Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection

Kumarasamy, Verasingam; Wahab, Ahmad; Chua, S.K.; Hassan, Zuhair Muhammad; Chem, Y K; Mohamad, Maria Safura; Chua, Kaw Bing

doi:10.1016/j.jviromet.2006.11.001

Cited by 196 publications

(177 citation statements)

References 19 publications

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“…This assay provides additional dengue diagnostic tool to the existing approaches of PCR, antibody capture ELISA and virus isolation 5,6,10,12,13,20,24,25,28 . Even though NS1 is a sensitive methodology for the detection of DENV it should never be used as the only methodology for DENV detection because it doesn't identify different serotypes 39 .…”

Section: Discussionmentioning

confidence: 99%

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Colombo

Vedovello

Reis

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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Section: Discussionmentioning

confidence: 99%

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Colombo

Vedovello

Reis

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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“…Evaluation of these assays (Dussart et al 2006, Kumarasamy et al 2007, Blacksell et al 2008, Lapphra et al 2008) has demonstrated high sensitivity and specificity for DENV detection, particularly during the acute phase of the disease. In this study, we evaluated the performance of three commercially available dengue NS1 antigen assays, in comparison with other widely used dengue diagnostic assays, to determine the usefulness of NS1 antigen assays in early diagnosis of dengue in a primary healthcare setting in Singapore.…”

Section: Vector-borne and Zoonotic Diseasesmentioning

confidence: 99%

Evaluation of Nonstructural 1 Antigen Assays for the Diagnosis and Surveillance of Dengue in Singapore

Pok

Lai

Sng

et al. 2010

Vector-Borne and Zoonotic Diseases

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Early and accurate diagnosis of dengue is imperative for disease surveillance, which helps in the control of dengue in endemic countries. In this study, we evaluated the performance of three commercially available dengue nonstructural 1 (NS1) antigen assays (Bio-Rad PlateliaÔ Dengue NS1 Antigen ELISA, PanBio Dengue Early ELISA, and Bio-Rad Dengue NS1 Antigen Strip test) and compared them with reverse-transcription polymerase chain reaction (RT-PCR) and other commercially available serological assays for the diagnosis of dengue. The analysis showed RT-PCR to be the most sensitive and specific (100%) diagnostic method during the first 3 days of fever. The overall sensitivity of dengue NS1 antigen assays within the same period was 81.7%, indicating their potential role as a cost-effective and convenient alternative method to RT-PCR for the diagnosis of dengue fever in a primary healthcare setting. However, reduced sensitivity in detecting secondary dengue infections was one of the drawbacks of dengue NS1 antigen assays. Nonetheless, it remains a useful assay for the early detection of dengue and hence could play an important role in routine surveillance efforts to control dengue outbreaks in Singapore.

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“…Recently, commercially available kits have been developed for the rapid detection of dengue infections. [4][5][6][7][8] These kits are designed based on the principle of detecting the presence of NS1 antigen and/or anti-dengue antibodies in the blood of suspected dengue patients.…”

Section: Introductionmentioning

confidence: 99%

Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IGM, and IGG

Wang

Sekaran

2010

The American Society of Tropical Medicine and Hygiene

152

129

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A commercial Dengue Duo rapid test kit was evaluated for early dengue diagnosis by detection of dengue virus NS1 antigen and immunoglobulin M (IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-house IgM capture enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition assay, and the SD Dengue Duo rapid test. Of the 320 dengue acute and convalescent sera, dengue infection was detected by either serology or RT-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies. The sensitivity and specificity in diagnosing acute dengue infection in the SD Duo NS1/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific. Detection of both NS1 and IgM by SD Duo gave comparable detection rate by either serology or RT-PCR.

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Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection

Cited by 196 publications

References 19 publications

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Evaluation of Nonstructural 1 Antigen Assays for the Diagnosis and Surveillance of Dengue in Singapore

Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IGM, and IGG

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