Estimation of amlodipine besylate, valsartan and hydrochlorothiazide in bulk mixture and tablet by UV spectrophotometry

Abstract

A simple, precise, accurate and economic simultaneous UV spectrophotometric method has been developed for the estimation of amlodipine besylate, valsartan and hydrochlorothiazide in combination in bulk mixture and tablet. The estimation was based upon measurement of absorbance at absorbance maxima of 359 nm, 317 nm and 250 nm for amlodipine besylate, hydrochlorothiazide and valsartan in methanol, respectively in bulk mixture and tablet. The Beer Lambert's law obeyed in the concentration range 5-25 μg/ml, 10-50 μg/ml and 5-25 μg/ml for amlodipine besylate, hydrochlorothiazide and valsartan, respectively. The estimation of bulk mixture and tablet was carried out by simultaneous equation, Q-analysis and area under curve method for estimation of amlodipine besylate and hydrochlorothiazide and standard curve method for estimation of valsartan. The results were found to be in the range of 99.6±1.52% to 102±0.51%. Method was validated with respect to specificity, linearity, range, accuracy, precision, LOD, LOQ, robustness, ruggedness and can be applied for routine analysis of tablet dosage forms.

Quinoline Antimalarials Increase the Antibacterial Activity of Ampicillin

“…In this study, quinine showed detectable antibacterial activity against the three organisms used in the study with an MIC of 1,000 µg/mL against E. coli ATCC 25922, S. aureus NCTC 6571, and E. coli LLH029E. The MIC falls within the reported range of values (Kharal et al, 2009;Abreu et al, 2014). Our data show that quinine is bactericidal at 1,000 µg/mL and while this concentration is unlikely to be achieved physiologically during treatment, the additivity uncovered in this work indicates that lower concentrations may produce therapeutic effects in combination with antibacterials.…”

“…Of more interest is the fact that chloroquine and quinine have been reported to have some antibacterial activity, albeit at high concentrations, arising from their structural similarities to (Wolf et al, 2002;Lv et al, 2007;Davidson et al, 2008;Kharal et al, 2009;Bawa et al, 2010;Achan et al, 2011;Jagadeesh et al, 2014) quinolone antibacterials.…”

"Comparative Analytic Study by UV Spectrophotometric, Structural Characterization through Fourier Transformed Infrared Spectroscopy (FTIR) Method and Disintegration Analysis for Different Brands of Amlodipine Tablets Available in Malaysia"

“…Amlodipine will selectively block the Ca2+ ion influx across the cell membrane. Thus, amlodipine will exert direct actions and effects on vascular smooth muscle [1][2][3][4].…”

Quality Control Measurement and in Vitro Bioequivalence of Valsartan and Atenolol Tablets Marketed in Ukraine

“…Therefore, analytical methods for their separation and quantification in pharmaceutical formulations and inhuman plasma are needed for quality control and therapeutic drug monitoring, respectively. Several techniques have been reported in the literature for determination of valsartan individually and combination with other drug other than atenolol [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] in pharmaceutical dosage forms or human serum samples. Atenolol (Fig.…”