Estudo de validação comparativo entre as técnicas Elisa e RIFI para diagnosticar Leishmania sp em cães errantes apreendidos no município de Campos dos Goytacazes, Estado do Rio de Janeiro

Távora, Mariane Pinto Fernandes; Pereira, Maria Angélica Vieira da Costa; Silva, Valmir Laurentino; Vita, Gilmar Ferreira

doi:10.1590/s0037-86822007000400023

Cited by 7 publications

(6 citation statements)

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“…Reactive samples according to serological diagnosis methods confirm a previous exposure to Leishmania antigens. Considering that the study was performed in an endemic region for CL and that all animals were apparently healthy, the results that were obtained corroborate the literature ( Távora et al, 2007 ).…”

Section: Resultssupporting

confidence: 87%

High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil

Cavallieri,

Katto,

Holsback

et al. 2023

Rev. Bras. Parasitol. Vet.

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Leishmaniasis is an anthropozoonosis with vector transmission, and knowledge regarding the occurrence of this parasitosis in sentinels can contribute to infection and disease control measures in humans. The objectives of this study were to evaluate the occurrence of Leishmania exposure and infection in dogs from urban and rural areas in the North Pioneer Mesoregion of the state of Paraná, to evaluate possible risk factors, and to analyze the statistical agreement between the serological techniques that were used. Using a convenience sampling, serum and whole blood samples were collected to perform serological and molecular assays, respectively. The enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT) identified 29/204 (14.2%) and 20/204 (9.8%) seropositive dogs, respectively. Five dogs (2.4%) were seropositive for both serological tests, and four dogs presented high titers in the IFAT. None of the samples tested positive for Leishmania spp. DNA according to polymerase chain reaction analysis. No factors were significantly associated with infection. Leishmania parasites circulate in urban and rural dogs in the North Pioneer Mesoregion of the state of Paraná. Despite the absence of clinical cases, seropositive animals with high antibody titers should serve as a warning to the local population that should be properly informed regarding the prevention.

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Section: Resultssupporting

confidence: 87%

High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil

Cavallieri,

Katto,

Holsback

et al. 2023

Rev. Bras. Parasitol. Vet.

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“…Both of these tests were available through the freeware BioEstat version 5.0, and were used with 5% statistical significance. Co-positivity was calculated as a/(a + c), co-negativity was expressed as d/(b + b) and raw concordance was described as (a + d)/(a + b + c + d) (TÁVORA et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Molecular and parasitological detection of Leishmania spp. in dogs caught in Palmas, TO, Brazil

Teles

Agostini

Bigeli

et al. 2012

Rev. Bras. Parasitol. Vet.

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This study evaluated occurrences of Leishmania infantum in dogs in the municipality of Palmas, Tocantins, comparing diagnostic data obtained using the polymerase chain reaction (PCR) and parasitological diagnosis. Blood samples and lymph node aspirates were collected from 63 dogs of males and females and various ages and races, with or without owners, between August 2009 and June 2010. Slides containing smears of lymph node aspirates were stained with Giemsa stained. In PCR, the 145 bp target sequence of the LT1 fragment, located in the Leishmania donovani kDNA minicircle was detected using the RV1 and RV2 oligonucleotide primers. The chi-square test revealed that there was a significant relationship between the symptoms and dogs that were positive for visceral leishmaniasis (VL). The parasitological investigation showed concordance of 66.7% with PCR on blood and 84.1% with PCR on lymph node aspirate. In addition to these tests, evaluations of the diagnoses in parallel and in series were conducted, which showed concordances with the parasitological test of 76.2% and 74.6%, respectively. The results make it possible to suggest that PCR on lymph nodes should be used in evaluating large populations (surveys) and that the parasitological test should be used for initial clinical evaluations in veterinary consultation offices.Keywords: PCR, blood, lymph node aspirate, parasite, diagnosis. ResumoAvaliou-se a ocorrência de Leishmania infantum em cães do município de Palmas-TO, comparando dados diagnósticos obtidos pela Reação em Cadeia da Polimerase (PCR) e pelo diagnóstico parasitológico. Foram coletadas amostras de sangue e de aspirado de linfonodo de 63 cães machos e fêmeas, várias idades e raças, domiciliares ou não de agosto de 2009 a junho de 2010. As lâminas contendo esfregaço dos aspirados de linfonodos foram coradas pelo corante Giemsa. Na PCR, a sequência alvo de 145 pb do fragmento LT1, situado no minicírculo do kDNA do grupo Leishmania donovani, foi detectada através dos oligonucleotídeos iniciadores RV1 e RV2. O teste χ 2 (Qui-quadrado), demonstrou haver relação significativa entre a sintomatologia e a positividade dos cães para Leishmaniose Visceral (LV). O exame parasitológico mostrou uma concordância de 66,7% com a PCR em sangue e 84,1% com a PCR de aspirado de linfonodo. Além destas análises, houve a avaliação dos diagnósticos em paralelo e em série, onde as concordâncias com o exame parasitológico foram de 76,2% e 74,6%, respectivamente. Os resultados permitem sugerir a utilização da PCR de linfonodos na avaliação de grandes populações (inquéritos), e o exame parasitológico para a avaliação clínica inicial em consultórios veterinários.Palavras-chaves: PCR, sangue, aspirado de linfonodos, parasita, diagnóstico.

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“…O teste ELISA é usado principalmente para detecção de anticorpos em inquéritos soroepidemiológicos por ser prático, menos oneroso e rápido. A RIFI apresenta maior grau de especificidade e o teste ELISA um maior grau de sensibilidade (TÁVORA et al, 2007). A utilização de testes altamente sensíveis, que detectam o maior número possível de animais infectados, seria o mais recomendado para ações de saúde pública, pois reduziriam o potencial de transmissão.…”

Section: Comparação Entre As Técnicas Rifi E Elisaunclassified

Leishmania infection: painful or painless?

et al. 2016

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The complex life cycle and immunopathological features underpinning the interaction of Leishmania parasites and their mammalian hosts poses frequent poorly explored and inconclusively resolved questions. The altered nociceptive signals over the course of leishmaniasis remain an intriguing issue for nociceptive and parasitology researchers. Experimental investigations have utilized behavioral, morphological, and neuro-immune approaches in the study of experimental cutaneous leishmaniasis (CL). The data generated indicates new venues for the study of the pathological characteristics of nociceptive processing in this parasitic disease. Leishmania-induced pain may be easily observed in mice and rats. However, nociceptive data is more complex in human investigations, including the occurrence of painless lesions in mucocutaneous and cutaneous leishmaniasis. Data from recent decades indicate that humans can also be affected by pain-related symptoms, often distinct from the region of body infection. The molecular and cellular mechanisms underlying such variable nociceptive states in humans during the course of leishmaniasis are an active area of research. The present article reviews nociception in leishmaniasis, including in experimental models of CL and clinical reports.

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Estudo de validação comparativo entre as técnicas Elisa e RIFI para diagnosticar Leishmania sp em cães errantes apreendidos no município de Campos dos Goytacazes, Estado do Rio de Janeiro

Cited by 7 publications

References 0 publications

High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil

High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil

Molecular and parasitological detection of Leishmania spp. in dogs caught in Palmas, TO, Brazil

Leishmania infection: painful or painless?

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