ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [<i>AZADIRACHTA INDICA A. JUSS</i>] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Deota, Pradeep T.; Upadhyay, Piyush R.; Patel, Kirti; Mehta, K. J.; Kamath, B. V.; Mehta, Manisha

doi:10.1081/jlc-100100484

Cited by 14 publications

(15 citation statements)

References 15 publications

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“…Azadirachtin has been isolated and quantified in several reports from the seeds of the tree (Deota et al, ; Jarvis & Morgan, ; Kumar et al, ; S. M. Lee, Olsen, Schweizer, & Klocke, ). Nimbidinin and nimbidic acid were also isolated from extract of seed kernels (Mitra, Garg, & Pandey, ).…”

Section: Phytochemistrymentioning

confidence: 99%

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A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica

Saleem

Muhammad

Hussain

et al. 2018

Phytotherapy Research

128

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Azadirachta indica L. is a multipurpose medicinal tree of family Meliaceae. It occurs in tropical and semitropical regions of the world. Different parts of this miraculous tree are used to treat pyrexia, headache, ulcer, respiratory disorders, cancer, diabetes, leprosy, malaria, dengue, chicken pox, and dermal complications. The tree is popular for its pharmacological attributes such as hypolipidemic, antifertility, microbicidal, antidiabetic, anti-inflammatory, hepatoprotective, antipyretic, hypoglycemic, insecticidal, nematicidal, antiulcer, antioxidant, neuroprotective, cardioprotective, and antileishmaniasis properties. A. indica is also rich in various phytochemicals for pharmaceuticals such as alkaloids, steroids, flavonoids, terpenoids, fatty acids, and carbohydrates. The fungicidal potential of the tree is due to the presence of azadirachtin and nimbin. Herein, we have compiled a comprehensive review of phytochemical profile, pharmacological attributes, and therapeutic prospective of this multipurpose tree.

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Section: Phytochemistrymentioning

confidence: 99%

“…Azadirachtin has been isolated and quantified in several reports from the seeds of the tree (Deota et al, 2000;Jarvis & Morgan, 2000;Kumar et al, 1996; S. M. Lee, Olsen, Schweizer, & Klocke, 1988).…”

mentioning

confidence: 99%

A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica

Saleem

Muhammad

Hussain

et al. 2018

Phytotherapy Research

128

View full text Add to dashboard Cite

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“…A multi-step sample preparation method following the methodology of Schroder and Nakanishi [31] has been reported by Deota et al [32]. The method reported by Schaaf et al [33] based on HPLC-Mass Spectrophotometry (MS) is sensitive enough to detect the azadirachtin in ng mL -1 , but is not suitable for routine analysis since HPLC-MS is not available in all laboratories.…”

Section: Introductionmentioning

confidence: 97%

Determination of azadirachtin and fatty acid methyl esters of Azadirachta indica seeds by HPLC and GLC

Kaushik¹

2002

Anal Bioanal Chem

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A simple and economical method has been developed to estimate the azadirachtin content and fatty acid composition of neem kernels. Neem kernels are crushed and soaked overnight in ethanol. The extract obtained is analysed by HPLC after filtering through a 0.22 micro m membrane. The peaks are separated using acetonitrile-water (40:60) 1 mL min(-1) as the mobile phase on an RP-18 column and monitored at 214 nm. For the determination of fatty acid composition, the fatty acids are directly transmethylated in the kernel powder by heating with methanol-acetyl chloride-benzene (20:1:4, v/v) for 1 h in a water bath. The fatty acid methyl esters (FAMEs) obtained are extracted in hexane and analysed using GLC. The separation of the FAMEs is achieved using an RH-Wax column using temperature programming, 170-200 degrees C at 2 degrees min(-1). The peaks are detected using an FID. Both the methods do not require any clean up or defatting of seeds. This results in faster, easier and more economical sample preparation.

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“…Silica gel, phenyl or C18 can be used as the stationary phase. A chromatographic system as employed by Deota et al [21] gave the best result. Azadirachtin with high purity and recovery can be obtained using starting material with low azadirachtin content.…”

Section: Comparison Of Chromatographic Purification Of Azadirachtinmentioning

confidence: 96%

“…In most combinations, high purity (>70%) azadirachtin can be obtained after the application of HPLC method as the final step. [14][15][16][17][18][19][20][21] A simple method to produce a mixture of neem limonoids or limonoid powder has been developed by Melwita et al [22] Further investigation is required to develop limonoid powders as the potential starting material for neem-based product. In this work, limonoid powder was employed as the starting material for column chromatography purification of azadirachtin.…”

Section: Introductionmentioning

confidence: 99%

Purification of Azadirachtin via Silica Gel Column Chromatography

Melwita

Tsigie

Ismadji

et al. 2011

Journal of Liquid Chromatography & Related Technologies

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ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [AZADIRACHTA INDICA A. JUSS] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Cited by 14 publications

References 15 publications

A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica

A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica

Determination of azadirachtin and fatty acid methyl esters of Azadirachta indica seeds by HPLC and GLC

Purification of Azadirachtin via Silica Gel Column Chromatography

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