Ethanol production from rice straw using thermotolerant Kluyveromyces sp. IIPE453

Jain, Rakesh; Ghosh, Debashish; Agrawal, Deepti; Suman, Shankar; Pandey, Dhirendra K.; Vadde, Vasantha Thakur; Dixit, Ashwini Kumar; Adhikari, Dilip K.; Dasgupta, Diptarka

doi:10.1007/s13399-014-0143-5

Cited by 19 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ethanol production by K. marxianus DBTIOC-35 during SSF has been compared with previous studies in Table 3. As can be seen, maximum overall ethanol productivity of 0.92 g L À1 h À1 obtained in this study at 20% biomass loading was better than 0.39 g L À1 h À1 obtained by both Mohagheghi et al 21 and Bollók et al, 33 and 0.885 g L À1 h À1 reported by Jain et al 34 Though there are some previous reports on SSF without pre-saccharication step, simple SSF process i.e. without any presaccharication or any other modications (such as delayed temperature SSF or nonisothermal SSF) have not resulted in such high ethanol titres of 66.2 g L À1 (ethanol yield of 83.3%) as have been obtained in this study under high gravity SSF of pilot scale dilute acid pretreated wheat straw.…”

Section: Comparison Of Shf Ssf and Pssfcontrasting

confidence: 38%

Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Comparison Of Shf Ssf and Pssfcontrasting

confidence: 38%

Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Estimates have shown generation of 1.35 tons of rice straw annually for every ton of harvested grain (Kadam et al 2000). Characteristics of the rice straw, such as low bulk density, high mineral, and silica contents, limit its applications (Jain et al 2015). Its utilization as animal fodder is also unattractive because of its low digestibility, low protein content, high lignin, and silica contents (Kausar et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…It has been seen that burning rice straw in open fields is a common practice all over the world, which leads to air pollution (Gadde et al 2009;Emtenan et al 2012;Singh et al 2016). An alternative to this is using rice straw as a feedstock for production of cellulosic ethanol (Park et al 2011;Jain et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01

et al. 2017

View full text Add to dashboard Cite

Alkali-assisted acid pretreated rice straw was saccharified using cellulase from Aspergillus niger BK01. The cellulase production by the fungus was enhanced by parametric optimization using solid-state fermentation conditions. Maximum cellulase production (12.0 U/gds of carboxymethyl cellulase, CMCase) was achieved in 96 h, using 6.0% substrate concentration, 7.5% inoculum concentration, 1:2 solid to liquid ratio, at pH 5.5, and temperature 28°C, by supplementation of the fermentation medium with 0.1% carboxymethylcellulose and 0.1% ammonium nitrate. Characterization of crude cellulases showed that highest CMCase activity was observed at pH 4.8 and temperature 40°C. The CMCase was stable from pH 4.8-5.5 and at a temperature range of 35-50°C. The pretreated biomass was subjected to hydrolysis with the fungal cellulases. The saccharification optimization studies showed that 2% (v/v) enzyme concentration and hydrolysis time of 2.5 h were optimum for maximum yield, i.e, 23.78% sugars and 35.96% saccharification value.

show abstract

“…These no‐food raw materials include various types of plant biomass also known as LC biomass (Robak & Balcerek, 2018). Among various LC biomass substrates, RS is preferred for ethanol production due to its high polysaccharide and low lignin contents (Jain et al, 2015). Therefore, we assessed the ethanol production potential of the selected yeast strains from alkali‐treated RS through SHF and SSF approaches at higher temperatures.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting thermotolerant yeasts from distillery effluent and molasses for high‐temperature ethanol production

Avchar

Lanjekar

Baghela

2021

J of Applied Microbiology

View full text Add to dashboard Cite

Aims: Isolation, characterization and assessment of inhibitor tolerance of thermotolerant yeasts associated with distillery effluent and molasses, and their use in hightemperature ethanol production from alkali-treated rice straw. Methods and Results:A total of 92 thermotolerant yeasts were isolated from seven different distillery effluent and molasses samples. Based on MSP-PCR, 34 yeasts were selected and identified by sequencing the D1/D2 domain of LSU rDNA. These yeasts belonged to eight genera and nine different species. We assessed the inhibitor tolerance of these 34 well-characterized yeasts against various pre-treatment-generated inhibitors (furfural, 5-hydroxymethyl furfural and acetic acid) and also evaluated their ethanol yields at 40, 45 and 50℃. Among selected strains, Pichia kudriavzevii DSA3.2 exhibited the highest ethanol production (24.5 g l −1 ) with an efficiency of 95.7% at 40℃ using 5% glucose. At 45℃, P. kudriavzevii DSA3.2 and Kluyveromyces marxianus MSS6.3 yielded maximum ethanol titres; 22.3 and 23 g l −1 with 87.4% and 90% efficiency, respectively. While using alkali-treated RS at 45℃, K. marxianus MSS6.3 produced 10.5 g l −1 of ethanol with 84.5% fermentation efficiency via separate hydrolysis and fermentation, and 10.9 g l −1 of ethanol with 85% efficiency via simultaneous saccharification and fermentation. Pichia kudriavzevii DSA3.2, DSA3.1 and K. marxianus MSS6.3 also exhibited significant tolerance against multiple inhibitors. Conclusions: Yeast isolates P. kudriavzevii DSA3.2 and K. marxianus MSS6.3 exhibited significant inhibitor tolerance and proved to be suitable for high-temperature ethanol fermentation. After additional optimization and scale-up experiments, these isolates can be exemplary candidates for industrial-scale ethanol production from lignocellulosic biomass. Significance and Impact of the Study:Our study recognizes distillery effluents and molasses as specialized niches for yeasts with a broad substrate range, capable of tolerating multiple inhibitors and yielding high levels of ethanol at elevated temperatures. These yeasts can further be exploited for bioethanol production through SSF/SHF at a larger scale.

show abstract

Ethanol production from rice straw using thermotolerant Kluyveromyces sp. IIPE453

Cited by 19 publications

References 37 publications

Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01

Bioprospecting thermotolerant yeasts from distillery effluent and molasses for high‐temperature ethanol production

Contact Info

Product

Resources

About