Ethanol Extract of Stylissa carteri Induces Cell Death in Parental and Paclitaxel-Resistant Cervical Cancer Cells

Hardani, Isna Nisrina; Damara, Fachreza Aryo; Nugrahani, Annisa Dewi; Bashari, Muhammad Hasan

doi:10.15850/ijihs.v6n2.1378

Cited by 5 publications

(6 citation statements)

References 10 publications

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“…A previous study showed that the ethanol extract of Stylissa carteri was cytotoxic against cervical cancer cells (the IC 50 =1 μg/ml). 21 The findings above indicated that Stylissa carteri consisted of active compounds that could be potentially developed into potential candidates to reduce viability of some types of cancer.…”

Section: Discussionmentioning

confidence: 96%

“…19,20 The ethanol extract of Stylissa carteri has cytotoxic effects on several cancer cell lines, including luminal A, HER2 + and triple-negative type breast cancer cells, and cervical cancer cells. 21,22 To further purify compounds of Stylissa carteri and to obtain stronger potential of anticancer fraction, this study was conducted to explore effects on fraction levels, especially on the ethyl acetate fraction. This study aimed to determine the cytotoxic effects of ethyl acetate fraction of the Stylissa carteri on trastuzumabresistance HER2+ breast cancer cells, HCC-1954 in vitro through its cytotoxicity parameters using the 3-[4,5-dimethylthiazol-2-yl] -2.5 diphenyl tetrazolium bromide (MTT) assay and estimation of 50% inhibitory concentration (IC 50 ).…”

Section: Introductionmentioning

confidence: 99%

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The ethyl acetate fraction of sponge Stylissa carteri decreases viability in HER2+ trastuzumab-resistant breast cancer cells

Jannah

Fianza

Putri

et al. 2019

JKKI

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Background: HER2+ breast cancer is a very aggressive type of breast cancer. Although trastuzumab, specifically targeted for HER2, has been used for breast cancer treatments, some patients become resistant to trastuzumab. A marine sponge is one of the potential sources of anticancer agents. One of marine sponges commonly found in Indonesia is Stylissa carteri, but it has not been explored extensively. Objective: This study aimed to identify cytotoxic effects of the ethyl acetate fraction of Stylissa carteri on HCC-1954, HER2+ trastuzumabresistant breast cancer cells, by assessing cell viability and determining IC 50 value. Methods: This study was an experimental in vitro study conducted in The Cell Culture Laboratory, Faculty of Medicine Universitas Padjadjaran on February 2018 to June 2018. The Stylissa carteri was collected from Pramuka Island, Kepulauan Seribu National Park Jakarta. HCC-1954 cells were treated by serial concentration of fractions and were incubated for 72 hours. Cell viability were observed under a microscope and analysed with MTT assay. The IC 50 value was also determined by using four parametric logistic regression (4PL) method by Sigmaplot version 12.0. Result: Data of this study showed descent of cell viability significantly when exposing the ethyl acetate fraction of Stylissa carteri. There was a decrease of 49% cell viability in 10 μg/ml of the ethyl acetate fraction of Stylissa carteri. The estimated IC 50 value was 9.25 μg/ml. Conclusion: This result indicated that the ethyl acetate fraction of Stylissa carteri has cytotoxic effects on HER2+ trastuzumab-resistant breast cancer cells.Latar Belakang: Kanker payudara tipe HER2+ adalah jenis yang sangat agresif. Walaupun tersedia trastuzumab yang secara spesifik mentarget HER2, sebagian pasien mengalami resistensi terhadap trastuzumab. Spons laut diketahui berpotensi baik sebagai sumber obat anti kanker. Stylissa carteri adalah salah satu spesies spons laut yang banyak ditemukan di perairan Indonesia namun belum banyak dieksplorasi potensinya. Tujuan: Penelitian ini bermaksud untuk mengetahui efek sitotoksik fraksi etil asetat Stylissa carteri terhadap sel kanker payudara HER2+ resisten trastuzumab (HCC-1954) melalui parameter viabilitas sel dan penghitungan nilai IC 50 .

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The ethyl acetate fraction of sponge Stylissa carteri decreases viability in HER2+ trastuzumab-resistant breast cancer cells

Jannah

Fianza

Putri

et al. 2019

JKKI

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“…In addition, a recent study shows that the methanol crude extract of S. carteri has cytotoxic effects in cervical cancer HeLa cells (Dewi, 2017). Our previous data demonstrates that the Et(OH) extract of S. carteri triggers cell death in both parental HeLa cells and paclitaxel resistance HeLa cells (Hardani et al, 2018).…”

Section: Discussionmentioning

confidence: 98%

“…Species were visually identified in the field, and confirmed at Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University. Samples were cut into small size and then extracted using maceration technic in ethanol according to previous study (Hardani et al, 2018).…”

Section: Extraction Of Stylissa Carterimentioning

confidence: 99%

Bioactive Compounds in the Ethanol Extract of Marine Sponge Stylissa carteri Demonstrates Potential Anti-Cancer Activity in Breast Cancer Cells

