Evaluation of anti-lipase activity and bioactive flavonoids in the Citri Reticulatae Pericarpium from different harvest time

Zeng, Su-Ling; Li, Shang-Zhen; Lai, Chang-Jiang-Sheng; Wei, Minyan; Chen, Baizhong; Li, Ping; Zheng, Guodong; Liu, E‐Hu

doi:10.1016/j.phymed.2018.04.008

Cited by 67 publications

(45 citation statements)

References 23 publications

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“…They also has a high edible value and a wide range of applications, being used in medicated diet and food therapy (X. Yu et al, ). Modern pharmacology studies indicate that CRP has a wide range of pharmacological antioxidant (Yi, Yu, Liang, & Zeng, ) and anti‐inflammatory effects (Chen, Tait, & Kitts, ), improves the cardiovascular system and regulates lipids in the body (J. Yu, Su, & Lv, ), has a protective effect on the liver (Green, Wheatley, McGrowder, Dilworth, & Asemota, ), suppresses adipogenesis (Sheu, Chuang, & Chien, ) and anti‐lipase activity (Zeng et al, ). The pathogenesis of hyperlipidemia is related to oxidative stress, inflammatory response, lipid metabolism, etc.…”

Section: Discussionmentioning

confidence: 99%

Nontargeted urine metabolomics analysis of the protective and therapeutic effects of Citri Reticulatae Chachiensis Pericarpium on high‐fat feed‐induced hyperlipidemia in rats

Zeng

Huang

Luo

et al. 2020

Biomedical Chromatography

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In this study, we focused on studying the changes in urine metabolites in hyperlipidemic rats using ultra‐performance liquid chromatography coupled with quadrupole time‐of‐fight mass spectrometry (UPLC–Q‐TOF/MS) and metabolomics, as well as the effect of Citri Reticulatae Chachiensis Pericarpium (CRCP) on hyperlipidemia. These urine samples were examined by UPLC–Q‐TOF/MS to obtain MS data. The MS data were analyzed by principal component analysis and partial least squares‐discriminant analysis to identify the differential metabolites. CRCP reduced the body weight and levels of triglycerides, total cholesterol and low‐density lipoprotein cholesterol and abnormally decreased high‐density lipoprotein cholesterol in hyperlipidemic rats, which were significantly raised by a high‐fat diet. Twenty‐seven potential biomarkers were identified within the complex sample matrix of urine. Fourteen biomarkers increased in the hyperlipidemia rats compared with normal rats. Meanwhile, 13 biomarkers decreased. CRCP reversed abnormal changes in biomarkers, including 5‐l‐glutamyl‐taurine, 5‐aminopentanoic acid, cis‐4‐octenedioic acid and 2‐octenedioic acid. These biomarkers show that hyperlipidemia is related to the metabolic pathways of taurine and hypotaurine metabolism, fatty acid biosynthesis, and arginine and proline metabolism. CRCP mainly prevents hyperlipidemia by intervening in these metabolic pathways.

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Section: Discussionmentioning

confidence: 99%

Nontargeted urine metabolomics analysis of the protective and therapeutic effects of Citri Reticulatae Chachiensis Pericarpium on high‐fat feed‐induced hyperlipidemia in rats

Zeng

Huang

Luo

et al. 2020

Biomedical Chromatography

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“…PMFs, a category of important compounds with pharmacological activity, are mainly found in Citrus species of the Rutaceae family [11]. In addition to the formula weight of 222 Da for the basic aglycone structure, the molecular weight will increase by 30 or 16 Da from the addition of a methoxyl or hydroxyl group, respectively, onto the A, B, or C ring.…”

Section: Resultsmentioning

confidence: 99%

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO₂ fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

