Estimation of Total Phenolic and Total Flavonoid Content and Assessment of in vitro Antioxidant Activity of Extracts of Hamelia patens Jacq. Stems

Surana, Ajaykumar Rikhabchand; Kumbhare, Manoj Ramesh; Wagh, Rajendra D.

doi:10.3923/rjphyto.2016.67.74

Cited by 22 publications

(16 citation statements)

References 27 publications

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“…TPC of the tomato extract was estimated following the method of Surana et al (2016) , with minor modifications. One millilitre (1 mL) of the stock extract was mixed with 1 mL of Gallic Acid (GA) standard solution in a 25-mL volumetric flask.…”

Section: Methodsmentioning

confidence: 99%

Non-destructive assessment of flesh firmness and dietary antioxidants of greenhouse-grown tomato (Solanum lycopersicum L.) at different fruit maturity stages

Alenazi

Shafiq

Alsadon

et al. 2020

Saudi Journal of Biological Sciences

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Non-destructive methods have been widely recognized for evaluating fruit quality traits of many horticultural crops and food processing industry. Destructive (analytical) test, and non-destructive evaluation of the quality traits were investigated and compared for ‘Red Rose’ tomato ( Solanum lycopersicum L.) fruit grown under protected environment. Fresh tomato fruit at five distinctive maturity stages namely; breaker (BK), turning (TG), pink (PK), light-red (LR), and red (RD) were labeled and scanned using the handheld near infra-red (NIR) enhanced spectrometer at a wavelength range of 285–1200 nm. The labeled tomato samples were then measured analytically for flesh firmness, lycopene, β-carotene, total phenolic content (TPC) and total flavonoids content (TFC). The results revealed that quality traits could be estimated using NIR spectroscopy with a relatively high coefficient of determination (R 2 ): 0.834 for total phenolic content, 0.864 for lycopene, 0.790 for total flavonoid content, 0.708 for β-carotene; and 0.679 for flesh firmness. The accumulation of Lyco and β-Car rapidly increased in tomatoes harvested between the TG and the LR maturity stages. Harvesting tomatoes at BK maturity stage resulted in significantly higher flesh firmness than harvesting at the later maturity stages. Tomato fruits had the lowest TPC and TFC contents at the earliest maturity stage (BK), while they had intermediate TPC and TFC levels at LR and RD maturity stages. NIR spectroscopic measurements of fruit firmness and lipophilic antioxidants in tomato fruit at various maturity stages were partially in accordance with those estimated by destructive (analytical) methods. Based on these findings, we recommend using non-destructive NIR spectroscopy as an effective tool for predicting tomato fruit quality during harvest stage and postharvest processing.

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Section: Methodsmentioning

confidence: 99%

Non-destructive assessment of flesh firmness and dietary antioxidants of greenhouse-grown tomato (Solanum lycopersicum L.) at different fruit maturity stages

Alenazi

Shafiq

Alsadon

et al. 2020

Saudi Journal of Biological Sciences

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“…One more investigation state that the antioxidative activity of pet ether, chloroform and methanol extracts of Hamelia patens stem were evaluated along with total phenolic and flavonoid content. All these extracts of Hamelia showed significant reactive oxygen scavenging activity against DPPH radical, nitric oxide, hydrogen peroxide and also act as metal chelating [38].…”

Section: Anti-oxidant Activitymentioning

confidence: 99%

A Phytochemical and Ethnopharmacological Recapitulation on Hamelia patens

et al. 2020

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The world health organization reports that 80% of the population living within the developing countries depends basically on traditional medicine for his or her primary health care. Quite half the entire world's population still depends entirely on plants for medicines, and plants provides the active ingredients to the most traditional medical products. Hamelia patens that belong to the family Rubiaceae, is mainly found in tropical and sub-tropical areas. It is used in folk medicine against a wide range of diseases such as athlete’s foot, skin problems, insect sting, psychiatric disorder, rheumatism, headache, asthma, dysentery, menses, ovarian and uterine disorders. Hamelia consists of an important bioactive constituent such as oxindole alkaloids, flavonoids, and phenolic content. Due to the presence of chlorogenic acid and epicatechin constituent in the methanolic extract of Hamelia patens, there is a noticeable anti-hyperglycemic activity as well as it possesses antioxidant activity. Acute and sub-acute toxicity study has been performed on Hamelia patens which shows that it is safe and can be used for humans. Against fungi and bacteria, the ethanol leaf extract of Hamelia has a maximum inhibitory effect. The plant has various therapeutic effects like anti-inflammatory, analgesic, anti-diarrheal, anthelmintic, antidepressant, hepatoprotective, antiurolithiatic, diuretic, wound healing, and many others. In this article, we have discussed chemical constituent, pharmacological activity and traditional use of Hamelia patens.

