Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi,<i>Salmo salar</i>

Abdel-Hakeem, Sara S.; Mahmoud, Ghada Abd‐Elmonsef; Abd‐Elhafeez, Hanan H.

doi:10.1017/s143192761901506x

Cited by 28 publications

(15 citation statements)

References 59 publications

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“…Negative images were performed using CMEIAS Color Segmentation, an improved computing technology used to process color images by segmenting the foreground object of interest from the background 64 . This has been done by the following steps: open the image with CMEIAS Color Segmentation, then select "Process" from the menu items, and subsequently choose "Negative image" [68][69][70][71] .…”

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confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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the current study investigated telocytes (tcs) in the intestinal bulb of Grass carp using light microscopy (LM), transmission electron microscopy (teM), scanning electron microscopy, and immunohistochemistry (iHc). By LM, tcs were distinguished by the typical morphological features that had a cell body and telopodes using He, toluidine blue, methylene blue, Marsland silver stain, Grimelius's silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon's and Mallory triple trichrome, Van Gieson stains, Verhoeff's stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial tcs were closely related to the endocrine cells. tcs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity. Interstitial cells have critical roles in the maintenance of the appropriate 3D scaffold and functional requirements of the organs. Telocytes (TCs) are the cell population that forms a network through a labyrinthine system formed by telopodes. They are long and slender prolongations reached up to hundreds of microns. Telopodes are identified by their segments; the thin segment, podomer and the thick segment, the podoms. TCs established contact to other resident cells or to wandering cells via their telopodes or via the cell body. Telopodes network provide-long-distance cell-cell signaling (intercellular communication) 1. Paracrine signaling is critical for TCS function. TCs produce several secretory molecules and factors contributing to the functional significance.Gene analysis 2 and proteomics analysis 3 are investigated for TCs. The functional contributions of TCS are supposed to be related to angiogenesis 4 , and development, maintenance of homeostatic balance 4 , immunosurveillance 5 , tissue regeneration and repair through providing adequate microenvironment for stem cell niche and promoting their differentiation 6,7. TCs are identified in a wide variety of organs from diverse species including mammals 8-11 , avian 12,13 , reptiles 14 , Amphibians 15 and aquatic species 16,17 and parasitic worm 18. They are located in the trachea and lungs 19 , heart 20 and the blood vessels 21 , kidney, ureter, urinary bladder 22 , tongue 23 , oesophagus, stomach and small and large

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confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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“…The paraffin-embedded sections were stained according to the procedure of Hoff et al (1985) with modifications according to Abdel-Hakeem et al (2019); Abdel-Maksoud et al (2019); Soliman (2019 a ); Soliman et al (2019); Yousef et al (2019); and Mahmoud et al (2020). The sections were then analyzed using a Leitz DM 2500 microscope with an external fluorescent unit (Leica EL 6000).…”

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confidence: 99%

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

et al. 2020

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Studying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

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“…R. toruloides MH023518 was isolated from Egyptian faba bean roots as carotene target yeast using dilution plate method on yeast extract peptone dextrose (YPD) medium (dextrose 20, yeast extract 10, peptone 20 and agar 20 g L −1 ) and stored at 4°C. [ 37,38 ] The red yeast was identified first according to its phenotypic characteristics including colony characteristics, color, ascospore formation, pseudohyphae, and the physiological characteristics, [ 39 ] and the identification was confirmed by genotypic characters. For molecular identification, yeast culture was prepared in 250 ml Erlenmeyer baffle flask containing 50 ml of the YPD medium incubated at 25 ± 1°C for 2 days.…”

Section: Methodsmentioning

confidence: 99%

Chemical‐ vs sonochemical‐assisted synthesis of ZnO nanoparticles from a new zinc complex for improvement of carotene biosynthesis fromRhodotorula toruloidesMH023518

Ibrahim

Mahmoud

2020

Applied Organom Chemis

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The complex [ZnL2] {HL = 2‐(((3,4‐dimethylphenyl)imino)methyl)phenol} was introduced by two routes (chemical vs. sonochemical) and acted as a source of different size zinc‐oxide (ZnO) nanoparticles (NPs) (nonsonicated ZnO NPs of Brunauer–Emmett–Teller (BET) surface area of 19.208 m2 g−1, total pore volume of 0.03493 cc g−1, and particle diameter of 20.49–114.89 nm vs. sonicated ZnO NPs of BET surface area of 38.383 m2 g−1, total pore volume of 0.08723 cc g−1 and particle diameter of 15.09–55.31 nm). The NPs generate cell stress that leads to the formation of reactive oxygen species (ROS). Consequently, carotene biosynthesis can be enhanced to overcome the ROS. Rhodotorula toruloides MH023518, genetically identified with 18S rRNA, produced carotenoids (124 ± 4.5 mg L−1) and lipids (0.897 ± 0.01 mg L−1) under no NPs' stress, but these values increased to total carotenoids of 264 ± 0.6 and 297.78 ± 0.8 mg L−1 and total lipids of 0.97 ± 0.007 and 1.096 ± 0.015 mg L−1 under stress conditions of 50 ppm of the nonsonicated and sonicated ZnO NPs, respectively. These results may represent potential industrial utilization of the as‐formed ZnO NPs (particularly the sonicated sample) as carotene and lipid stimulator. Additionally in this article, the soluble proteins, total antioxidants, and catalase and superoxide dismutase specific activities of the yeast in the presence of various concentrations of the as‐formed ZnO NPs were determined.

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Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi,Salmo salar

Cited by 28 publications

References 59 publications

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

Chemical‐ vs sonochemical‐assisted synthesis of ZnO nanoparticles from a new zinc complex for improvement of carotene biosynthesis fromRhodotorula toruloidesMH023518

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