Estrogenic responses of larval sunshine bass (Morone saxatilis × M. Chrysops) exposed to New York city sewage effluent

Answering questions on the impact of recycled water on wildlife usingGambusia holbrookias a surrogate

“…These data are also consistent with previous investigations of the effects of endocrine disruption on fish from waters receiving sewage effluent (Batty and Lim 1999;Diniz et al 2005a, b;Gagné et al 2004;Todorov et al 2002) and stormwater (Baun et al 2006;Eriksson et al 2007). Our data therefore confirm that even with tertiary treated effluent and stormwater from a township without secondary industries, fish may suffer endocrine disruption within receiving waters.…”

“…The responses of aquatic-dependent wildlife (e.g., amphibians, Carey 2000; fish, Jobling and Tyler 2003; marine invertebrates, Depledge and Billinghurst 1999), feral species (e.g., Batty and Lim 1999;Diniz et al 2005a, b;Todorov et al 2002), and the mussel Elliptio complanata, (Gagné et al 2004) to treated sewage effluent have been widely reported. Another source of endocrine disrupting contaminants, stormwater, contains chlorinated pesticides, poly-aromatic hydrocarbons (PAHs), and polychlorinated compounds.…”

Variable susceptibility and response to estrogenic chemicals in Menidia menidia

“…Previous work has demonstrated that effluent from several New York City sewage treatment plants with concentrations of E 1 and E 2 of from 5 to 13 ng l -1 and estrogenic nonylphenol ethoxylates is estrogenic to larval hybrid striped bass (McArdle et al 2000, Todorov et al 2002. Furthermore, surveys of juvenile winter flounder Pseudopleuronectes americanus have demonstrated highly female-biased sex ratios in Jamaica Bay, western Long Island, as opposed to a sex ratio of 0.5 in Shinnecock Bay off eastern Long Island (McElroy et al 2006).…”

Biomarker responses and reproductive toxicity of the effluent from a Chinese large sewage treatment plant in Japanese medaka (Oryzias latipes)

“…At present study, purified medaka VTG (our laboratory) was loaded into the gel, the coefficient between the integrated optical density (IOD) of the purified medaka VTG band and the amount of VTG applied to the electrophoretic lanes is 0.9925. Relative induced VTG levels of plasma were quantified by the IOD image analysis (Allner et al, 1999;Todorov et al, 2002) using an imaging and analysis software Quantity One version 4.3.0 (Bio-Rad). Briefly, prior to electrophoresis, blood plasma samples from all treatments were diluted as suitable factors to ensure clear bands and then were subjected to SDS-PAGE, the integrated optical density (IOD) of the VTG band was measured by above computer program.…”