An experiment was conducted to compare rice straw mat and kanchi (bamboo sticks) as substrates in periphyton-based polyculture systems. The experiment had three treatments: (a) no substrate (control), (b) rice straw as a substrate (3 Â 2.7 kg pond À1 ) and (c) kanchi as a substrate (390 kanchi pond À1 ). Fingerlings (n 5 40) of rohu, Labeo rohita (24.5 AE 0.5 g); mrigal, Cirrhinus mrigala (25.1 AE 0.6 g); catla, Catla catla (25.8 AE 0.5 g); common carp, Cyprinus carpio (27.6 AE 0.6 g), and silver carp, Hypophthalmichthys molitrix (30.4 AE 0.9 g) were stocked at a 3:2:2:2:1 ratio and cultured for 90 days. There were no di¡erences in the number of plankton, periphyton and macro-zoobenthos among the treatments. The total plate count of bacteria was higher in the rice straw treatment (41320 millioncfu m À 2 ) than that in the kanchi treatment (11780 million cfu m À 2 ). Growth and the ¢nal mean weight of rohu, catla and common carp were higher in the substrate treatments than those in the control. Rice straw and kanchi treatment, respectively, resulted in 38% and 47% higher combined total weight gain over control. Gross margin analysis showed that rice straw treatment resulted in more pro¢t than the control and kanchi treatment. Therefore, rice straw has the potential to be used to increase production in the low-input rural aquaculture.