Estimation of Total Flavonoids Content (Tfc) and Anti Oxidant Activities of Methanolic Whole Plant Extract of Biophytum Sensitivum Linn

Kalita, Pratap; Tapan, Barman K; Pal, Tapas; Kalita, Ramen

doi:10.22270/jddt.v3i4.546

Cited by 121 publications

(107 citation statements)

References 4 publications

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“…The total flavonoid assay was run as descibed by Kalita et al (2013) using quercetin as the standard at 415 nm. The total flavonoid content was expressed in mg equivalents of quercetin/g dry leaf units (mg QE/g dw.…”

Section: Total Flavonoid Contentmentioning

confidence: 99%

Antioxidant properties and detailed polyphenol profiling of European hornbeam (Carpinus betulus L.) leaves by multiple antioxidant capacity assays and high-performance liquid chromatography/multistage electrospray mass spectrometry

Hofmann

Nebehaj

Albert

2016

Industrial Crops and Products

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a b s t r a c tAccording to recent studies, the antioxidant and anticancer related properties of European hornbeam (Carpinus betulus L.) leaf extracts are remarkable, making it a possible raw material for medicine, requiring the identification of major active compounds and the assessment of the antioxidant properties of the extracts. In the present work the high-performance liquid chromatographic/multistage mass spectrometric characterization of the antioxidant polyphenolic compounds as well as the evaluation of the seasonal changes in the major antioxidant capacity and related parameters (DPPH, ABTS, FRAP, total phenol content, total flavonoid content and total flavan-3-ol content) have been carried out for the leaves of European hornbeam for the first time. The extracts exhibited the highest antioxidant capacity in August, basing on the FRAP (106.24 ± 3.10 mg AAE/g dw.) and DPPH (IC 50 : 4.63 ± 0.88 g/ml) assays and in May, based on the ABTS (329.78 ± 23.89 mg TE/g dw.) method. From the August extract a total of 171 compounds, including phenolic acids, flavonoid glycosides, tannins, catechins and procyanidins have been characterized and identified. According to the HPLC-PDA peak areas, the most abundant polyphenolic compounds in the August extracts were chlorogenic acid, ellagic acid, ellagitannins, myricetin-, luteolin-, quercetin-and apigenin glycosides. Further research is needed for the elucidation of the compounds which are primarily responsible for the antioxidant properties.

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Section: Total Flavonoid Contentmentioning

confidence: 99%

Antioxidant properties and detailed polyphenol profiling of European hornbeam (Carpinus betulus L.) leaves by multiple antioxidant capacity assays and high-performance liquid chromatography/multistage electrospray mass spectrometry

Hofmann

Nebehaj

Albert

2016

Industrial Crops and Products

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“…The total phenolic content was expressed as milligrams of tannic acid equivalents per gram of dry weight (mg TAE/g DW) of the plant, using a standard plot of Tannic acid. 14 …”

Section: Total Phenolic Contentmentioning

confidence: 99%

“…This might be due to the presence of strong biologically active components such as phenols and polyphenols, etc., which show remarkable activity against free radicals. 20 Lower the IC 50 value higher will be the antioxidant free radical scavenging ability. When compared with BHA, the plant showed better IC 50 value and this can be potentially used as a natural antioxidant.…”

Section: Dpph Free Radical Scavenging Assaymentioning

confidence: 99%

Preliminary Phytochemical analysis and In vitro Antioxidant, FTIR Spectroscopy, Anti-diabetic activity of Acacia catechu ethanolic seed extract

Lakshmi¹,

Ramasamy²,

Thirumalaikumaran³

2015

Phcog J

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Objective: To evaluate the preliminary phytochemical analysis and in vitro antioxidant activity, anti-diabetic effect of ethanolic seed extract of Acacia catechu against the alpha amylase and alpha glucosidase digestive enzymes in the pancreas and small intestine. Methods: Preliminary phytochemical analysis was done by adopting the method of Evans. Antioxidant assay is performed by DPPH, ABTS and FRAP assay, Anti diabetic activity was determined by modified method of miller, the extract at different concentrations was tested for mammalian alpha amylase and alpha glucosidase enzyme inhibitory assay under the controlled experimental conditions and subjected to determination of absorbance. Results: The present study reveals the presence of few secondary metabolites and the extract exhibits potent Antioxidant activity and a concentration dependent inhibition of Alpha amylase and Alpha glucosidase. Conclusion: From the present study it can be concluded that ethanolic seed extract of Acacia catechu possessed marked in vitro antioxidant and anti-diabetic effect. The effect was plausibly due to the presence of phenolic contents of Acacia catechu.

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“…The absorbance of the samples was measured at 510 nm. By using a quercetin standard the concentration of flavanoids in the extracts was determined [7].…”

Section: Determination the Total Level Of Flavonoid (Aluminum Chloridmentioning

confidence: 99%

Floral Extracts of Allamanda Blanchetii and Allamanda Cathartica Are Comparatively Higher Resource of Anti-Oxidants and Polysaccharides Than Leaf and Stem Extracts

Ghosh

Banerjee

2018

Int J Curr Pharm Sci

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Objective: The present study undertakes a comparative analysis of the level of secondary metabolites present in the leaf, flower and stem of the two ornamental plants, Allamanda blanchetii and Allamanda cathartica. Methods:The two plant species, Allamanda blanchetii and Allamanda cathartica were collected, washed, shade dried in room temperature and powered in mechanical grinder. Phytochemicals were extracted from the power with methanol and double distilled water. The estimation of flavonoids, polyphenols, polysaccharide were done by standard methods and the anti-oxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) discoloration assay.Results: Our study reveals that the flower of both species contain highest amount of secondary metabolites in crude methanolic and aqueous extracts. In case of leaf, the methanolic extracts contain higher amount of polyphenol, flavonoid and anti-oxidant property in comparison to aqueous extracts, where as the aqueous extract contain higher amount of polysaccharide content than its counterpart. In stem, crude organic extract has higher amount of polyphenol and flavonoid and the aqueous extract has higher amount of polysaccharide and anti-oxidant property. Conclusion:The flower of Allamanda cathartica and Allamanda blanchetii has higher amount of flavonoids, polyphenols, polysaccharide and the floral extracts display comparatively higher anti-oxidant property.

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Estimation of Total Flavonoids Content (Tfc) and Anti Oxidant Activities of Methanolic Whole Plant Extract of Biophytum Sensitivum Linn

Cited by 121 publications

References 4 publications

Antioxidant properties and detailed polyphenol profiling of European hornbeam (Carpinus betulus L.) leaves by multiple antioxidant capacity assays and high-performance liquid chromatography/multistage electrospray mass spectrometry

Antioxidant properties and detailed polyphenol profiling of European hornbeam (Carpinus betulus L.) leaves by multiple antioxidant capacity assays and high-performance liquid chromatography/multistage electrospray mass spectrometry

Preliminary Phytochemical analysis and In vitro Antioxidant, FTIR Spectroscopy, Anti-diabetic activity of Acacia catechu ethanolic seed extract

Floral Extracts of Allamanda Blanchetii and Allamanda Cathartica Are Comparatively Higher Resource of Anti-Oxidants and Polysaccharides Than Leaf and Stem Extracts

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