Ethylene insensitive and post-harvest yellowing retardation in mutant ethylene response sensor (boers) gene transformed broccoli (Brassica olercea var. italica)

Chen, Long-Fang O.; Huang, Jiayuan; Wang, Yung-Hsiang; Chen, Yuting; Shaw, Jei‐Fu

doi:10.1023/b:molb.0000047740.99884.97

Cited by 16 publications

(4 citation statements)

References 45 publications

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“…Additionally, the yellowing of leaves during senescence in these transgenic tobacco plants was delayed, in comparison to the wild type. These results suggest that both mutant genes conferred reduced ethylene sensitivity to transgenic plants, just as the expression of ethylene receptor genes in heterologous plants conferred insensitivity or reduced sensitivity to ethylene in several plant species (Bovy et al 1999;Chen et al 2004;Knoester et al 1998;Shaw et al 2002;Wang and Kumar, 2004;Wilkinson et al 1997).…”

Section: Expression Of a Mutated Ethylene Receptor Gene Affects Floramentioning

confidence: 99%

“…Thus, the addition of wild-type ethylene receptor genes should reduce ethylene responsiveness. Petunia plants transformed with a mutated allele of ERS from Brassica showed extended flower longevity (Shaw et al 2002), and broccoli plants transformed with the boers gene driven by the CaMV 35S promoter showed delayed senescence in leaves and floral heads (Chen et al 2004). However, the transformants also showed pleiotropic phenotypes, such as slower plant growth, shorter plant height, and late bolting.…”

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confidence: 99%

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Production of male sterile transgenic plants

Takada

Kamada

Ezura

2005

Plant Biotechnology

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Ethylene controls many physiological and developmental processes in plants, including fruit and flower development, reproductive physiology, and responses to environmental stimuli. Ethylene exerts its effects through the ethylene receptor of plants. Ethylene receptor genes have been isolated in a variety of plant species, and in early studies, these genes were used to genetically engineer fruit ripening in tomato and flower senescence in petunia and carnation. Recently, we demonstrated that the over-expression of mutated melon ethylene receptor genes affected pollen development and induced a male sterile phenotype in transgenic plants. One major concern regarding genetically modified plants is the transgene flow through pollen dispersal, which may pose a potential impact to the environment, especially on genetic diversity. Therefore, the inducible male sterility system using mutated ethylene receptor genes could be a possible strategy for preventing pollen dispersal from these plants, thereby reducing the potential impact associated with transgenic plants. This review summarizes the studies on the inducible male sterility system that uses ethylene receptor genes.

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Section: Expression Of a Mutated Ethylene Receptor Gene Affects Floramentioning

confidence: 99%

mentioning

confidence: 99%

Production of male sterile transgenic plants

Takada

Kamada

Ezura

2005

Plant Biotechnology

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“…Indeed, both molecules stimulated respiration and ET production as well as yellowing, measured as chlorophyll and hue-angle decline on broccoli heads ( Tian et al., 1994 ). Through directed mutagenesis, a mutant broccoli ET-response sensor ( ers ) gene was obtained, and two plasmids were constructed obtaining two transgenic lines ( Chen et al., 2004 ). Transgenic broccoli showed ET insensitivity, a delaying in leaves senescence as well as a retard of 1-2 days in head yellowing, compared to non-mutant lines ( Chen et al., 2004 ).…”

Section: Evolution Of Breeding Technologies For Postharvest Qualitymentioning

confidence: 99%

Ethylene: Management and breeding for postharvest quality in vegetable crops. A review

Natalini

2022

Front. Plant Sci.

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Ethylene is a two-carbon gaseous plant growth regulator that involved in several important physiological events, including growth, development, ripening and senescence of fruits, vegetables, and ornamental crops. The hormone accelerates ripening of ethylene sensitive fruits, leafy greens and vegetables at micromolar concentrations, and its accumulation can led to fruit decay and waste during the postharvest stage. Several strategies of crops management and techniques of plant breeding have been attempted in the last decades to understand ethylene regulation pathways and ethylene-dependent biochemical and physiological processes, with the final aim to extend the produce shelf-life and improve the postharvest quality of fruits and vegetables. These investigation approaches involve the use of conventional and new breeding techniques, including precise genome-editing. This review paper aims to provide a relevant overview on the state of the art related to the use of modern breeding techniques focused on ethylene and ethylene-related metabolism, as well as on the possible postharvest technological applications for the postharvest management of ethylene-sensitive crops. An updated view and perspective on the implications of new breeding and management strategies to maintain the quality and the marketability of different crops during postharvest are given, with particular focus on: postharvest physiology (ethylene dependent) for mature and immature fruits and vegetables; postharvest quality management of vegetables: fresh and fresh cut products, focusing on the most important ethylene-dependent biochemical pathways; evolution of breeding technologies for facing old and new challenges in postharvest quality of vegetable crops: from conventional breeding and marker assisted selection to new breeding technologies focusing on transgenesis and gene editing. Examples of applied breeding techniques for model plants (tomato, zucchini and brocccoli) are given to elucidate ethylene metabolism, as well as beneficial and detrimental ethylene effects.