Zheng

Liu

Chao

et al. 2020

J of Separation Science

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To systematically identify the lipophilic constituents of Citri Reticulatae Pericarpium from different cultivars, supercritical CO2 fluid extraction and ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry were integrated for the component analysis of 18 batches of Citri Reticulatae Pericarpium from 12 cultivars for the first time. A total of 57 components from the supercritical CO2 fluid extracts were demonstrably or tentatively identified by the obtained parent peaks, fragment peaks, and retention times. In total, two flavonoids, six organic acids, nine coumarins, three aldehydes, seven esters, three terpenes, one limonoid, and five other compounds were detected for the first time; notably, coumarin components have not yet been reported in Citri Reticulatae Pericarpium. Furthermore, the extract constituents differed between cultivars. In particular, organic acids were more abundant in Citrus reticulata “Chachi” than in other cultivars, and pterostilbene was exclusively found in Citrus reticulata “Yichangju”. The results showed that a greater variety of compounds in Citri Reticulatae Pericarpium could be extracted by supercritical CO2 fluid extraction and detected by ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry. This study provides a more scientific basis for further analysis of the pharmacological activity and quality of Citri Reticulatae Pericarpium components from different cultivars.

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“…Among Citrus flavonoids, Zeng et al [70] demonstrated that narirutin and didymin were able to inhibit the lipase enzyme with IC 50 values of 58.98 and 67.30 µg/mL, respectively. Previously, Bustanji et al [71] reported the ability of chlorogenic, caffeic, and gallic acids to inhibit the LP and hormone-sensitive lipase (HSL) activities in a dose-dependent manner, but with different potencies.…”

Section: Effect On Carbohydrate-hydrolysing Enzymes and Lipasementioning

confidence: 99%

Citrus × Clementina Hort. Juice Enriched with Its By-Products (Peels and Leaves): Chemical Composition, In Vitro Bioactivity, and Impact of Processing

et al. 2020

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This work investigated a model for the reuse of Citrus × clementina Hort. by-products for the development of a functional drink able to exert antioxidant, hypoglycaemic, and hypolipidemic effects. Juice obtained from fruits collected in three different areas of Calabria (Italy) was analysed. C. × clementina juice from Corigliano Calabro (JF), characterized by the highest content of bioactive compounds and bioactivity, was chosen as a matrix to be enrichment with hydroalcoholic ultrasound-assisted maceration of C. × clementina leaf from Corigliano Calabro (CO2) and ethanol ultrasound-assisted maceration of C. × clementina peel from Cetraro (BC3) extracts at different concentrations. The highest phytochemical content and bioactivities were found in juice enriched with leaf and leaf + peel extracts, with particular reference to antioxidant activity. In order to estimate the effects of pasteurization, 20% (mg/100 mL) enriched juice was subjected to this process. Based on obtained data of bioactivity and sensorial analysis, C. × clementina by-products could be proposed as a promising source of bioactive compounds useful for the formulation of a functional drink for preventing diseases associated with oxidative stress such as type 2 diabetes and obesity.

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Evaluation of anti-lipase activity and bioactive flavonoids in the Citri Reticulatae Pericarpium from different harvest time

Cited by 67 publications

References 23 publications

Nontargeted urine metabolomics analysis of the protective and therapeutic effects of Citri Reticulatae Chachiensis Pericarpium on high‐fat feed‐induced hyperlipidemia in rats

Nontargeted urine metabolomics analysis of the protective and therapeutic effects of Citri Reticulatae Chachiensis Pericarpium on high‐fat feed‐induced hyperlipidemia in rats

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO₂ fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

Citrus × Clementina Hort. Juice Enriched with Its By-Products (Peels and Leaves): Chemical Composition, In Vitro Bioactivity, and Impact of Processing

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Evaluation of anti-lipase activity and bioactive flavonoids in the Citri Reticulatae Pericarpium from different harvest time

Cited by 67 publications

References 23 publications

Nontargeted urine metabolomics analysis of the protective and therapeutic effects of Citri Reticulatae Chachiensis Pericarpium on high‐fat feed‐induced hyperlipidemia in rats

Nontargeted urine metabolomics analysis of the protective and therapeutic effects of Citri Reticulatae Chachiensis Pericarpium on high‐fat feed‐induced hyperlipidemia in rats

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO2 fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

Citrus × Clementina Hort. Juice Enriched with Its By-Products (Peels and Leaves): Chemical Composition, In Vitro Bioactivity, and Impact of Processing

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Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO₂ fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry