2008
DOI: 10.1007/s10886-008-9539-7
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Evaluating Ascorbate Oxidase as a Plant Defense Against Leaf-Chewing Insects Using Transgenic Poplar

Abstract: Ascorbate is the major water-soluble antioxidant in plants and animals, and it is an essential nutrient for most insect herbivores. Therefore, ascorbate oxidase (AO) has been proposed to function as a plant defense that decreases the availability of ascorbate to insects. This hypothesis was tested by producing transgenic poplar (Populus tremula× Populus alba; Salicaceae) with 14-to 37-fold higher foliar AO activities than control (wild type) leaves and feeding these leaves to Lymantria dispar L. (Lepidoptera: … Show more

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Cited by 21 publications
(10 citation statements)
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“…Moreover, there was considerable variation in the AsA levels of the three transgenic lines tested, and only one of these lines had significantly lower AsA levels than untransformed controls. On this transgenic line, first-instar gypsy moth larvae had lower relative growth rates and fourthinstar larvae had lower efficiency of conversion of ingested matter to body mass compared to individuals reared on untransformed controls [67]. Thus, the possibility remains that decreasing AsA content in poplar foliage may decrease its suitability as a food source for gypsy moth larvae.…”
Section: Ascorbate As a Substrate For Plant Oxidasesmentioning
confidence: 97%
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“…Moreover, there was considerable variation in the AsA levels of the three transgenic lines tested, and only one of these lines had significantly lower AsA levels than untransformed controls. On this transgenic line, first-instar gypsy moth larvae had lower relative growth rates and fourthinstar larvae had lower efficiency of conversion of ingested matter to body mass compared to individuals reared on untransformed controls [67]. Thus, the possibility remains that decreasing AsA content in poplar foliage may decrease its suitability as a food source for gypsy moth larvae.…”
Section: Ascorbate As a Substrate For Plant Oxidasesmentioning
confidence: 97%
“…Moreover, when corn earworm larvae were fed a protein extract from soybean leaves that had confirmed AO activity, the larvae had significantly lower weight gain if their diet was also amended with 0.1% AsA [55]. The accumulation of DHA and H 2 O 2 in the insect gut may have been due to their generation within the plant tissues prior to ingestion, because subsequent studies have suggested that oxygen levels within the insect midgut are too low for AO to retain activity there [67,68]. To test the in vivo role of AO in limiting AsA availability and inducing resistance against herbivores, Barbehenn et al [67] over-expressed an AO gene from cucumber in transgenic hybrid poplar (P. tremula X P. alba).…”
Section: Ascorbate As a Substrate For Plant Oxidasesmentioning
confidence: 99%
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“…Ascorbate oxidase (AO) is a glycoprotein and a member of blue copper oxidase family that mediates ascorbate oxidation to dehydroascorbate (DHA) which usually follows high affinity transport across the membrane (Smirnoff, 2000; Szarka et al, 2004; Barbehenn et al, 2008; De Tullio et al, 2013). Dehydroascorbate (oxidized form) in the apoplast is then transported into cell cytosol and is exchanged with reduced form to ensure constant apoplast redox flux (Horemans et al, 2000; De Tullio et al, 2013; Anjum et al, 2014).…”
Section: Apoplast Ascorbate Levels and Expression Of Ascorbate Oxidasementioning
confidence: 99%
“…AsA participated in the dynamic antioxidant response of Norway spruce phloem attacked by Ips typographus L. [190]. Thus, decreasing the availability of AsA by AO activity might be a defense strategy in poplar against leaf-chewing insects [191]. In contrast, the AsA content increased in cabbage leaves after flea beetle attack [192], indicating differences between the insect attack responses in woody and nonwoody plants.…”
Section: The Involvement Of Asc In the Response To Biotic Stressmentioning
confidence: 99%