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“…Transgene copy number was further confirmed by quantitative PCR (data not included). The total genomic DNA was isolated according to Chen et al (2004).…”

Section: Molecular Identification and Quantification Of Transgene Expmentioning

confidence: 99%

Growth, yield and shelf-life of isopentenyltransferase (ipt)-gene transformed broccoli

Chan¹,

Chen²,

Lu³

et al. 2009

Can. J. Plant Sci.

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. 2009. Growth, yield and shelf-life of isopentenyltransferase (ipt)-gene transformed broccoli. Can. J. Plant Sci. 89: 701Á711. Loss of chlorophyll leading to floret yellowing limits the post-harvest lifespan of broccoli (Brassica oleracea L. var. italica Plenck). Cytokinins are known to delay floral yellowing of plants. A transgene construct pSG766A, which results in the expression of isopentenyltransferase (ipt), the key enzyme for cytokinin synthesis, has been developed in broccoli. Expression of the ipt transgene is triggered by the senescence-associated gene promoter (SAG-13). Three selfed T 5 lines of ipt transformed broccoli (lines 101, 102 and 103) have been obtained through selection for single copy insertion, acceptable horticultural traits and transgene ipt activity. These three transgenic inbred lines were evaluated in the field during 2004Á 2007 to determine their growth, yield and shelf-life after harvest, relative to a non-transgenic inbred line (104) and the parental variety Green King. For most of the vegetative growth parameters measured, year-to-year variability exceeded line-to-line variability. Inbreeding had little impact on the appearance or yield potential of the broccoli lines. Head yields of the transgenic inbred lines 102 and 103 were comparable to the parental variety Green King, but were significantly higher than the non-transgenic inbred line 104, as lines 102 and 103 produced more plants with heavier flower heads. Cytokinin content in the form of isopentenyladenosine was relatively higher in the transgenic lines than in the two nontransgenic controls. When flower heads were stored at 25928C, the period required to cause 50% floret yellowing was 7.5 and 8. Plant Sci. 89: 701Á711. La perte de chlorophylle responsable du jaunissement des fleurons diminue la dure´e de conservation apre`s re´colte du brocoli (Brassica oleracea L. var. italica Plenck). On sait que les cytokinines retardent le jaunissement des fleurs chez les plantes. Par ailleurs, on a cre´e´le transge`ne pSG766A, qui exprime l'isopente´nyltransfe´rase (ipt), principal enzyme responsable de la synthe`se des cytokinines chez le brocoli. L'expression de ce ge`ne est de´clenche´e par le promoteur SAG-13, associe´a`la se´nescence. Les auteurs ont obtenu trois ligne´es T 5 autofe´conde´es de brocoli portant le transge`ne ipt (ligne´es 101, 102 et 103) en se´lectionnant les plants pour l'insertion d'une copie unique du transge`ne, des caracte`res horticoles acceptables et l'activite´du transge`ne. Les trois ligne´es autogames ont ensuite e´te´e´value´es au champ de 2004 a2 007 en vue d'en pre´ciser la croissance, le rendement et la dure´e de conservation apre`s re´colte, comparativement a`une ligne´e autogame non transge´nique (104) et a`la varie´te´parentale Green King. La variabilite´de la plupart des parame`tres de la croissance ve´ge´tative mesure´s d'une anne´e a`l'autre de´passe la variabilite´observe´e entre les ligne´es. L'autofe´condation agit peu sur l'aspect ou le rendement potentiel des ligne´es de brocoli. Le nomb...

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Ethylene insensitive and post-harvest yellowing retardation in mutant ethylene response sensor (boers) gene transformed broccoli (Brassica olercea var. italica)

Cited by 16 publications

References 45 publications

Production of male sterile transgenic plants

Production of male sterile transgenic plants

Ethylene: Management and breeding for postharvest quality in vegetable crops. A review

Growth, yield and shelf-life of isopentenyltransferase (ipt)-gene transformed broccoli

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