Estimation of the sensitivity of four sampling methods for Mycoplasma hyopneumoniae detection in live pigs using a Bayesian approach

Fablet, C.; Marois, C.; Kobisch, Marylène; Madec, F.; Rose, Nicolas

doi:10.1016/j.vetmic.2009.12.001

Cited by 79 publications

(66 citation statements)

References 21 publications

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“…This confirms previous reports (Kurth and others 2002, Sibila and others 2004a, 2007, Marois and others 2007, Fablet and others 2010) and is consistent with the fact that not the nose, but the trachea and bronchi are the main multiplication sites of M hyopneumoniae (Blanchard and others 1992). In total, 90 pigs were restrained five times for collecting blood and BAL fluid, tracheo-bronchial and nasal swabs samples.…”

Section: Discussionsupporting

confidence: 93%

“…Nasal swabs, tracheo-bronchial swabs (Marois and others 2007, Fablet and others 2010) and BAL fluids (Villarreal and others 2011b) were collected from approximately 30 randomly selected animals per group that were serially sampled at different ages (Table 1). Nasal swabs (Copan, Italy) were collected at weaning (21 days), halfway through the nursery period (49 days), at the end of the nursery period (74 days), halfway through the fattening period (136 days) and prior to slaughter (200 days).…”

Section: Methodsmentioning

confidence: 99%

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Efficacy of early Mycoplasma hyopneumoniae vaccination against mixed respiratory disease in older fattening pigs

et al. 2014

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The present field study investigated the efficacy of early Mycoplasma hyopneumoniae vaccination in a farrow-to-finish pig herd with respiratory disease late in the fattening period due to combined infections with M hyopneumoniae and viral pathogens. Five hundred and forty piglets were randomly divided into three groups of 180 piglets each: two groups were vaccinated (Stellamune Once) at either 7 (V1) or 21 days of age (V2), and a third group was left non-vaccinated (NV). The three treatment groups were housed in different pens within the same compartment during the nursery period, and were housed in different but identical compartments during the fattening period. The efficacy was evaluated using performance and pneumonia lesions. The average daily weight gain during the fattening period was 19 (V1) and 18 g/day (V2) higher in both vaccinated groups when compared with the NV group. However, the difference was not statistically significant (P>0.05). The prevalence of pneumonia was significantly lower in both vaccinated groups (V1: 71.5 and V2: 67.1 per cent) when compared with the NV group (80.2 per cent) (P<0.05). There were no significant differences between the two vaccination groups. In conclusion, in the present herd with respiratory disease during the second half of the fattening period caused by M hyopneumoniae and viral infections, prevalence of pneumonia lesions were significantly reduced and growth losses numerically (not statistically significant) decreased by both vaccination schedules.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Efficacy of early Mycoplasma hyopneumoniae vaccination against mixed respiratory disease in older fattening pigs

et al. 2014

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“…It is also possible that due to coincidence a lower number of positive animals was detected than the animals that were really infected with M. hyopneumoniae, although the samples were taken at random. Nasal swabs were collected, as it is safer for the researchers and less stressful for the animals than taking tracheal swabs (Thacker, 2004;Fablet et al, 2010). However, nasal swabs are less sensitive to detect DNA of M. hyopneumoniae than tracheal swab or BALf.…”

Section: Tablementioning

confidence: 99%

Impact of particulate matter and ammonia on average daily weight gain, mortality and lung lesions in pigs

Michiels

Piepers

Ulens³

et al. 2015

Preventive Veterinary Medicine

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“…The method of nasal sampling used in this study was previously described by Fablet et al (2010) with some modifications. Both nasal cavities of each pig were swabbed with sterilized swabs that were inserted into the nostrils by rotation to reach deep into the turbinates.…”

Section: Nasal Swab Collectionmentioning

confidence: 99%

Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets

Hua¹,

Wu²,

Bai³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The Jiangquhai porcine lean strain (JQHPL) is a new pork meat-type strain that has been developed in recent years from the parent lines Duroc, Fengjing, and Jiangquhai pigs (DurocxFengjing pigxJiangquhai pig). Enzootic pneumonia (EP) in pigs induced by Mycoplasma hyopneumoniae (M. hyopneumoniae) is a chronic respiratory disease of pigs, generating high economic losses in the swine industry. Here, we investigated the degree of resistance to M. hyopneumoniae for the Jiangquhai porcine lean strain and the Duroc positive farrowing barn. When the oldest suckling piglets were 37 days old, nasal swabs were collected from all the piglets (ranging from 4 to 37 days old) to detect the M. hyopneumoniae pathogen using n-PCR and M. hyopneumoniae specific SIgA using ELISA. Positive M. hyopneumoniae infection rates in both the strains increased with age; however, positive rates for JQHPL were lower compared to DLY at 14 to 35 days old. The level of the specific SIgA rose rapidly in JQHPL respiratory tracts, particularly in piglets 21 to 35 days in age compared to DLY piglets of the same age; however, the level of the specific SIgA in DLY also marginally increased. In conclusion, JQHPL pigs exhibits higher resistance to M. hyopneumoniae compared to DLY. It is possible that this characteristic is caused by the faster and stronger mucosal immunity phenotype of the JQHPL strain.

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Estimation of the sensitivity of four sampling methods for Mycoplasma hyopneumoniae detection in live pigs using a Bayesian approach

Cited by 79 publications

References 21 publications

Efficacy of early Mycoplasma hyopneumoniae vaccination against mixed respiratory disease in older fattening pigs

Efficacy of early Mycoplasma hyopneumoniae vaccination against mixed respiratory disease in older fattening pigs

Impact of particulate matter and ammonia on average daily weight gain, mortality and lung lesions in pigs

Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets

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