Evaluation for anti-inflammatory effects of<i>Siegesbeckia glabrescens</i>extract<i>in vitro</i>

Lee, J.H.; Kang, Bong-Seok; Hwang, Keum Hee; Kim, G.H.

doi:10.1080/09540105.2010.549210

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…Our results corroborate with the previous report on the ability of Se supplementation to reduce iNOS expression by 3 fold as compared to SeDef LPS unstimulated RAW 264.7 macrophages (Prabhu et al, 2002). Similar results were seen with the role of Aster Yomena (Kim et al, 2017), Eupartoium makinoi (Ahn et al, 2015), Siegesbeckia glabrescens (Lee, Kang, Hwang, & Kim, 2011) and lansai Cand D (Taechowisan, Wanbanjob, Tuntiwachwuttikul, & Liuin, 2010) in down-regulation of iNOS transcription along with NO quenching which further decreases COX-2 and PGE 2 mRNA levels in LPS induced macrophages.…”

Section: Resultssupporting

confidence: 81%

Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages

Dhanjal

Sharma

Prabhu

et al. 2017

Food and Agricultural Immunology

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The accessibility of selenium from naturally enriched sources such as cereals crops can potentially be used as selenium supplements to support nutritional requirements. Dietary selenium supplementation, as Se-rich wheat extracts, on RAW264.7 macrophage cells enhanced the antioxidant capacity via augmentation of cellular selenoprotein glutathione peroxidase 1 (GPx-1) expression in the absence or presence of lipopolysaccharide (LPS) treatment. Cells were supplemented with Se in the form of sodium selenite (SS), seleniferous wheat extract (SeW) and seleniferous wheat extract with rMETase treatment (SeW+rMET) at three different concentrations. Cells supplemented with SS and SeW+rMET showed increase in GPx-1 expression as compared to SeW treated cells. SeW+rMET, further, down-regulated the LPS-induced expression of cyclooxygenase-2, microsomal PGE synthase-1 and inducible nitric oxide synthase w.r.t. Se-deficient cells, while the expression of hematopoietic PGD synthase was upregulated. This demonstrates SeSup effectively modulates the expression inflammatory responses, indicating the potential benefits of dietary selenium supplementation.

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Section: Resultssupporting

confidence: 81%

Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages

Dhanjal

Sharma

Prabhu

et al. 2017

Food and Agricultural Immunology

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“…iNOS and cOX-2 are widely known as the main regulatory factors in inflammatory responses; thus, iNOS and COX-2 expression levels have been examined by many studies for the development of anti-inflammatory agents (21,25,34). As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Kaempferol‑3‑O‑β‑rutinoside suppresses the inflammatory responses in lipopolysaccharide‑stimulated RAW264.7 cells via the NF‑κB and MAPK pathways

Hwang

Kang

et al. 2019

Int J Mol Med

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Kaempferol-3-O-β-rutinoside is one of the compounds isolated from tartary buckwheat (Fagopyrum tatricum), and its biological effects have not been studied yet. The present study examined the anti-inflammatory effects of kaempferol-3-O-β-rutinoside and explore its regulatory mechanisms in lipopolysaccharide (LPS)-induced macrophage RAW264.7 cells. Kaempferol-3-O-β-rutinoside exhibited no cytotoxic effect in RAW 264.7 macrophage and 293 cell lines up to 300 µM. As the concentration of kaempferol-3-O-β-rutinoside was increased, the activity of nitric oxide was inhibited in LPS-stimulated RAW264.7 cells. In addition, kaempferol-3-O-β-rutinoside treatment downregulated the expression of inflammation-related cytokines tumor necrosis factor-α and interleukin-6 in LPS-stimulated RAW264.7 cells. Furthermore, kaempferol-3-O-β-rutinoside treatment suppressed inflammatory-mediated factors, such as inducible nitric oxide synthase and cyclooxyganse-2. These inflammation-related proteins are known to be regulated by NF-κB and mitogen-activated protein kinase (MAPK) signaling, therefore the effect of kaempferol-3-O-β-rutinoside on these pathways was investigated. The results demonstrated that kaempferol-3-O-β-rutinoside decreased the expression of inhibitor of κB (IκB) protein and IκB kinases; as a result, the nuclear translocation and expression of NF-κB was inhibited in LPS-stimulated RAW264.7 cells. Furthermore, kaempferol-3-O-β-rutinoside inhibited the phosphorylation of p38, extracellular signal-regulated kinase and stress-activated protein kinase in LPS-stimulated RAW264.7 cells. Thus, the present data demonstrated that kaempferol-3-O-β-rutinoside suppressed inflammation-related gene expression through the NF-κB and MAPK pathways, and suggested that it may be a useful reagent in pharmacological research.

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“…MTT was converted by intact mitochondrial reductase and precipitated as blue crystals during a four hour contact period. The supernatants were gently removed and the formazan crystals were resolved in 100 ml acidic isopropanol (0.04 M HCl in isopropanol), and absorbance was read at 540 nm with a plate reader (Stat-Fax 2100, USA) (Abuharfeil, Al-Oran, & Abo-Shehada, 1999;Lee, Kang, Hwang, & Kim, 2011).…”

Section: Viability Assaymentioning

confidence: 99%

Cytotoxic effects ofCuscutaextract on human cancer cell lines

Ghazanfari

Naseri

Shams

et al. 2013

Food and Agricultural Immunology

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Cancer is a growing health problem around the world. Although there are different therapeutic methods for cancer such as lymphoma and melanoma cancers, none of them have possessed complete efficacy up to now. Therefore, discovery of novel anti-cancer drugs is important. In this study, the cytotoxic effect of Cuscuta extract, a traditional Iranian medicinal herb, on melanoma cell line (SK-MEL-3) and human Burkitt lymphoma (Raji) is evaluated. MTT assay was performed for cytotoxic effect of Cuscuta extract. The most cytotoxic effects of Cuscuta extract on SK-MEL-3 and Raji cell lines were 80 and 81%, respectively, compared to a control group. According to our data, Raji cells are more sensitive to Cuscuta than the SK-MEL-3 cells. Cuscuta extract seems to be a good candidate as an anti-cancer agent against lymphoma and melanoma cancers. To clarify the effective molecules and their mechanisms, further studies are undertaken in our laboratory on animal models and humans.

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Evaluation for anti-inflammatory effects ofSiegesbeckia glabrescensextractin vitro

Cited by 8 publications

References 21 publications

Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages

Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages

Kaempferol‑3‑O‑β‑rutinoside suppresses the inflammatory responses in lipopolysaccharide‑stimulated RAW264.7 cells via the NF‑κB and MAPK pathways

Cytotoxic effects ofCuscutaextract on human cancer cell lines

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