“…Although controlling specimen's temperature is technically feasible, measuring unfrozen water content is much more challenging. Several methods and techniques have been developed to evaluate the unfrozen water content at negative temperature, including dilatometry [42,43], gas dilatometry [44], adiabatic calorimetry [45,46], isothermal calorimetry [28], differential scanning calorimetry [10,47,48], X-ray diffraction [49,50], time/frequency domain reflectometry (TDR/FDR) [51][52][53] and pulsed nuclear magnetic resonance (P-NMR) [38,54,55]. Among these methods, TDR and P-NMR are the two most common ones.…”