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“…The 1 ml of aliquots ethanol, methanol and acetone extracts and standard Gallic acid (10,20,40,60,80, 100 μg/ml) were positioned into the test tubes and 5ml of distilled water and 0.5 ml of Folin Ciocalteu's reagent was mixed and shaken. After 5 minutes, 1.5 ml of 20 % sodium carbonate was added and volume made up to10 ml with distilled water.…”

Section: Total Phenol Content Was Estimated By Folin Ciocalteu's Methodsmentioning

confidence: 99%

“…The scavenging activity of methanol, ethanol, acetone and water extracts of seaweeds was determined according to standard protocol (Surana et al 2016). Briefly, 2.0 ml of 0.16 mM DPPH solution in methanol was added to the test tube containing 2.0 ml aliquots of sample.…”

Section: Dpph Radical Scavenging Activity Assaymentioning

confidence: 99%

Screening and Partial Characterization of Natural Antioxidants from Seaweeds Collected From, Rameshwaram Southeast Coast of India

2020

Journal of Marine Science Research and Oceanography

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The aim of this work is to estimate in-vitro antioxidant activities such as total antioxidant capacity, free radicals (DPPH) scavenging activity, hydrogen peroxides (H2 O2 ) scavenging activity, total reducing power scavenging activity and total phenols, of various extracts of seaweeds such as aqueous, acetone, ethanol, methanol, and pigments content by spectrophotometric method, of 33 seaweeds species among which 11 Chlorophyta, 11 Phaeophyceae and 11 Rhodophyta, collected during 2016 from two stations viz. Olaikuda and Vadakkadu, at Rameshwaram, southeast coast of India. Among four different extracts aqueous extracts from all seaweeds had minimum activity than acetone, methanol and ethanol. The Rhodophyta and Phaeophyceae had high antioxidant activity in comparing to Chlorophyta. The highest total antioxidant activity was found in acetone extract from Turbinaria decurrens (98.97±0.00%), followed by its methanol extract (98.81±0.60%) and ethanol extract (98.58±0.53%). The highest reducing power and H2 O2 scavenging activity were found in acetone extract of Caulerpa racemosa (383.25±1.04%), and methanol extract from Caulerpa racemosa var. macrophysa (24.91±0.49%). The methanol extract from Caulerpa scalpelliformis contained the highest total phenol (85.23±0.12%). The Chloro-a and Chloro-b contents were the highest in Gracilaria foliifera (13.69±0.38% mg/gm dry wt.) and Caulerpa racemosa var. macrophysa (9.12 ±0.12% mg/gm dry wt.) likewise carotenoid was also the highest in Gracilaria foliifera (0.054±0.0003% mg/gm dry wt.) and Caulerpa racemosa var. macrophysa (0.04 ±0.002% mg/gm dry wt.). The partial characterization of the extract which contents the highest activity was done by UV-Visible Spectrophotometer, FTIR, and NMR. The functional groups and all the possible compounds present in the extract were partially characterized. It can be concluded from this study, that some seaweed extract can be used for natural antioxidant production, after further characterization to negotiate the side effect of synthetic, market available antioxidants.

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Estimation of Total Phenolic and Total Flavonoid Content and Assessment of in vitro Antioxidant Activity of Extracts of Hamelia patens Jacq. Stems

Cited by 22 publications

References 27 publications

Non-destructive assessment of flesh firmness and dietary antioxidants of greenhouse-grown tomato (Solanum lycopersicum L.) at different fruit maturity stages

Non-destructive assessment of flesh firmness and dietary antioxidants of greenhouse-grown tomato (Solanum lycopersicum L.) at different fruit maturity stages

A Phytochemical and Ethnopharmacological Recapitulation on Hamelia patens

Screening and Partial Characterization of Natural Antioxidants from Seaweeds Collected From, Rameshwaram Southeast Coast of India

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