Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. in MCF-7 cells

Lee, Young Min; Kim, Jung Bong; Bae, Ji Hyun; Lee, Jong Suk; Kim, Pan-Soo; Jang, Hwan Hee; Kim, Haeng Ran

doi:10.1186/1472-6882-12-260

Cited by 27 publications

(26 citation statements)

References 36 publications

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“…Phytoestrogens are chemical compounds synthesized by plants and are obtained naturally in a wide range of foods, and can have estrogenic effects (25)(26)(27)(28). The authors previously reported that low-density blackcurrant anthocyanins and BCE exhibit phytoestrogenic activity mediated via estrogen receptor α signaling (13).…”

Section: Discussionmentioning

confidence: 99%

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Nanashima

Horie

Chiba

et al. 2017

Molecular Medicine Reports

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Blackcurrants (Ribes nigrum L., Grossulariaceae) possess a high content of anthocyanin polyphenols, which have been demonstrated to exhibit beneficial effects on health due to their antioxidant and anticarcinogenic prope-rties. The present study investigated novel functions of anthocyanin‑rich blackcurrant extracts (BCEs) in a healthy mammary epithelial cell line, MCF10A. The percentages of viable cells were 85, 75, 53 and 31% following exposure to 50, 100, 200 and 400 µg/ml BCE, respectively. The half‑maximal response concentration of BCE was 237.7 µg/ml. Microarray and Ingenuity® Pathway Analysis demonstrated that BCE downregulated cell cycle signaling, including upstream genes with mitotic roles such as polo‑like kinase signaling. BCE increased the number of cells in the G0/G1 phase and decreased the number of cells in the S and G2/M phases. Alkaline comet assays demonstrated that 50 and 100 µg/ml BCE induced DNA damage in a dose‑dependent manner. Cultures treated with 0, 50, and 100 µg/ml BCE contained 4.6, 13.4 and 16.0% apoptotic cells, respectively. As compared with the untreated cultures (1.9%), the number of necrotic cells increased in the 100 µg/ml BCE‑treated cultures (from 1.9 to 4.3%) but not in the 50 µg/ml BCE‑treated cultures. Reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that BCE reduced mRNA expression of the genomic caretaker lysine‑specific demethylas 5B (KDM5B). The results suggested that blackcurrant anthocyanins may act as cell arrest and death inducers via KDM5B downregulation in healthy breast cells.

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Section: Discussionmentioning

confidence: 99%

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Nanashima

Horie

Chiba

et al. 2017

Molecular Medicine Reports

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“…A number of estrogen target genes have been shown to be involved in regulating the proliferation of ER-positive breast cancer cells and modulating estrogen sensitivity in a gene-specific manner (29–31). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of γ- and δ-Tocopherols on Estrogen-Stimulated Breast Cancer In Vitro and In Vivo

Bak

Gupta

Wahler

et al. 2017

Cancer Prevention Research

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Estrogens have been implicated as complete carcinogens for breast and other tissues through mechanisms involving increased cell proliferation, oxidative stress and DNA damage. Because of their potent antioxidant activity and other effects, tocopherols have been shown to exert anti-tumor activities in various cancers. However, limited information is available on the effect of different forms of tocopherols in estrogen-mediated breast cancer. To address this, we examined the effects of α-, γ- and δ-tocopherols as well as a natural γ-tocopherol rich mixture of tocopherols, γ-TmT, on estrogen-stimulated MCF-7 cells in vitro and in vivo. For the in vivo studies, MCF-7 cells were injected into the mammary fat pad of immunodeficient mice previously implanted with estrogen pellets. Mice were then administered diets containing 0.2% α-, γ-, δ-tocopherol or γ-TmT for 5 weeks. Treatment with α-, γ-, δ-tocopherols and γ-TmT reduced tumor volumes by 29% (p<0.05), 45% (p<0.05), 41% (p<0.05) and 58% (p<0.01), as well as tumor weights by 20%, 37% (p<0.05), 39% (p<0.05) and 52% (p<0.05), respectively. γ- and δ-Tocopherols and γ-TmT inhibited the expression of cell proliferation-related genes such as cyclin D1 and c-Myc, and estrogen-related genes such as TFF/pS2, cathepsin D and progesterone receptor in estrogen-stimulated MCF-7 cells in vitro. Further, γ- and δ-tocopherols decreased the levels of estrogen-induced oxidative stress and nitrosative stress markers, 8-hydroxy-2’-deoxyguanosine and nitrotyrosine, as well as the DNA damage marker, γ-H2AX. Our results suggest that γ- and δ-tocopherols and the γ-tocopherol rich mixture are effective natural agents for the prevention and treatment of estrogen-mediated breast cancer.

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“…According to Carson and Rittmaster, TGF-β activity, which modulates apoptosis, is also influenced by dihydrotestosterone (DHT) [143,144]. More recently, the importance of DHT has been known to act as androgens and to be metabolized to 5α-androstane-3β-17β-diol (3βAdiol), androgens, which are a ligand for estrogen receptor [145][146][147][148][149][150].…”

Section: Discussionmentioning

confidence: 99%

Transforming Growth Factor Beta of Testis Germinal Cell in Guinea Pig (Cavia Porcellus) After Exposure to Methanol Extract of the Seeds of Bitter Melon (Momordica Charantia) and Depot Medroxyprogesterone Acetate

Ilyas

Hutahaean

Nursal

2018

Asian J Pharm Clin Res

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Objective: The discovery of male contraceptive drugs continues to be pursued, due to the very small participation of men associated with the lack of contraceptiveÐptions for men. The combination of methanol extract of bitter melon seed and depot medroxyprogesterone acetate (DMPA) becomes the choice that currently being pursued to be applied to men. Methods:The use of guinea pigs as experimental animals conducted research using experimental methods with complete randomized design. The study was divided into four control groups i.e K0; dimethylsulfoxide (DMSO) for 0 week (4 h),K1; Control group of DMSO for 4 weeks, K2; Control group of DMSO for 8 weeks, K3; Control group of DMSO for 12 weeks, and four treatment groups, i.e: group P0; bitter melon seed extract of 50 mg/100g body weight/day for 0 week (4 h), group P1; Bitter melon seed extract of 50 mg/100g BW/day for 4 weeks+DMPA, group P2; Bitter melon seed extract of 50 mg/100g BW/day for 8 weeks+DMPA, P3 group; Bitter melon seed extract of 50 mg/100g BW/day for 12 weeks+DMPA.Results: There was a significant effect (p<0.05) methanol extract of bitter melon seed to increase the transforming growth factor expression -β expression. Conclusion:The methanol extract of bitter melon seed was able to be candidate for herbal contraception.

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Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. in MCF-7 cells

Cited by 27 publications

References 36 publications

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Inhibitory Effects of γ- and δ-Tocopherols on Estrogen-Stimulated Breast Cancer In Vitro and In Vivo

Transforming Growth Factor Beta of Testis Germinal Cell in Guinea Pig (Cavia Porcellus) After Exposure to Methanol Extract of the Seeds of Bitter Melon (Momordica Charantia) and Depot Medroxyprogesterone Acetate

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