Evaluation of aminoalkylmethacrylate nanoparticles as colloidal drug carrier systems. Part II: characterization of antisense oligonucleotides loaded copolymer nanoparticles

Zobel, H.-P; Stieneker, F; Aziz, Shaheen; Gilbert, Matthias; Werner, D.; Noe, Christian R.; Kreuter, Jörg; Zimmer, Andreas

doi:10.1016/s0939-6411(99)00003-x

Cited by 46 publications

(25 citation statements)

References 19 publications

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“…Many studies reported nanoparticle hemolytic propeties10-25. The majority of these studies utilized a spectrophotometric detection of plasma-free hemoglobin, and none of them reported specific controls to rule out nanoparticle interference.…”

Section: Discussionmentioning

confidence: 99%

“…Determination of hemolytic properties is one of the most common tests in studies of nanoparticle interaction with blood components7-25. Interpreting the results of these studies is complicated due to variability in experimental approaches and a lack of universally accepted criteria for determination of the test-result validity.…”

Section: Introductionmentioning

confidence: 99%

“…Interpreting the results of these studies is complicated due to variability in experimental approaches and a lack of universally accepted criteria for determination of the test-result validity. Most in vitro studies of particle-induced hemolysis7-14, 17-20, 22-25 evaluate the percent hemolysis by spectrophotometriclly detecting plasma free hemoglobin derivatives after incubating the particles with blood and then separating undamaged cells by centrifugation. The incubation time, wavelength at which hemoglobin is quantified, and blood conditions (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Method for Analysis of Nanoparticle Hemolytic Properties in Vitro

et al. 2008

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Hemolysis (destruction of red blood cells) in vivo can lead to anemia, jaundice and other pathological conditions, therefore the hemolytic potential of all intravenously administered pharmaceuticals must be evaluated. Nanotechnology-derived devices and drug carriers are emerging as alternatives to conventional small-molecule drugs, and in vitro evaluation of their biocompatibility with blood components is a necessary part of early preclinical development. The small size and unique physicochemical properties of nanoparticles may cause their interactions with erythrocytes to differ from those observed for conventional pharmaceuticals, and may also cause interference with standardized in vitro tests. Separating true hemolytic responses from the false-positive or false-negative results caused by particle interference is important for correct interpretation of these tests. Here we describe validation of an in vitro assay for the analysis of nanoparticle hemolytic properties, and discuss observed nano-interferences with the assay. We propose alternative methods to avoid misleading results from nanoparticles, and discuss the potential relevance of nanoparticle in vitro hemolytic properties to in vivo systems.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Method for Analysis of Nanoparticle Hemolytic Properties in Vitro

et al. 2008

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“…Because of such commonalities, monkeys have been used to assess the toxic and therapeutic potential of inhaled substances (e.g., Monticello et al, 1989;Nikula et al, 1997;Petruska et al, 1997;Puri et al, 1987;Zobel et al, 1999) and have been the focus of research on nasal clearance . Macaque monkeys (typically rhesus or cynomolgus) are most commonly used in such research, although an increasing trend in the United Kingdom pharmaceutical industry is to use the common marmoset for preclinical toxicology programs (Smith et al, 2001).…”

mentioning

confidence: 99%

Inertial Particle Deposition in a Monkey Nasal Mold Compared with that in Human Nasal Replicas

Kelly

Asgharian

Wong

2005

Inhalation Toxicology

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Information on nasal particle deposition is used in risk assessments for exposure to airborne particulate pollutants and for optimizing the delivery of therapeutic aerosols. Monkeys are commonly used to assess the therapeutic potential of inhaled substances and to a lesser extent the toxicity of inhaled xenobiotics. Yet no reliable measurements of the deposition efficiency of monkey nasal airways for particles >1 microm have been reported to date. The goals of this study were to measure the deposition efficiency (>1 microm) of a replica of monkey nasal airways and to investigate potential differences in nasal deposition between humans and monkeys by comparing results with similar measurements recently reported for human nasal replicas. The monkey nasal replica was an acrylic mold made from a postmortem cast of the nasal airways of a 12-kg, male rhesus monkey. Particle deposition in the monkey nasal mold was measured for monodisperse aerosols between 1 and 10 microm and constant inspiratory flow rates between 2 and 7 lpm. Total deposition efficiency increased from nearly 0 to 100% with increasing particle inertia and was uniquely determined by values of an inertial impaction parameter. The deposition efficiencies of the monkey replica agreed well with those of human nasal replicas when compared according to equivalent Stokes numbers based on minimum cross-sectional area. Results from this study could improve monkey-to-human extrapolation models and interpretations of data from particle toxicity and therapeutic aerosol studies using monkeys.

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“…Several investigations have shown that nanoparticles can not only improve the stability of therapeutic agents against enzymatic degradation and control the release of therapeutic agents, but also can be conveyed to remote target sites either by localized delivery using a catheter-based approach with a minimal invasive procedure, or they can be conjugated to a biospecific ligand which could lead them to the target tissue or organ [7,8]. Also, it has been recognized that nanoparticles due to their small size and the extremely large surface area per unit volume have unique characteristics and so can be used as biosensors [5,[9][10][11][12]. Various nanostructures, generally providing a large surface area for the immobilization of enzyme molecules, have been actively developed for enzyme stabilization [1].…”

Section: Introductionmentioning

confidence: 99%

Stability and enzyme activity of lysozyme in the presence of Fe3O4 nanoparticles

et al. 2015

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The goal of this work was investigation the effect of adsorption the Fe 3 O 4 as magnetic nanoparticle on lysozyme activity and thermal stability. The turbidimetric assay (activity) of lysozyme was followed by the decreased optical density of a turbid cell suspension (about 0.3 mg/cm 3 Micrococcus lysodeikticus) photometrically at 450 nm. In this study, the effect of nano-Fe 3 O 4 on lysozyme activity was investigated by UV-Vis spectrophotometry at pH = 7.25 at 35°C using sodium phosphate buffer. Measurements were carried out using 6 9 10 -8 M (0.1 mg/cm 3 ) concentration of lysozyme and a range of nano-Fe 3 O 4 concentrations between 0 and 0.06 mg/cm 3 . It was found that by increasing nanoFe 3 O 4 , the rate of M. lysodeikticus lyses (lysozyme activity) increases. The thermal stability of lysozyme was investigated in the presence of nano-Fe 3 O 4 over the temperature range of 293-363 K in sodium phosphate buffer and pH = 7.25. Results indicated that by increasing nano-Fe 3 O 4 , the thermal stability of lysozyme increases. Graphical abstract

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Evaluation of aminoalkylmethacrylate nanoparticles as colloidal drug carrier systems. Part II: characterization of antisense oligonucleotides loaded copolymer nanoparticles

Cited by 46 publications

References 19 publications

Method for Analysis of Nanoparticle Hemolytic Properties in Vitro

Method for Analysis of Nanoparticle Hemolytic Properties in Vitro

Inertial Particle Deposition in a Monkey Nasal Mold Compared with that in Human Nasal Replicas

Stability and enzyme activity of lysozyme in the presence of Fe3O4 nanoparticles

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