Ethyl acetate extract from Selaginella doederleinii Hieron inhibits the growth of human lung cancer cells A549 via caspase-dependent apoptosis pathway

Sui, Yuxia; Li, Shaoguang; Shi, Peiying; Wu, Youjia; Li, Yuxiang; Chen, Wei‐Ying; Huang, Liying; Yao, Hong; Lin, Xinhua

doi:10.1016/j.jep.2016.06.029

Cited by 54 publications

(47 citation statements)

References 28 publications

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“…It is widely used to treat sore throats, rheumatic arthralgia pain, nasopharyngeal carcinoma, chorionic carcinoma, lung cancer, cervical cancer and a variety of diseases such as traumatic bleeding [8][9][10][11][12][13]. However, its anti-tumor effect of the material basis has reported relatively less.…”

Section: Discussionmentioning

confidence: 99%

Identification of chemical constituents of Selaginella doederleinii Hieron

Brad¹,

Zhang²,

Xu³

et al. 2017

Proceedings of the 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017)

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Abstract.To determine the chemical constituents of Selaginella doederleinii Hieron. The compounds were isolated and purified from the Ethyl Acetate Fraction from Ethanol Extract of Selaginella doederleinii Hieron by multiple chromatographic techniques including macroporous resin, polyamide, silica gel, Sephadex LH-20 and pre-HPLC. The structures were identified on the basis of thierphysico-chemical properties and spectroscopic data analyses

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Section: Discussionmentioning

confidence: 99%

Identification of chemical constituents of Selaginella doederleinii Hieron

Brad¹,

Zhang²,

Xu³

et al. 2017

Proceedings of the 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017)

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“…Modern pharmacological studies have also revealed that the ethanol extract of S. doederleinii could induce cancer cells apoptosis, significantly inhibit the tumor growth, and enhance the antitumor immune response. However, this extract did not display oral acute toxicity in vivo (Sui et al, 2016). Due to including the abundant biflavonoids, the total biflavonoids extract of S. doederleinii (TBESD) had more powerful anti-cancer activities than its ethanol extract .…”

Section: Introductionmentioning

confidence: 99%

Improved solubility, dissolution rate, and oral bioavailability of main biflavonoids from Selaginella doederleinii extract by amorphous solid dispersion

et al. 2020

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2020) Improved solubility, dissolution rate, and oral bioavailability of main biflavonoids from Selaginelladoederleinii extract by amorphous solid dispersion, Drug Delivery, 27:1, 309-322, ABSTRACT Amentoflavone, robustaflavone, 2 00 ,3 00 -dihydro-3 0 ,3 000 -biapigenin, 3 0 ,3 000 -binaringenin, and delicaflavone are five major hydrophobic components in the total biflavonoids extract from Selaginella doederleinii (TBESD) that display favorable anticancer properties. The purpose of this study was to develop a new oral delivery formulation to improve the solubilities, dissolution rates, and oral bioavailabilities of the main ingredients in TBESD by the solid dispersion technique. Solid dispersions of TBESD with various hydrophilic polymers were prepared, and different technologies were applied to select the suitable carrier and method. TBESD amorphous solid dispersion (TBESD-ASD) with polyvinylpyrrolidone K-30 was successfully prepared by the solvent evaporation method. The physicochemical properties of TBESD-ASD were investigated by scanning electron microscopy, differential scanning calorimetry, and Fourier-transform infrared spectroscopy. As a result, TBESD was found to be molecularly dispersed in the amorphous carrier. The solubilities and dissolution rates of all five ingredients in the TBESD-ASD were significantly increased (nearly 100% release), compared with raw TBESD. Meanwhile, TBESD-ASD showed good preservation stability for 3 months under accelerated conditions of 40 C and 75% relative humidity. A subsequent pharmacokinetic study in rats revealed that C max and AUC 0-t of all five components were significantly increased by the solid dispersion preparation. An in vivo study clearly revealed that compared to raw TBESD, a significant reduction in tumor size and microvascular density occurred after oral administration of TBESD-ASD to xenograft-bearing tumor mice. Collectively, the developed TBESD-ASD with the improved solubility, dissolution rates and oral bio-availabilities of the main ingredients could be a promising chemotherapeutic agent for cancer treatment. ARTICLE HISTORY

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“…a, molecular formula C30H18O10) with high stability is extracted from Selaginella tamariscina , Juniperus phoenicea , Rhus succedanea , and several other plants and is commonly used in oriental medicines . Hinokiflavone is a biflavonoid active constituent of flavonoids, a class of plant secondary metabolites, which have been reported to have multiple biological properties, including antioxidative, anti‐inflammatory, antimicrobial, and antitumor . Theodoratou et al .…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13] Hinokiflavone is a biflavonoid active constituent of flavonoids, a class of plant secondary metabolites, which have been reported to have multiple biological properties, including antioxidative, anti-inflammatory, antimicrobial, and antitumor. 12,[14][15][16][17] Theodoratou et al found strong and linear inverse associations between flavonoids and CRC risk by using data from a national prospective case-control study in Scotland. 18 Kang et al found that ochnaflavone, a natural biflavonoid, could induce cell cycle arrest and apoptosis in colon cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Antitumor activity in colorectal cancer induced by hinokiflavone

Zhou

Zhao

et al. 2019

J of Gastro and Hepatol

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Background and Aim Colorectal cancer is one of the most common malignant disease worldwide with highly metastatic potential. Identification of effective therapeutic treatment overcoming such disease is an urgent need. Our study focuses on hinokiflavone as an antitumor agent against colorectal cancer. Methods MTT assay, cell colony formation assay, Hoechst staining, flow cytometry, Western blot analysis, real‐time polymerase chain reaction, and migration and invasion assay were performed to identify the effects of hinokiflavone on cell proliferation, apoptosis, and metastasis. CT26 tumor‐bearing mice model was conducted to explore the antitumor activity of hinokiflavone in vivo. Immunohistochemistry staining was used to detect the protein expression of Ki‐67, cleaved caspase‐3, and MMP9 in treated tumors. Acute toxicity was evaluated by serological and hematological analyses, and drug side effect on organs was evaluated by hematoxylin and eosin staining. Results Hinokiflavone reduced the proliferation, migration, and invasion and promoted the apoptosis in colorectal tumor cells in vitro. Treatment of hinokiflavone at a tolerable and safe dose (50 mg/kg) significantly suppressed tumor growth in mice bearing CT26 tumors by reducing tumor proliferation and metastasis and inducing apoptosis. Mechanically, treatment of hinokiflavone induced apoptosis by loss of mitochondrial transmembrane potential and increased reactive oxygen species generation. Conclusions Hinokiflavone suppressed colorectal tumor cell proliferation, induced apoptosis via the reactive oxygen species–mitochondria‐mediated apoptotic pathway, and inhibited tumor cell migration and invasion. Antitumor activity of hinokiflavone was also validated in mice model without observed toxicity. Our findings suggested that the plant‐derived hinokiflavone could be used as an antitumor agent against colorectal cancer.

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Ethyl acetate extract from Selaginella doederleinii Hieron inhibits the growth of human lung cancer cells A549 via caspase-dependent apoptosis pathway

Cited by 54 publications

References 28 publications

Identification of chemical constituents of Selaginella doederleinii Hieron

Identification of chemical constituents of Selaginella doederleinii Hieron

Improved solubility, dissolution rate, and oral bioavailability of main biflavonoids from Selaginella doederleinii extract by amorphous solid dispersion

Antitumor activity in colorectal cancer induced by hinokiflavone

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