Evaluating in vitro and in vivo the interference of ascorbate and acetaminophen on glucose detection by a needle-type glucose sensor

Moatti-Sirat, Dinah; Velho, Gilberto; Réach, G.

doi:10.1016/0956-5663(92)85030-e

Cited by 64 publications

(37 citation statements)

References 9 publications

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“…Following early attempts to use permselective membranes between enzyme layer and electrode surface [1] or electropolymerized poly(phenylenediamine) films [2,3] for controlling the access of AA to the electrode a major breakthrough was seen in the application of free-diffusing redox mediator with significantly lower oxidation potential [4,5]. Other methods include the elimination of ascorbate from the matrix before detection, e.g., by means of a microfabricated flow-through reactor filled with ascorbate oxidase in flow-injection measurements [6], building multilayer sensors containing a layer of ascorbate oxidase [7] or incorporating interferencerejecting membranes [8,9]. More sophisticated techniques perform a simultaneous measurement of glucose and ascorbate, e.g., using differential measurements with two electrodes, one modified with glucose oxidase and the other lacking the enzyme [10] or containing inactivated enzyme [11] or the second electrode containing coimmobilized ascorbate oxidase [12].…”

Section: Introductionmentioning

confidence: 99%

SECM Visualization of Spatial Variability of Enzyme‐Polymer Spots. Part 2: Complex Interference Elimination by Means of Selection of Highest Sensitivity Sensor Substructures and Artificial Neural Networks

2006

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Polymer spots with entrapped glucose oxidase were fabricated on glass surfaces and the localized enzymatic response was subsequently visualized using scanning electrochemical microscopy (SECM) in the generator -collector mode. SECM images were obtained under simultaneous variation of the concentration of glucose (0 -6 mM) and ascorbic acid (0 -200 mM), or, in a second set of experiments, of glucose (0 -2 mM) and 2-deoxy-d(þ)-glucose (0 -4 mM). Aiming at the quantification of the mixture components discretization of the response surfaces of the overall enzyme/ polymer spot into numerous spatially defined microsensor substructures was performed. Sensitivity of sensor substructures to measured analytes was calculated and patterns of variability in the data were analyzed before and after elimination of interferences using principal component analysis. Using artificial neural networks which were fed with the data provided by the sensor substructures showing highest sensitivity for glucose, glucose concentration could be calculated in solutions containing unknown amounts of ascorbic acid with a good accuracy (RMSE 0.17 mM). Using, as an input data set, measurements provided by sensing substructures showing highest sensitivity for ascorbic acid in combination with the response of the sensors showing highest dependence on the glucose concentration, the error of the ascorbic acid concentration calculation in solution containing the unknown amount of glucose was 10 mM. Similarly, prediction of the glucose concentration in the presence of 2-deoxy-d(þ)-glucose was possible with a RMSE of 0.1 mM while the error of the calculation of 2-deoxy-d(þ)-glucose concentrations in the presence of unknown concentrations of glucose was 0.36 mM.

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Section: Introductionmentioning

confidence: 99%

SECM Visualization of Spatial Variability of Enzyme‐Polymer Spots. Part 2: Complex Interference Elimination by Means of Selection of Highest Sensitivity Sensor Substructures and Artificial Neural Networks

2006

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“…Many methods have been adopted for the detection of hydrogen peroxide, and using conventional electrode has been a feasible way to carry out the direct electrochemical detection of H 2 O 2 . However, it induced many disturbing substances for its high overpotential [1]. The direct detection, by catalyzing reduction of H 2 O 2 , could be taken through immobilizing peroxidases on the electrode.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behavior of hydrogen peroxide sensor based on new methylene blue as mediator

Zhu

2007

Front. Chem. China

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A novel amperometric hydrogen peroxide sensor was proposed by co-immobilizing new methylene blue (NMB) and Horseradish peroxidase (HRP) on glassy carbon electrode through covalent binding. The electrochemical behavior of the sensor was studied extensively in 0.1 mol/L phosphate buffering solution (pH = 7.0). The experiments showed NMB could effectively transfer electrons between hydrogen peroxide and glassy carbon electrode. The electron transfer coefficient and apparent reaction rate constant were determined to be 0.861 and 1.27 s −1 . The kinetic characteristics and responses of sensor on H 2 O 2 were investigated. The Michaelis constant is 8.27 mol/L and the linear dependence of current on H 2 O 2 is in the range of 2.5−100 µmol/L. At the same time, the effects of solution pH, buffer capacity, and temperature on the sensor were examined.

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“…The membrane coating enhances the signal-to-noise ratio of the response either by electrostatic repulsion [16,17] or by size exclusion [18,19]. In the first case a negatively charged membrane such as cellulose acetate or Nafion effectively separates the uncharged H 2 O 2 from the negatively charged interferents.…”

Section: Introductionmentioning

confidence: 99%

Interferent Suppression Using a Novel Polypyrrole-Containing Hydrogel in Amperometric Enzyme Biosensors

2002

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Evaluating in vitro and in vivo the interference of ascorbate and acetaminophen on glucose detection by a needle-type glucose sensor

Cited by 64 publications

References 9 publications

SECM Visualization of Spatial Variability of Enzyme‐Polymer Spots. Part 2: Complex Interference Elimination by Means of Selection of Highest Sensitivity Sensor Substructures and Artificial Neural Networks

SECM Visualization of Spatial Variability of Enzyme‐Polymer Spots. Part 2: Complex Interference Elimination by Means of Selection of Highest Sensitivity Sensor Substructures and Artificial Neural Networks

Electrochemical behavior of hydrogen peroxide sensor based on new methylene blue as mediator

Interferent Suppression Using a Novel Polypyrrole-Containing Hydrogel in Amperometric Enzyme Biosensors

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