Ethanol toxicity and cardiac protein synthesis in vivo

Preedy, Victor R.; Siddiq, Tahir; Why, Howard; Richardson, Peter J.

doi:10.1016/0002-8703(94)90072-8

Cited by 32 publications

(12 citation statements)

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“…The use of the technique for investigating the genesis of alcoholic cardiomyopathy is handicapped by the ethics involved in biopsying patients in which the cause of the disease entity is known, hence the rationale of conducting an investigation into alcoholic cardiomyopathy in an animal model. Features of ACM include myofibrillar architecture damage, thus resulting in defects in heart contractility [29] and similar features occur in the rat model [ 6 ] . aortic stenosis, showed cardiac protein patterns displaying marked decreases in proteins ranging from M, 35000-41000 when compared to normal controls [26].…”

Section: Application Of 2-de To Other Heart Pathologiesmentioning

confidence: 76%

“…The pathogenic mechanisms responsible for ACM are generally unknown, although decreases in either cardiac protein synthesis, formation of acetaldehyde protein adducts, altered mitochondria1 function resulting in reduced contractility and the generation of reactive oxygen species may be precipitating events [4,5]. Preedy et al [6] have recently indicated that an important feature of experimental ethanol dosage studies are changes in protein turnover. In chronic feeding experiments studies there was also a small reduction in the myofibrillar proteins which may be of functional importance [7].…”

Section: Introductionmentioning

confidence: 99%

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Protein profiling in cardiac tissue in response to the chronic effects of alcohol

et al. 1997

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An investigation was made into cardiac protein levels after chronic ethanol consumption to examine whether specific proteins are affected by alcohol. Ethanol was administered for six weeks to male Wistar rats which were fed a nutritionally complete liquid diet containing 35% of total calories as ethanol. Controls were pair-fed identical amounts of the same diet in which ethanol was replaced by isocaloric glucose; thus both groups had identical nutritional intakes, albeit differences in ethanol or carbohydrate. After six weeks' feeding, cardiac tissue was removed and analyzed by two-dimensional electrophoresis, where equal amounts of proteins were studied. Protein patterns were analyzed by computerized densitometry and characterized by comparison with a database of known cardiac proteins. Chronic alcohol feeding caused significant decreases in the relative amounts of various proteins, including several tentatively identified as heat shock protein (HSP) 60, HSP70, and desmin. The relative proportions of actin, vimentin, myosin light chain 1, myosin light chain 2, and albumin, remained unchanged. Examination of antibodies raised against HSP65 showed no overt differences in plasma levels following chronic alcohol consumption, and liver changes as assessed by histology were mild. In conclusion, chronic alcohol appears to have selective effects on particular proteins, and the effects were not directly ascribed to overt liver dysfunction or malnutrition. This may explain some of the functional and morphological characteristics observed in alcohol-induced heart muscle disease, including reduced contractility.

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Section: Application Of 2-de To Other Heart Pathologiesmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Protein profiling in cardiac tissue in response to the chronic effects of alcohol

et al. 1997

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“…Various mechanisms have been proposed to account for the changes in the heart muscle in alcoholism, including changes in the synthesis of contractile proteins (25,(42)(43)(44)(45) and the induction of autoantibodies due to the formation of acetaldehyde-protein adducts (46). The involvement of lipid peroxidation and hence free radical mediated damage has also been hypothesized in the pathogenesis of ACM.…”

Section: Clinical Features Of Alcoholic Cardiomyopathymentioning

confidence: 99%

Oxidants antioxidants and alcohol implications for skeletal and cardiac muscle

Preedy¹

1999

Front Biosci

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“…However, it is common knowledge that alcohol consumption has not only beneficial, but also adverse effects. Consumption of alcoholic beverages can have a negative influence on protein metabolism [7,8]. These negative changes also include plasma circulating proteins [9].…”

Section: Introductionmentioning

confidence: 99%