Evaluation of antibacterial activity of
            <i>Stenotrophomonas maltophilia</i>
            against
            <i>Ralstonia solanacearum</i>
            under different application conditions

Elhalag, Kamel; Messiha, Nevein A. S.; Emara, H.M.; Abdallah, S. A.

doi:10.1111/jam.13097

Cited by 24 publications

(17 citation statements)

References 65 publications

(105 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pinto, 3–5 leaves/seedling), cultivated in pots under controlled greenhouse conditions (28–30 °C, 75–85% RH, 16 h light/8 h dark) were used for the bioassay test. Soil was infested with equal volumes of the mentioned mixture of virulent strains . The ratio of inoculums/soil was 1:10 (v/w).…”

Section: Methodsmentioning

confidence: 99%

“…Cultivation of resistant cultivars could be a solution, although there are no resistant cultivars available . The use of both Stenotrophomonas maltophilia and salicylic acid as biotic and abiotic elicitors for improving plant immune response proved to have a suppressive effect on the dominant race of R. solanacearum in Egypt under greenhouse conditions .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Potential use of soilborne lytic Podoviridae phage as a biocontrol agent against Ralstonia solanacearum

et al. 2018

View full text Add to dashboard Cite

A new podovirus RsPod1EGY Ralstonia phage (GenBank accession no MG711516) with a specific action against R. solanacearum phylotype IIa, sequevar I (race 3, biovar 2) was isolated from Egyptian soil. The potential efficacy of the isolated phage to be used as biocontrol agent was evaluated in vitro and under greenhouse conditions. The podovirus phage produced a plaque size of 3.0-4.0 mm in diameter and completed its infection cycle in 180 min after infection with a burst size of ∼27 virions per infected cell. On the basis of restriction endonuclease analysis, the genome size of the phage was about 41 kb of double-stranded DNA. In vitro studies showed that RsPod1EGY is stable at higher temperatures (up to 60 °C), and at a wide pH range (5-9). SDS-PAGE analysis indicated the major structural protein to be approximately 32 kDa. Bacteriolytic activity of RsPod1EGY against R. solanacearum was detected at different multiplicity of infection (MOI). RsPod1EGY proved to be effective in reduction and prevention of formation of surface polysaccharides of R. solanacearum, during the exponential growth phase of the latter. Interestingly, RsPod1EGY was effective in suppression of R. solanacearum under greenhouse conditions. All Phage-treated tomato plants showed no wilt symptoms or any latent infection during the experimental period, whereas all untreated plants have wilted by 10 days post-infection. The lytic stability of RsPod1EGY phage at higher temperature as well as its effective suppression of wilting symptoms under greenhouse conditions would contribute to biocontrol the bacterial wilt disease in Egypt under field conditions.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential use of soilborne lytic Podoviridae phage as a biocontrol agent against Ralstonia solanacearum

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Induction of PRs has been found in many plant species belonging to various families, suggestive of a key role for these proteins in adaptation to biotic stress conditions and responsible for the state of SAR (Kombrink and Somssich 1997). Some of PR genes, such as PR1, PR2, and PR5, serve as robust markers for this systemic immune response (Elhalag et al 2016). The expression of pathogenesis-related protein encoded for proteases (PR Fig.…”

Section: Expression Of Pathogenesis-related Gene (Pr-1b) After Scf Apmentioning

confidence: 99%

Induction of potato systemic resistance against the potato virus Y (PVYNTN), using crude filtrates of Streptomyces spp. under greenhouse conditions

