysis using extracellular PC.…”

Section: Extracellular Photopigment and Toxin Analysismentioning

confidence: 91%

Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin

Leland¹,

Haney²

2018

JWARP

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The management of cyanobacteria and potential exposure to associated biotoxins requires the allocation of scarce resources across a range of freshwater resources within various jurisdictions. Cost effective and reliable methods for sample processing and analysis form the foundation of the protocol yielding reliable data from which to derive important decisions. In this study the utilization of new methods to collect, process and analyze samples enhanced our ability to evaluate cyanobacterial populations. Extraction of phycocyanin using the single freeze thaw method provided more accurate and precise measurements (CV 4.7% and 6.4%), offering a simple and cost-effective means to overcome the influence of morphological variability. In-vacuo concentration of samples prior to ELISA analysis provided a detection limit of 0.001 µg•L ). These methods and sampling protocol could be used in other aquatic systems across a broader regional landscape to estimate the levels of microcystins.

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Section: Extracellular Photopigment and Toxin Analysismentioning

confidence: 91%

Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin

Leland¹,

Haney²

2018

JWARP

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“…The additional importance of the moving 5-day average of the east-west wind component is likely a result of westerlies concentrating buoyant cyanobacteria on the eastern shore close to where the WISPstation is positioned. Similarially in Lake Erie, the wind component, also with a 5-day lag, was found to be significant in predicting microcystin concentration which was highest following a period of onshore wind and lower during periods of offshore wind [72]. The NPMR model was developed for a limited time-period (April-October 2018).…”

Section: Intra and Inter-daily Variation Of Chl-a Concentrations Frommentioning

confidence: 92%

The Use of Multisource Optical Sensors to Study Phytoplankton Spatio-Temporal Variation in a Shallow Turbid Lake

Bresciani

Pinardi

Free

et al. 2020

Water

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Lake water quality monitoring has the potential to be improved through integrating detailed spatial information from new generation remote sensing satellites with high frequency observations from in situ optical sensors (WISPstation). We applied this approach for Lake Trasimeno with the aim of increasing knowledge of phytoplankton dynamics at different temporal and spatial scales. High frequency chlorophyll-a data from the WISPstation was modeled using non-parametric multiplicative regression. The ‘day of year’ was the most important factor, reflecting the seasonal progression of a phytoplankton bloom from July to September. In addition, weather factors such as the east–west wind component were also significant in predicting phytoplankton seasonal and diurnal patterns. Sentinel 3-OLCI and Sentinel 2-MSI satellites delivered 42 images in 2018 that successfully mapped the spatial and seasonal change in chlorophyll-a. The potential influence of localized inflows in contributing to increased chlorophyll-a in mid-summer was visualized. The satellite data also allowed an estimation of quality status at a much finer scale than traditional manual methods. Good correspondence was found with manually collected field data but more significantly, the greatly increased spatial and temporal resolution provided by satellite and WISPstation sensors clearly offers an unprecedented resource in the research and management of aquatic resources.

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“…To reduce the risks posed by MCs, the World Health Organization (WHO) has established a provisional guideline of 1 μg L −1 MCs in water destined for use as human drinking water, and 20 μg L −1 for recreational waters . MCs, comprised of a family of potent hepatotoxins, are the cyanotoxins with the most frequent occurrence and they show the greatest resistance to degradation because of their stable cyclic peptide structure.…”

Section: Introductionmentioning

confidence: 99%

“…MCs are a large group of cyclic heptapeptides, with more than 100 structural variants. MC‐LR contains Leucine amino acid and an arginine amino acid in variable positions, and it is the most common and toxic variant .…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

et al. 2018

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Microcystins are potent hepatotoxins produced by cyanobacteria, which proliferate in wastewaters with high nutrient content. Due to their high toxicity and potential risk to human health, even at low concentrations, the development of a sensitive and rapid method for the monitoring of microcystin‐LR (MC‐LR) in water samples is of great importance. In this context, a new direct electrochemical nano‐immunosensor for MC‐LR detection using the liquid crystal (E)‐1‐decyl‐4‐[(4‐decyloxyphenyl)diazenyl]pyridinium bromide (Br‐Py) as a redox probe and gold nanoparticles stabilized in bovine serum albumin (AuNP‐BSA) is described herein. The microcystin‐LR antibody (anti‐MC‐LR) was covalently immobilized using N‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimide hydrochloride (EDC) and N‐hydroxysuccinimide (NHS) on an AuNP‐BSA/BrPy film. The proposed sensor response is based on the inhibition of the Br‐Py electrochemical signal after the specific interaction of MC‐LR with immobilized anti‐MC‐LR on the electrode surface. The electrochemical behavior of the immunosensor was studied by square‐wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, using SWV and an incubation time of 15 min, the immunosensor exhibits a linear response to MC‐LR concentrations of 0.05 to 500.0 ng mL−1 with a detection limit of 0.05 ng mL−1. The anti‐MC‐LR/AuNP‐BSA/Br‐Py/GCE was successfully applied in the determination of MC‐LR in spiked seawater samples.

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Estimating microcystin levels at recreational sites in western Lake Erie and Ohio

Cited by 47 publications

References 33 publications

Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin

Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin

The Use of Multisource Optical Sensors to Study Phytoplankton Spatio-Temporal Variation in a Shallow Turbid Lake

Direct Electrochemical Nano‐immunosensor for Microcystin‐LR in Seawater

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