Eupatilin inhibits lipopolysaccharide-induced expression of inflammatory mediators in macrophages

Choi, Eun‐Ju; Lee, So‐Young; Chae, Jeong-Ryong; Lee, Hyun‐Shik; Jun, Chang‐Duk; Kim, Sang-Hyun

doi:10.1016/j.lfs.2011.04.011

Cited by 66 publications

(41 citation statements)

References 29 publications

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“…These results were in agreement with previous studies that showed that SLAK exerted an anti-inflammatory effect in J774A.1 macrophages through the inhibition of NO and PGE 2 production as well iNOS and COX-2 expression (Emami et al, 2010). Moreover, the overall impact of these changes also reflect results of previous studies that demonstrated that various individual sesquiterpene compounds from Artemisia exert anti-inflammatory effects that may be effective in treating some types of experimental inflammation/autoimmune conditions (Choi et al, 2011;Ho et al, 2014;Kim et al, 2002;Lu et al, 2009;Omer et al, 2007;Sun et al, 2010;Yang et al, 2012).…”

Section: Discussionsupporting

confidence: 92%

“…Different classes of chemicals, e.g., coumarins, terpenes, sesquiterpenes, flavonoids, aromatics, dipeptides, phenolics, esterols, germacranolides, secoguaianolides, and several polysaccharides have been isolated from Artemisia species (Valless et al, 2011). In recent years, increasing evidence has shown that sesquiterpene lactones are one of the constituents that impart a wide-range of pharmacological effects, including anti-cancer and immunomodulatory actions (Choi et al, 2011;Kim et al, 2002;Lu et al, 2009;Yang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Rabe¹,

Iranshahi

Rastin³

et al. 2014

Journal of Immunotoxicology

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Artemisia species are important medicinal plants throughout the world. The present in vitro study, using a sesquiterpene lactone-bearing fraction prepared from Artemisia khorassanica (SLAK), sought to investigate immunomodulatory/anti-inflammatory properties of this plant and elucidate potential underlying mechanisms for the actions. Effects of the SLAK on mitogeninduced murine splenocyte proliferation and interleukin (IL)-4 and interferon (IFN)-secretion were evaluated. To assess anti-inflammatory activities, levels of inducible of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ), as well as expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in peritoneal macrophages was examined. The results showed that SLAK noticeably was capable of suppressing PHA/LPS-stimulated splenocyte proliferation and of up-regulating production of the T-helper (T H )-2 cell cytokine IL-4 while down-regulating formation of T H 1 IFN. In addition, while SLAK caused negligible proliferation inhibition, peritoneal macrophages displayed considerable decrease in NO and PGE 2 production along with iNOS and COX-2 expression. The current experiment shows Artemisia khorasanica -a traditionally used herb -may have immunomodulatory and anti-inflammatory effects. It is anticipated that the ingredients may be employed as therapeutic candidates in the regulation of some immune responses implicated in various conditions and ailments.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Rabe¹,

Iranshahi

Rastin³

et al. 2014

Journal of Immunotoxicology

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“…Eupatilin (5,7-dihydroxy-3',4', 6-trimethoxyflavone) is one of the pharmacologically active components found in A. asiatica (7). Previous studies have reported that it has a variety of biological properties, including immune regulation, anti-inflammatory, anti-ulcer and anti-oxidative activity (8)(9)(10)(11). Furthermore, there is increasing evidence that eupatilin possesses anti-tumor effects.…”

Section: Introductionmentioning

confidence: 99%

Eupatilin induces human renal cancer cell apoptosis via ROS-mediated MAPK and PI3K/AKT signaling pathways

et al. 2016

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Abstract. Phosphatidylinositol 3-kinase (PI3K)/AKT and mitogen activated protein kinase (MAPK) signaling cascades have significant roles in cell proliferation, survival, angiogenesis and metastasis of tumor cells. Eupatilin, one of the major compounds present in Artemisia species, has been demonstrated to have antitumor properties. However, the effect of eupatilin in renal cell carcinoma (RCC) remains to be elucidated. Therefore, the present study investigated the biological effects and mechanisms of eupatilin in RCC cell apoptosis. The results of the present study demonstrated that eupatilin significantly induced cell apoptosis and enhanced the production of reactive oxygen species (ROS) in 786-O cells. In addition, eupatilin induced phosphorylation of p38α (Thr180/Tyr182), extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase 1/2 (Thr183/Tyr185), and decreased the phosphorylation of PI3K and AKT in 786-O cells in a concentration-dependent manner. Furthermore, the ROS inhibitor N-acetyl-L-cysteine was able to rescue the MAPK activation and PI3K/AKT inhibition induced by eupatilin. Taken together, the results of the present study provide evidence that inhibition of eupatilin induces apoptosis in human RCC via ROS-mediated activation of the MAPK signaling pathway and inhibition of the PI3K/AKT signaling pathway. Thus, eupatilin may serve as a potential therapeutic agent for the treatment of human RCC.

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“…RT-PCR was performed as previously described in related studies. 23 For quantitative PCR, real-time PCR was performed in triplicate using 12.5 lL of SYBR Premix Ex Taq (Takara, Japan) and 2 lL of cDNA as a template in a final volume of 25 lL. PCR amplification was preceded by incubation of the mixture for 15 min at 95°C and 40 cycles of the amplification step.…”

Section: Analysis Of Mrna Expressionmentioning

confidence: 99%

Artemisia asiaticaNakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways

Kim

Tang

Cha

et al. 2015

Journal of Medicinal Food

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The present study aimed to examine the anti-inflammatory effects and potential mechanism of action of Artemisia asiatica Nakai (A. asiatica Nakai) extract in activated murine macrophages. A. asiatica Nakai extract showed dosedependent suppression of lipopolysaccharide (LPS)-induced nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 activity. It also showed dose-dependent inhibition of nuclear factor-jB (NF-jB) translocation from the cytosol to the nucleus and as an inhibitor of NF-jB-alpha phosphorylation. The extract's inhibitory effects were found to be mediated through NF-jB inhibition and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 in LPS-stimulated J774A.1 murine macrophages, suggesting a potential mechanism for the anti-inflammatory activity of A. asiatica Nakai. To our knowledge, this is the first report of the anti-inflammatory effects of A. asiatica Nakai on J774A.1 murine macrophages; these results may help develop functional foods possessing an anti-inflammatory activity.

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Eupatilin inhibits lipopolysaccharide-induced expression of inflammatory mediators in macrophages

Cited by 66 publications

References 29 publications

In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Eupatilin induces human renal cancer cell apoptosis via ROS-mediated MAPK and PI3K/AKT signaling pathways

Artemisia asiaticaNakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways

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