In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Abstract

Artemisia species are important medicinal plants throughout the world. The present in vitro study, using a sesquiterpene lactone-bearing fraction prepared from Artemisia khorassanica (SLAK), sought to investigate immunomodulatory/anti-inflammatory properties of this plant and elucidate potential underlying mechanisms for the actions. Effects of the SLAK on mitogeninduced murine splenocyte proliferation and interleukin (IL)-4 and interferon (IFN)-secretion were evaluated. To assess anti-inflammatory activities, levels of inducible of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ), as well as expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in peritoneal macrophages was examined. The results showed that SLAK noticeably was capable of suppressing PHA/LPS-stimulated splenocyte proliferation and of up-regulating production of the T-helper (T H )-2 cell cytokine IL-4 while down-regulating formation of T H 1 IFN. In addition, while SLAK caused negligible proliferation inhibition, peritoneal macrophages displayed considerable decrease in NO and PGE 2 production along with iNOS and COX-2 expression. The current experiment shows Artemisia khorasanica -a traditionally used herb -may have immunomodulatory and anti-inflammatory effects. It is anticipated that the ingredients may be employed as therapeutic candidates in the regulation of some immune responses implicated in various conditions and ailments.

Anti-inflammatory effect of garlic 14-kDa protein on LPS-stimulated-J774A.1 macrophages

“…There is no doubt that the relationship between the immune system and human health is very intimate. Hence, many researchers have focused their efforts toward the examination of traditionally used natural products that possess immunomodulatory activity 16,17 .…”

“…Recently there has been great interest in possible antiinflammatory property of naturally derived compounds [16][17][18] . Aged garlic extract (AGE) was established to contain one major immunomodulatory protein present in R10 fraction called garlic 14-kDa protein which has been proved to impart a wide range of immunomodulatory properties [19][20][21][22][23] .…”

In vitro anti-inflammatory and immunomodulatory properties of umbelliprenin and methyl galbanate

“…Accordingly, the impact of medicinal plants/their derivatives on aspects of immune function has been a focus of many researchers seeking to discover efficient compounds with antiinflammatory and immunomodulatory potentials (Lopez-Varela et al, 2002;Zamani Taghizadeh Rabe et al, 2015). Even though some studies have already focused on some aspects of the immunomodulatory/anti-inflammatory effects of Ferula spp.…”

Plant-based Immunomodulators and Their Potential Therapeutic Actions

“…Moreover, treatment with sesquiterpene lactones shifts an IFN-γ-based Th1 response to an IL-4-producing Th2 response, highlighting their therapeutic potential. 171 Artemisinin, thapsigargin, and parthenolide are the sesquiterpene lactones that have been approved for clinical trials as anticancer and anti-inflammatory drugs. 172…”