Estimation of progesterone in buffalo milk by radioimmunoassay

Karir, T.; Nagvekar, U. H.; Samuel, Grace; Sivaprasad, N.

doi:10.1007/s10967-006-0045-5

Cited by 8 publications

(4 citation statements)

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“…Phenol is a highly activated aromatic moiety towards electrophilic substitution with iodine radioisotopes suitable for radiodiagnostics ( 123 I, 124 I) or radiotherapy ( 131 I) (Figure 2). Here, for experimentation, we radiolabeled the synthesized thermoresponsive poly (N-isopropylacrylamide)-Ltyrosinamide with 125 I by electrophilic substitution using by Chloramine-T 36,37 and used the product for evaluation in animal tumor model.…”

Section: Characterization Of the Pnipam-l-tyrosinamide Polymermentioning

confidence: 99%

Preparation and evaluation of radioiodinated thermoresponsive polymer based on poly(N‐isopropyl acrylamide) for radiotherapy

Karir¹,

Sarma

Samuel³

et al. 2013

J of Applied Polymer Sci

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A thermoresponsive polymer based on poly N-isopropylacrylamide (PNIPAM) was synthesized and radioiodinated to explore its potential use in localized radiotherapy. The synthesized PNIPAM polymer was functionalized with L-tyrosinamide to facilitate radioiodination. The content of tyrosinamide groups in the polymer was assayed spectrophotometrically (k ¼ 275nm). The functionalized polymer showed a cloud point temperature of 29-31 C and phase separation at 35 o C, as revealed by Differential Scanning Calorimetry (DSC) and Dynamic Light Scattering (DLS). The phase transition temperature is conducive for preferential localization of the polymer at the site of injection due to changes in the polymer conformation at body temperature. For in vivo demonstration, the biodistribution studies of radioiodinated polymer were carried out in Swiss mice bearing fibrosarcoma tumor. Biodistribution studies showed a retention of 30% of the injected labeled polymer, PNIPAM-125 I-tyrosinamide, in the solid tumor tissues after 2 h of intratumoral injection. Although the activity decreased with time, 3-4% of the injected dose (i.d) was found to be retained in the tumor on 5 d post injection. The results suggest the potential use of thermosensitive polymer based on poly N-isopropylacrylamide for locoregional radionuclide therapy.

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Section: Characterization Of the Pnipam-l-tyrosinamide Polymermentioning

confidence: 99%

Preparation and evaluation of radioiodinated thermoresponsive polymer based on poly(N‐isopropyl acrylamide) for radiotherapy

Karir¹,

Sarma

Samuel³

et al. 2013

J of Applied Polymer Sci

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“…For this purpose, the concept of timed AI must be followed which is only possible with the accurate and timely detection of heat. Inefficient and inaccurate heat detection results in failure of conception during that attempt, which in turn leads to significant economic losses (Karir et al, 2006) [2] . The efficient and accurate detection of estrus and the timing of AI has remained major challenge to improve reproductive and economic efficiencies of dairy farms (Heersche and nebel, 1994) [3] .…”

Section: Introductionmentioning

confidence: 99%

“…One of the modern methods of estrus detection may be Infrared thermography, which has successfully been used for various purposes in various farm and wild animals. Infrared thermography (IRT) is a temperature measurement tool based on the ability of all objects to emit characteristic infrared radiation as a function of their temperature (Kastberger and Stachl, 2003) [4] . This radiation is proportional to the surface temperature of the body (Bitar et al, 2009) [5] , and is evaluated using a thermographic camera, which produces images with different color patterns depending on the temperature of the imaged objects.…”

Section: Introductionmentioning

confidence: 99%

Infrared thermographical differentiation of estrus and non-estrus stages of dairy animals

Tiwari¹,

Singh²,

Sirohi³

et al. 2021

Pharma Innovation

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The present investigation was planned to evaluate the usefulness of Infrared thermorgraphical camera for discrimination of various stages of estrus i.e. onset of estrus, standing heat and non estrus conditions in sahiwal cows. The experiments were conducted on ten healthy, cyclic sahiwal cows were maintained at DDD farm of LFC at DUVASU, Mathura. In present study the mean infrared thermographical temperature of muzzle and vulva of sahiwal cows during standing heat stage (34.72±0.21 and 39.15±0.15 ºC, respectively) were found to be significantly (p<0.01) high as compared to two other stages of estrus. Thus, from present study it was concluded that infrared thermoradiography may be used as the efficient tool for detection of estrus and its different stages in sahiwal cows.

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“…In fact, progesterone is currently used for pregnancy diagnosis in cows and it has been reported that its concentration decreases rapidly after calving. Moreover, some authors have also reported higher concentrations of progesterone in bovine milk than in blood plasma, despite following plasmatic variations 20, 21…”

Section: Introductionmentioning

confidence: 99%

Determination of naturally occurring progestogens in bovine milk as their oxime derivatives using high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Regal

Nebot

Vázquez

et al. 2010

J. Sci. Food Agric.

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Estimation of progesterone in buffalo milk by radioimmunoassay

Cited by 8 publications

References 0 publications

Preparation and evaluation of radioiodinated thermoresponsive polymer based on poly(N‐isopropyl acrylamide) for radiotherapy

Preparation and evaluation of radioiodinated thermoresponsive polymer based on poly(N‐isopropyl acrylamide) for radiotherapy

Infrared thermographical differentiation of estrus and non-estrus stages of dairy animals

Determination of naturally occurring progestogens in bovine milk as their oxime derivatives using high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

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