Ethanol induces FOS immunoreactivity in the rat brain

Chang, SL; Patel, NA; Romero, AA; Kenig, V

doi:10.1016/0167-0115(94)90384-0

Cited by 6 publications

(2 citation statements)

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“…Examination of immediate early gene (I EG) expression (e.g., c-fos and NGFI-B mRNAs) has been widely used as a marker of neuronal activation (Sagar et al, 1988;Dragunow and Faull, 1989;Watson and Milbrandt, 1989;Doucet et al, 1990;Morgan and Curran, 1991;Hoffman et al, 1993). Investigation of Fos and/or NGFI-B mRNAs has been valuable in the study of PVN activation by a variety of stimuli e.g., restraint or foot shock stress (Imaki et al, 1992;Rivest and Rivier, 1994), immune challenge (Rivest et al, 1992;Chan et al, 1993;Day and Akil, 1996), and alcohol intoxication (Chang et al, 1994;Zoeller and Fletcher, 1994;Rivier and Lee, 1995;Ryabinin et al, 1997). Messenger RNAs for both c-fos and NGFI-B are induced by activation of extracellular receptors followed by translation to their respective proteins, which bind to consensus sequences on target DNAs, regulating ensuing gene expression.…”

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confidence: 99%

Divergence in the Expression of Molecular Markers of Neuronal Activation in the Parvocellular Paraventricular Nucleus of the Hypothalamus Evoked by Alcohol Administration via Different Routes

1998

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Immediate early gene (IEG) expression has been routinely used by neuroscientists as an index of neuronal activation. In the case of the hypothalamic-pituitary-adrenal axis, induction of c-fos and/or NGFI-B mRNAs in the parvocellular paraventricular nucleus (pPVN) has been documented after a variety of stimuli that increase adrenocorticotropin (ACTH) in the systemic circulation. However, the functional relationship between expression of IEGs and transcription of the genes for the ACTH secretagogues corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) is not clear. While investigating the neuroendocrine correlates of alcohol administration via different routes (intraperitoneal vs intragastric), we noted a difference in the time course of NGFI-B mRNA expression in the pPVN, despite comparable dynamics in ACTH secretion. By comparing the temporal cascade of transcriptional events in vivo after alcohol injection via either route, we sought to determine functional relationships between IEGs and the induction of CRF and AVP heteronuclear RNAs (hnRNAs). One advantage of our paradigm is the use of the same stimulus (systemic alcohol injection) in which access to the CNS does not differ between the groups to be compared. Intraperitoneal administration of the drug resulted in significant increases in c-fos mRNA, Fos protein, CRF hnRNA, and AVP hnRNA. In contrast, intragastric treatment evoked a brief, modest elevation in c-fos mRNA and Fos protein, increased AVP hnRNA, and caused no detectable change in CRF hnRNA. These data indicate that robust increases in CRF hnRNA are closely linked to full expression of c-fos mRNA and Fos protein. In addition, the expression of NGFI-B after both routes of administration is indicative of cellular activation within the pPVN in parallel with secretion of ACTH.

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confidence: 99%

Divergence in the Expression of Molecular Markers of Neuronal Activation in the Parvocellular Paraventricular Nucleus of the Hypothalamus Evoked by Alcohol Administration via Different Routes

1998

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“…CREB has also been implicated in activation of a number of immediate early genes such as c-fos (Walton and Rehfuss 1990). Acute doses of ethanol (3 and 5 g/kg) increase c-fos mRNA expression and fos protein levels in several rat brain regions (Chang et al 1994;Thiriet et al 2000), suggesting that the activation of fos expression provides a mechanism for subsequent gene regulation. Therefore, the activation of cAMP/PKA signaling by acute ethanol and increases in CRE-mediated gene expression (including immediate early genes) may lead to changes in neuronal plasticity, which may contribute to the processes of reward and addiction.…”

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confidence: 98%

Ethanol-induced Changes in Proenkephalin mRNA Expression in the Rat Nigrostriatal Pathway

2008

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Endogenous opioid systems have been suggested to play a key role in ethanol reinforcement mechanisms and alcohol-drinking behavior. Ethanol induces differential alterations in opioid peptide expression in brain areas of the reward circuits, which may be linked to the reinforcing effects of ethanol. In addition, ethanol-induced alterations in opioidergic nigrostriatal transmission could be involved in brain sensitivity to ethanol and play a role in addictive processes. The aim of this work was to study the effects of acute ethanol administration on proenkephalin (proenk) mRNA expression in the rat substantia nigra and caudate-putamen (CP) for up to 24 h post treatment. Male Wistar rats received ethanol (2.5 g/kg) or distilled water by intragastric administration, and proenk mRNA expression was studied by in situ hybridization and densitometry. Ethanol transiently increased proenk mRNA expression in the CP 1 h after drug administration. Proenk mRNA levels remained elevated 2 h post treatment in the anterior-medial and medial-posterior regions of the CP. In contrast, ethanol decreased proenk mRNA expression in the substantia nigra pars compacta and pars reticulata 2 h after drug exposure. Alterations in enkephalin expression in the substantia nigra and CP in response to ethanol exposure could be involved in the mechanisms underlying brain sensitivity to the drug.

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Acute alcohol administration stimulates the activity of hypothalamic neurons that express corticotropin-releasing factor and vasopressin

1996

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Ethanol induces FOS immunoreactivity in the rat brain

Cited by 6 publications

References 2 publications

Divergence in the Expression of Molecular Markers of Neuronal Activation in the Parvocellular Paraventricular Nucleus of the Hypothalamus Evoked by Alcohol Administration via Different Routes

Divergence in the Expression of Molecular Markers of Neuronal Activation in the Parvocellular Paraventricular Nucleus of the Hypothalamus Evoked by Alcohol Administration via Different Routes

Ethanol-induced Changes in Proenkephalin mRNA Expression in the Rat Nigrostriatal Pathway

Acute alcohol administration stimulates the activity of hypothalamic neurons that express corticotropin-releasing factor and vasopressin

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