Ethnobotanical study, antifungal activity, phytochemical screening and total phenolic content of Algerian Aristolochia longa

Abstract

Aim:Aristolochia longa (from the family Aristolochiaceae) is widely used in Algerian traditional medicine. Here, we document ethnomedicinal uses by local population of Mascara province (West Algeria) and we evaluate the antifungal activity, the phytochemical composition and total phenolic content of aqueous extract (decoction) of the roots of A. longa from Algeria.Materials and Methods:The ethnobotanical investigation was carried out in Mascara Province (West Algeria). Antifungal activity was assessed against Saccharomyces cerevisiae. Total phenolic content was measured using the Folin–Ciocalteu’s reagent.Results:Our results showed that A. longa is widely used to treat several ailments such as cancer (38%), skin infections (14%), and diabetes (11%). Crushed roots are commonly used (89%) mixed with honey, milk, water or other medicinal plants. A. longa aqueous extract induced growth inhibition of S. cerevisiae cells in a dose - and time - dependent manner. An effective suppression of S. cerevisiae (97.06% inhibition of proliferation) was obtained at the 500 µg/mL after 72 h. Results of the phytochemical screening revealed that A. longa aqueous extract contained various bioactive compounds, including polyphenols and flavonoids. Total phenolic content in A. longa aqueous extract was found to be 6.07 ± 0.12 mg (gallic acid equivalents)/g.Conclusion:A. longa may be considered as a promising source of new drugs for treating cancer and as a good antifungal agent.

An investigation of chemical content, enzyme inhibitory propert, antioxidant and antibacterial activity of Aristolochia bodamae Dingler (develiotu) (Aristolochiaceae) root extracts from Samsun, Turkey

“…In the same study, phenolic content of A indica root extract was determined as 54.27 ± 0.77 mg GAE/g extract . For the aqueous extract, the total phenolic content of A longa of was found to be 6.07 ± 0.12 mg GAE/g dry plant, for methanol extract, 132.33 ± 3.77 μg GAE/mg dry plant, and for acetone extract 525.43 ± 29.6 μg GAE/mg dry plant…”

“…found to be more effective than ABRME (EC 50 : 130.76 µg/mL). 12,47 Besides, Benmehdi and Behilil reported that activity of A clematitis root methanol extract was 0.142 mg/mL. 23 Das et al 54 The source of the diseases caused by oxidative stress is the free radicals formed by transition metal ions in living systems.…”

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“…Previously, Djeridane et al, reported the following results from roots methanol extract of Aristolochia longa L. 4 : total phenolics 1.47±0.02 mg GAE/g dw, flavonoids content 0.81±0.02 mg RE/g dw, flavonols content 0.41±0.002 mg QE/g dw, and also in other studies 33 , total phenolic content in A. longa aqueous extract of roots was found to be 6.07 ± 0.12 mg GAE/g, but our extract contain higher levels, this could be explained by the change of the plot location.…”

A Study on the Bioefficacy of Caryota Urens L.

“…The amount of oxalic acid in leaves of C. urens per 100 g was estimated using the regression equation ΔA = 0.966C -0.027, where C is the concentration of oxalic acid in mg/ml and ΔA is Ab-As (Table 2﴿. www.wjpr.net Phenolic compounds have redox properties, which allow them to act as antioxidants, as their free radical scavenging ability is facilitated by their hydroxyl groups. [21] Terpenes play an important role as signal and growth regulators of plants. Since, terpenoids promote glutathione s-transferase and cell apoptosis, they have been used for their anti-cancer properties.…”

“…The activity of C. urens could be attributed due to presence of terpenoids, phenols and saponins. [30] [31] The mechanism of inhibition by terpenoids involves membrane disruption by the lipophilic compounds. Phenols are effective against bacteria and fungi because they inhibit enzymes by the oxidized compounds possibly through reactions with sulfhydryl group or through more non-specific interactions with proteins.…”