Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants

Kumar, Deepak; Jamwal, Anupam; Madaan, Reecha; Kumar, Suresh

doi:10.15415/jptrm.2014.21006

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…The concentrated methanol extract was further used for the phytochemical analysis. Phytochemicals such as flavonoids, phenols and steroids were quantified using the procedure reported by (Kumar et al, 2014;Clemensen et al, 2022).…”

Section: Quantification Of Secondary Metabolitesmentioning

confidence: 99%

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

Natarajan,

Rangasamy,

Santhanu

et al. 2023

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Withania somnifera is a medicinal ayurvedic plant native to India. Lead (Pb) is a toxic heavy metal which has no biological function in plants. The aim of the present study is to investigate the influence of heavy metal Pb on W. somnifera at cellular level under controlled conditions. The role of Pb on shoots of W. somnifera was determined in terms of biomass, secondary metabolite production (High Performance Thin Layer Chromatography) and phytochemical quantification. Influence and accumulation of Pb at the cellular level were recognized using UV visible spectroscopy, field emission scanning electron microscope and x-ray diffraction analysis. The current result showed that the concentration up to 2.4 mM Pb for 7 days of exposure showed higher biomass and withaferin A yield compared to control shoots. On the other hand, other secondary metabolites such as flavonoids, phytosterols and phenols levels were reduced compared to control shoots. In the morphological study, Pb concentration in the analysed sample was determined as 2%. The probability of the nanoparticle nature of the bioaccumulated lead also verified using spectroscopy and x-ray diffraction analysis. To date, we are the first to report on the influence of Pb on in vitro shoot cultures of W. somnifera.

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Section: Quantification Of Secondary Metabolitesmentioning

confidence: 99%

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

Natarajan,

Rangasamy,

Santhanu

et al. 2023

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“…Estimation of Total Phenols: The total phenolic content was determined according to the following method 25,26,27 . Ten microlitre aliquots of the extracts (2mg/2ml) were taken in test tubes and made up to the volume of 1 ml with distilled water.…”

Section: Estimation Of Total Flavonoidsmentioning

confidence: 99%

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2021

IJPSR

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The plant Scoparia dulcis Linn. belongs to the family Scrophulariaceae, is a native of tropical America, commonly called a sweet broom. The traditional healers identified its uses as anti-diabetic, antipyretic, antihypertensive, diuretic, and they have developed many promising traditional medicines and formulations from this plant. The main objective of this present study reveals about the phytoconstituents present in the leaves extract by preliminary phytochemical study, isolation of active compounds by column chromatography, and characterize the isolated compound by spectral studies like UV, IR, NMR, and MASS spectra. The preliminary phytochemical study reveals that the plant possesses more phytochemicals such as carbohydrates, alkaloids, tannins, phenols, flavonoids, vitamin C, and amino acids. The quantitative estimation shows that it consist of a considerable quantity of flavonoids, Phenols, Vitamin C. The methanol extract was prepared and applied in column chromatography to isolate the phytoconstituents. A dull white crystalline compound was isolated in the fraction of Acetone: Ethanol (60:40), (40:60) ratio. This compound was subjected to spectral studies. The spectral studies indicate the presence of -OH, C=O, and C-H groups from the NMR signal. It shows the presence of methyl proton and α -CH 2 proton. Based on the spectral studies, the chemical nature of the isolated compound may be a heptadeconic acid with the molecular formula CH 3 -(CH 2 ) 15 -COOH. Future studies also can go for further identification of new compounds and their medicinal values. INTRODUCTION: Phytochemicals are the complex mixture of chemicals naturally synthesized by plants, and they are a very important base for phytotherapy and nutritional sciences. Some are completely characterized, and some are incompletely characterized and have recently been subject to scientific scrutiny 1 . Abundant of photochemical are present in plant sources without knowing their chemical nature and therapeutic values.

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“…Total flavonoid was determined by aluminium chloride method 21 as follows: The 100µL extract solution was taken and added 300 µL Hexane. Then 100 µLAlCl3 (10%), 100 µL Na-K tartrate and 300 µL distilled water were added sequentially.…”

Section: Total Flavonoid Contentmentioning

confidence: 99%

In Vitro Antidiabetic Activity and Phytochemical Analysis of Lumnitzera Racemosa Leaves

Ranjana¹,

Jadhav²,

Dhavan³

et al. 2019

Int. Res. J. Pharm.

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The present study was designed to evaluate the antidiabetic property and phytochemical composition of Lumnitzera racemosa leaves. Mangrove plant L. racemosa was collected in the month of May from Bhatye beach area Ratnagiri coast, Maharashtra, India. Leaves separated, powdered and exhaustive extraction was carried out in methanol, ethanol, ethyl acetate, and pet ether solvents with increasing polarity using Soxhlet apparatus and in vitro antidiabetic activity was determined by α-amylase and α-glucosidase inhibitory assays using acarbose as standard. Phytochemical analysis was carried out as per Wagner and Bladt (1996). Results have confirmed antidiabetic activity in methanol extract which was comparable to standard acarbose followed by ethyl acetate, ethanol and pet ether extracts. The phytochemical results showed the presence of flavonoid, saponin, essential oil, alkaloid, anthraquinones, bitter principles, phenol, triterpenoids, coumarin, and tannin in the leaves.

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Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants

Cited by 10 publications

References 13 publications

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

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In Vitro Antidiabetic Activity and Phytochemical Analysis of Lumnitzera Racemosa Leaves

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