Estimation of serum malondialdehyde in potentially malignant disorders and post-antioxidant treated patients: A biochemical study

Dsouza, Deepa; Bg, Subhas; Shetty, Shishir Ram; Balan, Preethi

doi:10.4103/0976-237x.107438

Cited by 28 publications

(19 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Control participants without the habit of tobacco consumption have shown the least peroxidation activity as compared with the controls with tobacco habits. A similar result was reported by Chole et al .,[ 19 ] D'souza et al .,[ 20 ] and Subapriya et al . [ 10 ] ( P < 0.05) in serum samples; whereas Khanna et al .…”

Section: Discussionmentioning

confidence: 99%

Analysis of plasma lipid peroxidation and antioxidant enzymes status in patients of oral leukoplakia: A case control study

Srivastava

Shrivastava

2016

J Int Soc Prevent Communit Dent

View full text Add to dashboard Cite

Aims and Objectives:Imbalances between the oxidant-antioxidant status have been implicated in the pathogenesis of several diseases, including oral cancer. Mostly, all oral cancer lesions are preceded by a stage of premalignancy. The present study aims to evaluate lipid peroxidation and antioxidant status in the venous blood of patients with different clinicopathologic stages of leukoplakia.Materials and Methods:A case control study was designed with the inclusion of 20 new cases of histopathologically proven leukoplakia of various clinical stages along with an equal number of positive and negative control individuals. The concentrations of thiobarbituric acid reactive substances and the activities of the antioxidant enzymes, namely superoxide dismutase, reduced glutathione, glutathione peroxidase, and catalase, were estimated in plasma using spectrophotometric methods. The data are expressed as mean ± SD. The statistical comparisons between and within the study groups were performed by one-way analysis of variance followed by post hoc analysis. Karl Pearson correlation was performed for the biochemical parameters within the group and between the groups. For statistically significant correlations, simple linear regression was performed.Results:Significant enhanced lipid peroxidation (P < 0.001) with a decrease in antioxidants (P < 0.001) was observed in the venous blood of leukoplakia patients compared with positive as well as negative controls. Accordingly, significant (P < 0.001) pattern of progression in thiobarbituric acid reactive substances levels was observed at various clinical stages among patients of both control groups. Among enzymes, glutathione showed significant (P < 0.001) reduction along the stages on comparison with two control groups.Conclusion:Enhanced lipid peroxidation and compromised antioxidant defense in plasma indicate the development of oxidative stress. Among the antioxidant enzymes, reduced glutathione and glutathione Pperoxidase may play a decisive role in malignant transformation.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of plasma lipid peroxidation and antioxidant enzymes status in patients of oral leukoplakia: A case control study

Srivastava

Shrivastava

2016

J Int Soc Prevent Communit Dent

View full text Add to dashboard Cite

show abstract

“…The extracted liver was cleaned and homogenized in chilled buffered saline, the aliquot so obtained was used for the estimation of CAT [58], SOD [59], and MDA [60].…”

Section: Estimation Of Catalase (Cat) Superoxide Dismutase (Sod) Andmentioning

confidence: 99%

Crocus sativus L. Extract Containing Polyphenols Modulates Oxidative Stress and Inflammatory Response against Anti-Tuberculosis Drugs-Induced Liver Injury

Wali

Pillai

Dhaheri

et al. 2020

Plants

View full text Add to dashboard Cite

The purpose of this study is to analyze the polyphenolic rich extract of Crocus sativus L. petals (CSP) in modulating liver oxidative stress and inflammatory response status against rifampicin isoniazid (INH-RIF) drug-induced liver injury. The INH-RIF was administered for 14 days with varying doses in Wistar rats, while silymarin was administered as standard dose. We report the defensive impacts of CSP against INH-RIF induced liver oxidative stress and proinflammatory cytokine. The CSP treatment at both doses significantly controlled all modulating biochemical hepatic injury indicators and resulted in the attenuation of arbitral INH-RIF damage. The components present in CSP identified by LC–ESI-Q-TOF–MS were found to be flavonoids and fatty acids. It can be inferred that CSP possesses a hepatoprotective capacity against INH-RIF-mediated hepatic injury, which may prove to be a medically beneficial natural product for the management of drug-induced liver injury.

show abstract

“…The MDA calorimetric assay provides a sensitive tool for detection of the MDA, by a Humalyzer junior photometer (Human Diagnostics, Germany) at the Biochemistry Department, University of Alexandria, Egypt. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate MDA-TBA adduct that can be easily quantified colorimetrically(OD = 532 nm) [8] .…”

Section: Ii-biochemical Study 1-malondialdehyde (Mda)mentioning

confidence: 99%

Histological study of the effect of zinc oxide nanoparticles on the cardiomyocytes of adult male albino rats with reference to the role of mitochondria.

zoheir¹,

medwar²,

Solaiman³

et al. 2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

Back ground: Zinc oxide nanoparticles (ZnO-NPs) are among the most commonly used metal nanoparticles and are nowadays increasingly used in food. This might increase the chances of their exposure to the human body via the oral route. The heart is an important toxic site for nanoparticles. Aim: The aim of the current study was to investigate the possible histological changes that might accompany the oral administration of two different doses of ZnO-NPs on left ventricular cardiomyocytes of adult albino rats. Materials and Methods: Forty-eight adult male rats were included. The animals were randomly divided into 3 main groups. Group I (Control group). Group II (ZnO-NPs group) was further subdivided randomly into subgroup IIa that were given ZnO-NPs at a dose of 100mg/kg body weight/day. Subgroup IIb received 400 mg/kg body weight/day ZnO-NPs for 28 days. Group III (Withdrawal group), the animals of this group did not receive any treatment for four weeks after the 28 days of treatment. Specimens from the heart apex were processed for microscopic examination. Serum level of oxidative stress and cardiac makers, as well as the morphometric study (for collagen quantification and mitochondrial morphometry), were done. Results: The light and the electron microscopes revealed histological changes in the left ventricular cardiomyocytes. Changes were dose-dependent. Group II revealed some pale stained muscle fibers with pyknotic nuclei. Others showed deeply acidophilic sarcoplasm. The electron microscope examination confirmed these changes and showed focal myolysis and abnormal mitochondria. This was associated with some hyperchromatic nuclei. After withdrawal histological changes were exaggerated. Serum marker of oxidative stress and cardiac markers showed a significant increase. Mitochondrial morphometry demonstrated fragmentation. Conclusion: The study demonstrated that oral ZnO-NPs are associated with variable microscopic changes, which is dosedependent. These changes didn't improve after the withdrawal of the ZnO-NPs administration.

show abstract

Estimation of serum malondialdehyde in potentially malignant disorders and post-antioxidant treated patients: A biochemical study

Cited by 28 publications

References 11 publications

Analysis of plasma lipid peroxidation and antioxidant enzymes status in patients of oral leukoplakia: A case control study

Analysis of plasma lipid peroxidation and antioxidant enzymes status in patients of oral leukoplakia: A case control study

Crocus sativus L. Extract Containing Polyphenols Modulates Oxidative Stress and Inflammatory Response against Anti-Tuberculosis Drugs-Induced Liver Injury

Histological study of the effect of zinc oxide nanoparticles on the cardiomyocytes of adult male albino rats with reference to the role of mitochondria.

Contact Info

Product

Resources

About