Bashari

Huda

Tartila

et al. 2019

Asian Pac J Cancer Prev

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Objective: Despite advanced treatment options available, drug resistance develops in breast cancer (BC) patients requiring novel effective drugs. Stylissa carteri, a marine sponge predominantly living in Indonesia territories, has not been extensively studied as anti-cancer. Therefore, this study targeted to assess the anti-tumor activity of the ethanol extract of S. carteri in BC cells. Methods: S. carteri was collected from Pramuka Island, at Kepulauan Seribu National Park, Jakarta, Indonesia and extracted using ethanol. Different BC cells including MDA MB 231, MDA MB 468, SKBR3, HCC-1954 and MCF-7 cells were treated with this extract for cytotoxic analysis using MTT assay. Spheroid growth assay and apoptosis assay were conducted in HCC-1954 cells. In addition, cell migration analysis and synergistic activity with doxorubicin or paclitaxel were conducted in MDA MB 231 cells. This extract was subjected also for GC-MS analysis. Results: The results show that ethanol extract of S. carteri demonstrated a cytotoxic activity in BC cells. The IC 50 of this extract was lower 15 μg/ml in MDA MB 231, MDA MB 468, SKBR3, and HCC-1954 cells. Moreover, this extract inhibited spheroids growth and induced apoptosis in HCC-1954 cells. It inhibited cell migration and demonstrated a synergistic activity with doxorubicin or paclitaxel on triggering cell death in MDA MB 231 cells. Furthermore, GC-MS analysis indicated that this extract contained 1,2-Benzenediol, Dibutyl phthalate and 9,12-Octadecadienoic acid, ethyl ester. Conclusion: Our preliminary data indicate a potential anti-tumor activity of ethanol extract of S. carteri in breast cancer cells.

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“…The ethyl acetate fraction from ethanol extract Stylissa carteri was able to inhibit the activity of proteasome E2 (Ubc13) and the interactions between p53-MDM2 (E3) (Afifi, 2017). Our previous study showed the ethanol extract of Stylissa carteri has a cytotoxic effect in different BC cell lines including luminal A (MCF-7), HER2+ (SKBR3, HCC-1954) and TNBC (MDA MB 231), and also having an effect in cervical cancer cells (Hardani et al, 2018;Bashari et al, 2019). Moreover, it inhibits MDA MB 231 cell proliferation and migration (Bashari et al, 2019).…”

Section: Research Articlementioning

confidence: 99%

The Ethyl Acetate Fraction of Marine Sponge Stylissa carteri Induces Breast Cancer Cell Death via Upregulation of Mcl-1S: an In vitro Study

Bashari

Fadhil

Aulia

et al. 2022

Asian Pac J Cancer Prev

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Objective: To evaluate the potency of the fraction of marine sponge Stylissa carteri in inducing cell death, inhibiting spheroid growth, and its impact on pro-apoptotic protein Mcl-1S in breast cancer cells. Methods: Stylissa carteri were collected from Pramuka Island followed by ethanol extraction and ethyl acetate fractionation. To evaluate the cytotoxic effect of fraction, the HCC-1954, MDA MB 231, and MCF-7 cells were treated with the fraction of Stylissa carteri and MTT assay was then performed. The effect on spheroid growth was evaluated in HCC-1954 cells. The combined effect of the ethyl acetate fraction and paclitaxel were analyzed using combination index (CI) and immunoblotting on the pro-apoptotic protein Mcl-1S. Furthermore, compounds in this fraction were identified using GC-MS. Results: Data showed that both the MDA MB 231 and HCC-1954 cells were interestingly more sensitive to the fraction as compared with MCF-7 cells. The IC 50 of the ethyl acetate fraction on HCC-1954, MDA MB 231 and MCF-7 were 4.1 µg/ml, 3.9 µg/ml, and 123.8 µg/ml, respectively. In addition, the fraction triggered spheroid destruction within 10 days. The CI of paclitaxel and ethyl acetate fraction of Stylissa carteri were less than 0.52. Moreover, this combination induced upregulation of the Mcl-1S protein. Furthermore, some fatty acid-based structures were predicted as the major compounds in this fraction. Conclusion: The ethyl acetate fraction of Stylissa carteri induces cell death and spheroid destruction in aggressive breast cancer cells. It has a synergistic cytotoxic effect with paclitaxel on MDA MB 231 cell death and upregulates Mcl-1S protein.

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Ethanol Extract of Stylissa carteri Induces Cell Death in Parental and Paclitaxel-Resistant Cervical Cancer Cells

Cited by 5 publications

References 10 publications

The ethyl acetate fraction of sponge Stylissa carteri decreases viability in HER2+ trastuzumab-resistant breast cancer cells

The ethyl acetate fraction of sponge Stylissa carteri decreases viability in HER2+ trastuzumab-resistant breast cancer cells

Bioactive Compounds in the Ethanol Extract of Marine Sponge Stylissa carteri Demonstrates Potential Anti-Cancer Activity in Breast Cancer Cells

The Ethyl Acetate Fraction of Marine Sponge Stylissa carteri Induces Breast Cancer Cell Death via Upregulation of Mcl-1S: an In vitro Study

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