Evaluation of a Modified Cefsulodin-Irgasan-Novobiocin Agar for Isolation of Yersinia spp

Tan, Lai Kuan; Ooi, Peck Toung; Carniel, Élisabeth; Thong, Kwai Lin

doi:10.1371/journal.pone.0106329

Cited by 19 publications

(13 citation statements)

References 26 publications

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“…Furthermore, pathogenic strains sometimes grow as very small colonies missing the typical red centre on the CIN plates (18,42). Chromogenic-based agar plates have become popular in recent years for isolation and identification of Y. enterocolitica belonging to pathogenic biotypes (42).…”

Section: Identification Of Yersinia Sppmentioning

confidence: 99%

Identification of Yersinia at the Species and Subspecies Levels Is Challenging

Fredriksson-Ahomaa

Joutsen

Laukkanen‐Ninios

2018

Curr Clin Micro Rpt

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The genus Yersinia currently includes species, of which Y. enterocolitica and Y. pseudotuberculosis are enteropathogenic. The identification of Y. enterocolitica in particular is very demanding, because it consists of a group of very heterogeneous bacteria, including pathogenic and non-pathogenic strains. The aim of the review is to provide recent information on the characteristics and identification of Yersinia spp. and sources of enteropathogenic Yersinia spp. Identification of Yersinia spp. is still mainly based on biochemical tests and serotyping, but molecular methods have increasingly also been used. Sequencing the whole genome enables more accurate identification of enteropathogenic Yersinia spp. Pathogenic Y. enterocolitica strains of different bioserotypes have newly been identified from various animal sources. Moreover, the virulence gene ail has been detected in non-pathogenic Yersinia strains, especially from wild animals. Correct identification of pathogenic Yersinia strains is essential in assessing the health risk for humans and animals.

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Section: Identification Of Yersinia Sppmentioning

confidence: 99%

Identification of Yersinia at the Species and Subspecies Levels Is Challenging

Fredriksson-Ahomaa

Joutsen

Laukkanen‐Ninios

2018

Curr Clin Micro Rpt

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“…Despite numerous reports on the occurrence of Y. enterocolitica among humans, pigs or wild boars, the detection and identification are still difficult due to an overgrowth by bacteria from poly-contaminated samples, unusual growth characteristics of Y. enterocolitica and arduous discrimination of its colonies from other bacteria exhibiting Yersinia -like colonies on cefsulodin-irgasan-novobiocin Agar (CIN) such as Citrobacter freundii , C. braakii , Aeromonas hydrophila, Enterobacter cloacae , Providencia rettgeri , and Morganella morganii [ 10 – 12 ]. The recommended isolation of Y. enterocolitica is carried out by using ISO 10273–2003 method [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…To isolate presumptive strains of Y. enterocolitica, the ITC broth and CIN agar media are recommended [ 3 , 4 ]. CIN agar was devised by Schiemann in 1979 and is still useful in Y. enterocolitica isolation [ 10 – 12 , 19 – 21 ]. Morphology of Yersinia sp.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Yersinia enterocolitica isolates from humans, pigs and wild boars by MALDI TOF MS

et al. 2018

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BackgroundYersinia enterocolitica is widespread within the humans, pigs and wild boars. The low isolation rate of Y. enterocolitica from food or environmental and clinical samples may be caused by limited sensitivity of culture methods. The main goal of present study was identification of presumptive Y. enterocolitica isolates using MALDI TOF MS. The identification of isolates may be difficult due to variability of bacterial strains in terms of biochemical characteristics. This work emphasizes the necessity of use of multiple methods for zoonotic Y. enterocolitica identification.ResultsIdentification of Y. enterocolitica isolates was based on MALDI TOF MS, and verified by VITEK® 2 Compact and PCR. There were no discrepancies in identification of all human’ and pig’ isolates using MALDI TOF MS and VITEK® 2 Compact. However three isolates from wild boars were not decisively confirmed as Y. enterocolitica. MALDI TOF MS has identified the wild boar’ isolates designated as 3dz, 4dz, 8dz as Y. enterocolitica with a high score of matching with the reference spectra of MALDI Biotyper. In turn, VITEK® 2 Compact identified 3dz and 8dz as Y. kristensenii, and isolate 4dz as Y. enterocolitica. The PCR for Y. enterocolitica 16S rDNA for these three isolates was negative, but the 16S rDNA sequence analysis identified these isolates as Y. kristensenii (3dz, 4dz) and Y. pekkanenii (8dz). The wild boar’ isolates 3dz, 4dz and 8dz could not be classified using biotyping. The main bioserotype present within pigs and human faeces was 4/O:3. It has been shown that Y. enterocolitica 1B/O:8 can be isolated from human faeces using ITC/CIN culturing.ConclusionThe results of our study indicate wild boars as a reservoir of new and atypical strains of Yersinia, for which protein and biochemical profiles are not included in the MALDI Biotyper or VITEK® 2 Compact databases. Pigs in the south-west Poland are the reservoir for pathogenic Y. enterocolitica strains. Four biochemical features included in VITEK® 2 Compact known to be common with Wauters scheme were shown to produce incompatible results, thus VITEK® 2 Compact cannot be applied in biotyping of Y. enterocolitica.

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“…Y. pseudotuberculosis infection in humans was more likely to be severe than Y. enterocolitica infection 12) . However, it is also more difficult to isolate Y. pseudotuberculosis on CIN because of the presence of cefsulodin and inability to distinguish other mannitol-fermenting bacteria 6), 13) . In this study, all Y. pseudotuberculosis strains were able to grow on CIN with/without supplements but grew on CAYe without supplements.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Chromogenic Medium for Selective Isolation of <i>Yersinia</i>

Thuan

Naher

Kubo

et al. 2016

Journal of the Food Hygienics Society of Japan

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Cefsulodin-irgasan-novobiocin agar (CIN) has been used as a selective agar to detect Yersinia in food or human patients; however, its components can inhibit the growth of some strains of Yersinia isolates grew on both media after 48 hr of incubation at 32 . Of the 10 pathogenic Y. enterocolitica O3 which could not grow on CIN or CAYe, 9 strains could not grow on CIN with supplements and 1 strain could not grow CAYe with supplements. Of 9 strains which did not grow on CIN with supplements, 3 strains could not grow on CIN without supplements. However, 1 strain which did not grow on CAYe with supplements could grow on CAYe without supplements. All of the Y. pseudotuberculosis strains could grow on CIN with/without supplements and on CAYe without supplements. The results indicate that the inhibition of the growth of Y. enterocolitica O3 on CIN is related to the components of CIN; however, the inhibition on CAYe appears to be related to the supplements in CAYe. Therefore, CAYe may be a more useful selective medium than CIN for pathogenic Y. enterocolitica .

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Evaluation of a Modified Cefsulodin-Irgasan-Novobiocin Agar for Isolation of Yersinia spp

Cited by 19 publications

References 26 publications

Identification of Yersinia at the Species and Subspecies Levels Is Challenging

Identification of Yersinia at the Species and Subspecies Levels Is Challenging

Identification of Yersinia enterocolitica isolates from humans, pigs and wild boars by MALDI TOF MS

Evaluation of Chromogenic Medium for Selective Isolation of <i>Yersinia</i>

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