Estrogenic and anti-estrogenic effects of wood extractives present in pulp and paper mill effluents on rainbow trout

Orrego, Rodrigo; Guchardi, John; Krause, Rachelle; Holdway, Douglas A.

doi:10.1016/j.aquatox.2010.04.016

Cited by 46 publications

(42 citation statements)

References 33 publications

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“…Thanks to the developments occurred in the P&P treatment methods; toxicity of the final discharged effluents has been greatly decreased. However, such pollutants continue to be found in the final treated effluents (Orrego et al, 2010). This is mainly due to the remaining technical problems which lead to incomplete degradation as well as economic limitations of some effective P&P wastewater treatment methods.…”

Section: Advances In Pandp Wastewater Treatmentsmentioning

confidence: 99%

Review on recent developments on pulp and paper mill wastewater treatment

Kamali

Khodaparast

2015

Ecotoxicology and Environmental Safety

445

246

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Section: Advances In Pandp Wastewater Treatmentsmentioning

confidence: 99%

Review on recent developments on pulp and paper mill wastewater treatment

Kamali

Khodaparast

2015

Ecotoxicology and Environmental Safety

445

246

View full text Add to dashboard Cite

“…and that effluents contain ligands for sex steroid receptors (Hewitt et al, 2006; MacLatchy and Van Der Kraak, 1995). Although steroid-dependent pathways clearly are affected (Orrego et al, 2010), disruption of other pathways could impair fish reproduction (Basu et al, 2009; Popesku et al, 2010; Van der Kraak et al, 1992), because many effluents are strongly anti-reproductive but are neither estrogenic nor androgenic in standard assays . In vitro studies have revealed that extracts from effluents and trees contain chemicals that can interfere with the GABA synthesis enzyme glutamic acid decarboxylase and the DA metabolism enzyme monoamine oxidase (Basu et al, 2009; Milestone et al, 2012; Waye et al, 2013).…”

Section: Phytoestrogens and Other Phytochemicalsmentioning

confidence: 99%

Current concepts in neuroendocrine disruption

León-Olea

Martyniuk

Orlando

et al. 2014

General and Comparative Endocrinology

127

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In the last few years, it has become clear that a wide variety of environmental contaminants have specific effects on neuroendocrine systems in fish, amphibians, birds and mammals. While it is beyond the scope of this review to provide a comprehensive examination of all of these neuroendocrine disruptors, we will focus on select representative examples. Organochlorine pesticides bioaccumulate in neuroendocrine areas of the brain that directly regulate GnRH neurons, thereby altering the expression of genes downstream of GnRH signaling. Organochlorine pesticides can also agonize or antagonize hormone receptors, adversely affecting crosstalk between neurotransmitter systems. The impacts of polychlorinated biphenyls are varied and in many cases subtle. This is particularly true for neuroedocrine and behavioral effects of exposure. These effects impact sexual differentiation of the hypothalamic-pituitary-gonadal axis, and other neuroendocrine systems regulating the thyroid, metabolic, and stress axes and their physiological responses. Weakly estrogenic and anti-androgenic pollutants such as bisphenol A, phthalates, phytochemicals, and the fungicide vinclozolin can lead to severe and widespread neuroendocrine disruptions in discrete brain regions, including the hippocampus, amygdala, and hypothalamus, resulting in behavioral changes in a wide range of species. Behavioral features that have been shown to be affected by one or more these chemicals include cognitive deficits, heightened anxiety or anxiety-like, sociosexual, locomotor, and appetitive behaviors. Neuroactive pharmaceuticals are now widely detected in aquatic environments and water supplies through the release of wastewater treatment plant effluents. The antidepressant fluoxetine is one such pharmaceutical neuroendocrine disruptor. Fluoxetine is a selective serotonin reuptake inhibitor that can affect multiple neuroendocrine pathways and behavioral circuits, including disruptive effects on reproduction and feeding in fish. There is growing evidence for the association between environmental contaminant exposures and diseases with strong neuroendocrine components, for example decreased fecundity, neurodegeneration, and cardiac disease. It is critical to consider the timing of exposures of neuroendocrine disruptors because embryonic stages of central nervous system development are exquisitely sensitive to adverse effects. There is also evidence for epigenetic and transgenerational neuroendocrine disrupting effects of some pollutants. We must now consider the impacts of neuroendocrine disruptors on reproduction, development, growth and behaviors, and the population consequences for evolutionary change in an increasingly contaminated world. This review examines the evidence to date that various so-called neuroendocrine disruptors can induce such effects often at environmentally-relevant concentrations.

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“…Isoprenoid biosynthesis is the necessary precursor for cholesterol synthesis, which serve as templates for sex steroid synthesis. Genes upstream of the synthesis of cholesterol have previously been found to be upregulated by androgen exposure [35], lending support to the hypothesis that chemicals present in paper mill effluents are acting to disrupt the normal metabolism of steroids and hormones in a manner similar to androgen exposures [31].…”

Section: Discussionmentioning

confidence: 54%

“…However, our findings match results from other fish species exposed to paper mill effluent extracts. The Vtg protein was significantly increased in rainbow trout ( Oncorhynchus mykiss ) after intraperitoneal (IP) injection with primary extracts [31] and after exposure to 100% combined mill outfall (CMO) and in 10% untreated kraft effluent exposures to fathead minnow [32]. While gene and protein levels are not always directly correlated, the upregulation of this protein in rainbow trout and fathead minnow and the gene in mosquitofish may be due to the presence of phytoestrogens in these effluents such as β-sitosterol [6].…”

Section: Discussionmentioning

confidence: 99%

Exposure to Paper Mill Effluent at a Site in North Central Florida Elicits Molecular-Level Changes in Gene Expression Indicative of Progesterone and Androgen Exposure

et al. 2014

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Endocrine disrupting compounds (EDCs) are chemicals that negatively impact endocrine system function, with effluent from paper mills one example of this class of chemicals. In Florida, female Eastern mosquitofish (Gambusia holbrooki) have been observed with male secondary sexual characteristics at three paper mill-impacted sites, indicative of EDC exposure, and are still found at one site on the Fenholloway River. The potential impacts that paper mill effluent exposure has on the G. holbrooki endocrine system and the stream ecosystem are unknown. The objective of this study was to use gene expression analysis to determine if exposure to an androgen receptor agonist was occurring and to couple this analysis with in vitro assays to evaluate the presence of androgen and progesterone receptor active chemicals in the Fenholloway River. Focused gene expression analyses of masculinized G. holbrooki from downstream of the Fenholloway River paper mill were indicative of androgen exposure, while genes related to reproduction indicated potential progesterone exposure. Hepatic microarray analysis revealed an increase in the expression of metabolic genes in Fenholloway River fish, with similarities in genes and biological processes compared to G. holbrooki exposed to androgens. Water samples collected downstream of the paper mill and at a reference site indicated that progesterone and androgen receptor active chemicals were present at both sites, which corroborates previous chemical analyses. Results indicate that G. holbrooki downstream of the Fenholloway River paper mill are impacted by a mixture of both androgens and progesterones. This research provides data on the mechanisms of how paper mill effluents in Florida are acting as endocrine disruptors.

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Estrogenic and anti-estrogenic effects of wood extractives present in pulp and paper mill effluents on rainbow trout

Cited by 46 publications

References 33 publications

Review on recent developments on pulp and paper mill wastewater treatment

Review on recent developments on pulp and paper mill wastewater treatment

Current concepts in neuroendocrine disruption

Exposure to Paper Mill Effluent at a Site in North Central Florida Elicits Molecular-Level Changes in Gene Expression Indicative of Progesterone and Androgen Exposure

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