Estrone Sulfate and Progesterone Profiles During Late Gestation in Recipient Cows Transferred Embryos Produced by Nuclear Transfer and In Vitro Fertilization

Abstract

Abstract. The aim of present experiment was to evaluate the plasma concentrations of estrone sulfate (E1S) and progesterone (P4) during late gestation in recipient cows transferred embryos produced by nuclear transfer (NT) and in vitro fertilization (IVF). Blood samples were collected from recipients transferred embryos produced by NT (n=9) and IVF (n=13) at 160, 220, 240, 260 and 270 d of gestation and then at 5 d intervals until parturition. Plasma samples were analyzed for E1S and P4 by ELISA. One NT and three IVF cows aborted between days 220 and 260 of gestation. Two NT and one IVF cow had prolonged gestation (over 290 d). One IVF cow had an overweight fetus (50 kg) after abortion (257 d). The patterns of changes in the concentrations of E1S during late gestation in the NT and IVF cows were almost identical. The NT and IVF cows that aborted had prolonged gestation and much higher E1S concentrations than the average. One NT cow aborted after 220 d of gestation and had a sudden high increase in its E1S concentration from 160 d to 220 d of gestation. The NT and IVF cows that had prolonged gestation also had significantly higher (P<0.05) P4 concentrations than the average. These results raise the possibility that the E1S and P4 profiles can be used to monitor some late gestational problems, such as higher birth weight, abortion and prolonged gestation.

Reticulo-rumen temperature as a predictor of calving time in primiparous and parous Holstein females

“…tion is greater due to its production by the corpus luteum, with blood plasma values of 2.5 to 14.0 ng/ mL from the beginning to the final third of gestation in which there is a gradual drop in the concentration to 1.0 and 1.5 ng/mL of blood plasma 1 d before calving (Wettemann and Hafs, 1973;Lammoglia et al, 1997;Shah et al, 2007). Nevertheless, estrogen concentration is between 3 and 30 pg/mL in blood plasma until midway through gestation, with a subsequent increase to between 250 and 295 pg/mL 282 d into gestation (Smith et al, 1973;Patel et al, 1999).…”

Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

“…On the 7th day after observation of estrus, termed blastocysts were transferred non-surgically into the uterine lumen, ipsilateral to the corpus luteum, of surrogates that had been confirmed to be in the luteal phase (Shah et al, 2007).…”

Plasma estrone sulfate, clinical biochemistry, and milk yield of dairy cows carrying a fetus from a bull or its clone

“…According to Shah et al [20], plasma E 1 SO 4 reflects the degree of fetoplacental development. Guibault et al [21] reported a correlation (r ¼ 0.64, P < 0.01) between CBW and maternal concentrations of antepartum estrone sulfate evidencing how the plasma E 1 SO 4 in the gestating cow is determined by the genetics of the conceptus (probably as a result of the fetus weight).…”