Evaluating the DNA barcodes for identification of butterflies from Western Himalayas, Uttarakhand, India

Singh, Vinaya Kumar; Joshi, P. C.; Gupta, Sandeep

doi:10.1016/j.aspen.2021.03.008

Cited by 3 publications

(2 citation statements)

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“…Our sample size was low, and additionally with no representative conspeci c barcodes available in the database for comparison, the actual reason for this low interspeci c divergence remains debatable. Similar results have also been observed in other studies where the 3% threshold value, as well as the 10X rule, undervalued the actual species richness [22][23]. It is already established how an optimal threshold value will always be taxon-speci c and a universal threshold is likely to be ineffective even within a small group [47] and only a much more ne-grained robust genetic survey for these groups can provide a better understanding of the low interspeci c divergences, which can only be achieved with more barcode addition to the database.…”

Section: Discussionsupporting

confidence: 90%

“…Coming to the Indian scenario, the integration of molecular tools and traditional morphology for butter y taxonomy has seen little investigation, with only 601 available barcodes in BOLD representing 166 species. Signi cant contributions have been made by Gaikwad et al [22] and Singh et al [23] towards a curated reference library for the butter y fauna of Western Ghats and Western Himalaya respectively.…”

Section: Introductionmentioning

confidence: 99%

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Unique among high passes: Phylogenetic inferences from DNA barcoding of the butterfly fauna of Ladakh Trans-Himalaya, India

Ali,

Dey,

Das

et al. 2024

Preprint

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The butterfly assemblage of Ladakh Trans-Himalaya demands a thorough analysis of their population genetic structure owing to their typical biogeographic affinity and their adaptability to extreme cold-desert climates. No such effort has been taken till date, and in this backdrop, we created a barcode reference library of 60 specimens representing 23 species. Barcodes were generated from freshly collected leg samples using the Sanger sequencing method, followed by phylogenetic clade analyses and divergence calculation. Our data represents 22% of Ladakh’s Rhopaloceran fauna with the novel barcode submission for six species, including one Schedule II species, Paralasa mani. Contrary to the 3% threshold rule, the interspecific divergence between two species pairs of typical mountain genus Hyponephele and Karanasa was found to be 2.3% and 2.2%, respectively. The addition of conspecific global barcodes revealed that most species showed little increase in divergence value, while a two-fold increase was noted in a few species. Bayesian clade clustering outcomes largely aligned with current morphological classifications, forming monophyletic clades of conspecific barcodes, with only minor exceptions observed for the taxonomically complicated genus Polyommatus and misidentified records of Aulocera in the database. We also observed variations within the same phylogenetic clades forming nested lineages, which may be attributed to the taxonomic intricacies present at the subspecies level globally, mostly among Eurasian species. Overall, our effort not only substantiated the effectiveness of DNA Barcoding for the identification and conservation of this climatically vulnerable assemblage but also highlighted the significance of deciphering the unique genetic composition among this geographically isolated population of Ladakh butterflies.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%