Evaluation of a membrane desolvator for LC-ICP-MS analysis of selenium and platinum species for application to peptides and proteins

Abstract

MD gradient elution LC-ICP-MS analysis with 100% methanol was achieved. Species-independent sensitivity was maintained for Pt but lost for Se.

Understanding the relationship between viral infections and trace elements from a metallomics perspective: implications for COVID-19

“…To minimize the presence of interferent compounds that can compromise the detection sensitivity of the analytical method, the sample treatment procedure is generally required. 19 , 22 , 103 Several types of sample preparation are described in the literature to remove interferents, such as solid-phase extraction and its miniaturization strategies, 107–112 liquid–liquid extraction, and its miniaturization approaches, 113–116 cloud point extraction, 117 ionic liquid extraction, 118–121 membrane-associated solvent extraction 24 , 114 and ultrasound-assisted extraction. 22 , 99 , 122 Many of these methods are examples of sample preparation approaches used in studies focusing on metallobiomolecules.…”

“…17–19 In metallomics, metal determination requires extremely sensitive analytical tools, such as inductively coupled plasma optical emission spectroscopy (ICP OES 20 ) and inductively coupled plasma mass spectrometry (ICP-MS 21 , 22 ). Furthermore, as many metals are linked to biomolecules (proteins and metabolites), separation techniques, such as liquid chromatography (LC) 23 , 24 and capillary electrophoresis (CE), 25 , 26 have been required in metallomics studies. LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry), 27 , 28 SIMS (secondary ion mass spectrometry), 29 , 30 X-ray fluorescence spectrometry (XRF), 31 , 32 and LIBS (laser-induced breakdown spectroscopy) 33 , 34 are also important techniques to obtain information on the spatial distribution of metals in solid samples, such as tissues and cells.…”

Recent advances in flow injection analysis

“…This can be successfully eliminated by the use of an on-line membrane desolvator reported recently for LC-ICP-MS analysis of Se and Pt species for application for peptides and proteins. 353 It was shown in flow-injection measurements with the use of developed desolver that several organoselenium species can be measured in 50% methanol, and inorganic Pt and organoplatinum species in 50% acetonitrile. A very advanced ICP-MS procedure was developed for flow-injection sandwich assay of protein ferritin combined with antibody labeling with ruthenium chelate.…”

Investigation of Elemental Mass Spectrometry in Pharmacology for Peptide Quantitation at Femtomolar Levels

“…We report in this paper the quantitation of a selenium-containing peptide bearing two Sec residues in the sequence by LC-ICP-MS. Although specific pieces of equipment relying either on capillary liquid chromatography [ 11 ] or desolvator system [ 12 ] have been described to optimize sensitivity, we aimed at using conventional macrobore liquid chromatography hyphenated with an ICP-MS instrument routinely used for analyses of organic matters [ 13 ]. Such instrumental configuration includes a cooled spray chamber and oxygen in the nebulizer gas.…”