Estimation of cardiomyocyte transmembrane potential with potential-sensitive fluorescent probes

Abstract

Transmembrane potentials on the plasma (ADOp) and mitochondrial (ADOrn) membrane of isolated rat cardiomyocytes were estimated using the potential-sensitive fluorescent probe DSM. The values were -93+4 and-196+11 mV, respectively. SuFan significantly decreased the reduction of ADO m induced by chemical hypoxia. The effects of antiarrhythmic drugs on changes in Ad~ were studied using the fluorescent probe dis-C3-(5 ). Lidocaine, novo-P cainamide, richlocalne, and leocaine blocked depolarization of the myocyte plasma membrane induced by electrical stimulation and did not affect the AdPm.

2-phenyl-1-(3-pyrrolidin-1-il-propyl)-1H-indole hydrochloride (SS-68): Antiarrhythmic and Cardioprotective Activity and Its Molecular Mechanisms of Action (Part II)

“…The isolation of cardiomyocytes from the left ventricle of the white non-linear white male rats, the procedure of loading cells with Fura-2/AM and SBFI probes, the registration of fluorescence were carried out according to the methods described by A. I. Khankoeva et al (1997Khankoeva et al ( , 1998. The effect of SS-68 on the calcium conductivity of the I f /HCN channels of the ventricular cardiomyocytes was studied in experiments on white non-linear male rats.…”

“…Cardiomyocytes were isolated from the left ventricle of the heart, cells were loaded with a Fura2/AM probe, and fluorescence was recorded according to the method described by A.I. Khankoeva et al (1997Khankoeva et al ( , 1998.…”