Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

Behera, Shuvashish; Mohanty, R. C.; Ray, Ramesh C.

doi:10.2478/s11756-010-0041-7

Cited by 17 publications

(19 citation statements)

References 31 publications

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“…The formation of these products is due to the fact that bacteria do not follow the glycolytic pathway, like yeasts (Ernandes and Garcia-Cruz, 2011). In studies conducted with Z. mobilis, some advantages were observed when the aim is industrial ethanol production, highlighting high specific yields; tolerance to high ethanol concentrations; ability to grow in complete anaerobiosis, unlike yeast which requires some oxygen, especially for biosynthesis of unsaturated fatty acids (Rogers et al, 2007;Behera et al, 2010b;Bochner et al, 2010). Although, Z. mobilis shows good potential for use in ethanol fermentation, it has some limitations with respect to its fermentation capacity.…”

Section: Introductionmentioning

confidence: 99%

Immobilization and association of microorganisms to improve fermentation performance for ethanol production

Almeida¹,

Angelis²

2016

J. Agric. Biotech. Sustain. Dev.

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Alcohol production in industry is carried out almost exclusively by means of batch or continuous fermentation processes. In both cases, cells are found spread throughout the fermenting substrate and have to go through a centrifugation cycle in order to be recovered. Investment in equipment is very high and performance is often lower than expected. According to available information, ethanol fermentation performance could be better in conditions where the cells are immobilized in continuous processes. In this experiment, it was possible to affirm, based on statistical data that fermentation with immobilized cells improves alcohol yields. Using a free cell batch fermentation process, Zymomonas mobilis reached 59.95% of the theoretical yield. Immobilized cells reached 68.53% using a batch and 74.49% using a continuous fermentation process. Under the same conditions, Saccharomyces cerevisiae reached respectively 70.03, 77.10 and 78.47% of the theoretical yield. Higher yields were achieved for both microorganisms using mixed culture fermentation, compared to pure cultures. Under the same conditions for both pure cultures, mixed cultures reached respectively 70.86, 79.07 and 80.86% of the theoretical yield. Findings suggest that association and immobilization cultures of S. cerevisiae and Z. mobilis result in better yields for batch as well as continuous fermentation processes.

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Section: Introductionmentioning

confidence: 99%

Immobilization and association of microorganisms to improve fermentation performance for ethanol production

Almeida¹,

Angelis²

2016

J. Agric. Biotech. Sustain. Dev.

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“…Although each method has distinct advantages and disadvantages, the most suitable approach for cell immobilization may be cell entrapment because of its simple preparation, cost-effectiveness, and high cell viability and activity (Behera et al, 2010a;Nikolić et al, 2010). The use of natural and synthetic carriers for entrapped cells has been widely studied (Phisalaphong et al, 2007;Yu et al, 2007;Zhang et al, 2007; Abstract: The aim of this investigation was to optimize immobilization conditions to entrap Kluyveromyces marxianus DBKKUY-103 during ethanol fermentation from sweet sorghum juice using a response surface methodology.…”

Section: Introductionmentioning

confidence: 99%

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Nonthasen¹,

Piyatheerawong²,

Thanonkeo³

2015

Turk J Biol

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The aim of this investigation was to optimize immobilization conditions to entrap Kluyveromyces marxianus DBKKUY-103 during ethanol fermentation from sweet sorghum juice using a response surface methodology. The effects and interactions of variables involved in cell entrapment using polyvinyl alcohol and sodium alginate were studied through a fractional factorial design. The results suggested that the primary variables involved in cell entrapment that significantly affected ethanol fermentation were sodium sulfate concentration, polyvinyl alcohol, and sodium alginate contents (P < 0.01). Subsequently, these variables were optimized for the preparation of cell entrapment based on response surface methodology and using central composite design. The results indicate that cell entrapment can achieve an ethanol concentration of 81.56 g L -1 under the following optimal conditions: bead gel diameter of 4.0 mm, calcium chloride concentration of 0.3 M, polyvinyl alcohol content of 10.09% (w v -1 ), sodium alginate content of 3.39% (w v -1 ), and sodium sulfate concentration of 0.44 M. Furthermore, statistical analysis of the model reveals that it adequately fit the experimental data (P < 0.01, R 2 = 0.9879). Therefore, this model can be used to efficiently prepare entrapped cells for ethanol production.

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“…Bio-ethanol can be produced using either free or immobilized cells (Carvalho et al 2002). Procedures for ethanol production involving the immobilization of whole cells provide several advantages: (1) the cell mass can be easily separated from the bulk liquid for possible reuse; (2) the system can function continuously over a prolonged period; (3) reactor productivity is enhanced; (4) catalytic efficiency is higher (Behera et al 2010b). Different immobilization techniques, such as entrapment in Caalginate beads, agar-agar, k-carrageenan, among others, have been used extensively in fermentation industries for producing various bio-products, such as amino acids (Bodalo et al 1996), enzymes (Kar et al 2009), organic acids (John et al 2007), and bio-ethanol (Behera et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the Gram-negative anaerobic bacterium Zymomonas mobilis has also been applied in procedures involving ethanol fermentation (Behera et al 2010b). This microorganism converts sugar almost stoichiometrically to ethanol and CO 2 , growing more rapidly and demonstrating a higher ethanol productivity than S. cerevisiae (Queresi and Manderson 1995;Rogers et al 1997;Davis et al 2005;Davis et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Ethanol production by free and immobilized cells of Z. mobilis has been studied using several substrates, i.e., molasses (Panesar et al 2001), paper sludge (Yamashita et al 2008), cassava chips and flour (Nellaiah and Gunasekaran 1992), and mahula flowers (Behera et al 2010b). In the latter study, Z. mobilis MTCC 92 entrapped in Ca-alginate matrix was studied for its potential for ethanol production and the results compared with that of free cells (Behera et al 2010b).…”

Section: Introductionmentioning

confidence: 99%

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Ethanol production from mahula (Madhuca latifolia L.) flowers using free and immobilized (in Luffa cylindrical L. sponge discs) cells of Zymomonas mobilis MTCC 92

2011

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A novel immobilization method involving Zymomonas mobilis immobilized in a luffa (Luffa cylindrica L.) matrix for ethanol production from mahula (Madhuca latifolia L.) flowers was investigated. The dried spongy fruits of luffa, a cucurbitaceous crop available in abundance in tropical and sub-tropical countries, have been found to be a promising material for immobilizing microbial cells. In our study, the cells not only survived, but they were also active physiologically for four growth cycles, yielding ethanol at 251.1±0.012, 247.9±0.08, 243.4±0.042 and 240.71±0.033 g/kg flowers in the first, second, third, and fourth cycles, respectively, after 96 h fermentation. Ethanol production by the immobilized cells was 9.2% higher than that by free cells. The ethanol yield (Yp/s), volumetric substrate uptake (Qs), and final sugar to ethanol conversion rate (%) obtained with luffa-immobilized cells of Z. mobilis were 0.439±0.004 g/g, 0.994±0.009 g/l/h, and 87.8%, respectively, which were 7.06, 2.62, and 6.92% higher than that of free cells.

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Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

Cited by 17 publications

References 31 publications

Immobilization and association of microorganisms to improve fermentation performance for ethanol production

Immobilization and association of microorganisms to improve fermentation performance for ethanol production

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Ethanol production from mahula (Madhuca latifolia L.) flowers using free and immobilized (in Luffa cylindrical L. sponge discs) cells of Zymomonas mobilis MTCC 92

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