Nasr-Eldin

Messiha

Othman

et al. 2019

Egypt J Biol Pest Control

View full text Add to dashboard Cite

The potato virus Y (PVY) is considered one of the most important viruses in terms of both economical and biological impact. Potential of using three different Streptomyces spp. crude filtrates (SCF) to induce of resistance against tuber necrotic strain of potato virus Y (PVY NTN) in four potato cultivars namely, Spunta, Nicola, Selatar, and Diamant, were evaluated. Foliar spraying of Streptomyces netropsis DSM 40093 (SCF7), S. ambofaciens (SCF11) and S. actuosus (SCF20), applied separately on potato cultivated in pots, showed various inhibitory activities against PVY NTN infection in all the potato cultivars. SCF20 was the most effective one, as expressed by (100%) reduction of apical necrosis symptoms on potato cv. Spunta, Nicola, and Diamant leaves under greenhouse conditions. Application was done 7 days before virus inoculation with PVY NTN (DBVI) and 7 days after inoculation (DAVI). The concentration of PVY NTN in treatments was significantly reduced than in the untreated PVY NTN infected plants as detected by DAS-ELISA (expressed by decreasing in OD value). Expression of PR-1b gene was detected 21 (DAVI) in all SCF11-treated cultivars but was not expressed in healthy and infected control plants. Gas chromatography-mass spectrometry (GC-MS) analysis of S. actuosus (SCF20) ethyl acetate extract revealed the majority of fatty acids (FAs) and their derivatives, Citroflex A, Hexanoic acid, anhydride, Hexadecanoic acid, Hexadecanoic acid, ethyl ester, and Diisooctyl phthalate which suggested to act as elicitor molecules for induction of systemic acquired resistance (SAR). Overall, the application of culture filtrates of Streptomyces has the potentiality to be used as inducer against PVY NTN infection.

show abstract

“…First, both genes are related to the defense of tomato against Botrytis cinerea and Alternaria alternata [48]; second, they are related to the protection of grapevine against B. cinerea [49]. On the other hand, Stenotrophomonas species have induced PR proteins in potato, thus decreasing the severity of Ralstonia solanacearum [50].…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

et al. 2020

View full text Add to dashboard Cite

Ancho-type pepper (Capsicum annuum L.) is a crop susceptible to Pythium ultimum, which has already been controlled with synthetic fungicide applications; however, marine antagonist microorganisms could be an alternative source of control. The efficiency in vitro and in vivo of marine bacteria and yeasts was determined against P. ultimum. The inhibition of the radial growth of P. ultimum was quantified in vitro by the bacteria Stenotrophomonas rhizophila KM01 and KM02; Bacillus subtilis RBM01 and RBM02, B. amyloliquefaciens 2RLBF and 3R4CF; and Pseudomonas spp. 2R6BF and 2RE9CF, as well as the yeasts Debaryomyces hansenii 1R11AB, 1R11CB, and LL01 and Cryptococcus laurentii 2R3BF and 2R1CB. The β-1,3-glucanase activity of the marine microorganisms was quantified in the presence of the phytopathogen. The disease index (DI), growth parameters, and colony forming units (CFU) were determined in ancho-type pepper plants inoculated with marine bacteria, yeasts, and P. ultimum. The radial zone of the phytopathogen was inhibited by 80% and 75% by S. rhizophila KM01 and C. laurentii 2R1CB, respectively. D. hansenii LL01 and S. rhizophila KM02 showed the highest activity of β-1,3-glucanase, with 6060 U/mL and 47 U/mL, respectively. B. subtilis RBM02 protected 100% of the plants from the oomycete, and an increase was quantified in all the growth parameters and CFU. The use of these marine bacteria and yeasts are, therefore, an option for P. ultimum biocontrol in ancho-type pepper plants, thereby minimizing the application of synthetic fungicides.Agronomy 2020, 10, 408 2 of 12 by immediate infection makes management of this phytopathogen very difficult [7]. Several Pythium species, including P. aphanidermatum, P. irregular, and P. ultimum, are known to cause damping-off and root rot diseases in pepper [8][9][10]. Although synthetic fungicides have shown promising results in controlling damping-off disease, phytotoxicity and fungicide residues pose serious problems to human and animal health, as well as the environment. In this context, phytosanitary measures and the management of pepper cultivation should include the application of microorganisms as biocontrol agents to minimize the use of synthetic fungicides [11].Bacteria and yeasts have a high capacity to control phytopathogens without causing damage to human and animal health and the environment [12,13]. Several mechanisms have been proposed for the biocontrol of phytopathogens by bacteria and yeasts, including competition for space and nutrients [14,15], lytic enzyme production such as β-1,3-glucanase [16,17], and induction of host resistance [18,19], among others. Both microorganisms have been isolated in different terrestrial ecosystems, mainly in plants, fruits, and soils; the main bacteria used as biocontrol agents are species of Stenotrophomonas, Bacillus, and Pseudomonas, which have already been reported for the control of Fusarium proliferatum in melon [20]; Sclerotium rolfsii [21] and Rhizoctonia solani in wheat [22]; and F. solani in cassava [23], among others. O...

show abstract

Evaluation of antibacterial activity of Stenotrophomonas maltophilia against Ralstonia solanacearum under different application conditions

Abstract: Both SA and peat-moss as an organic carrier enhanced the antibacterial efficiency of the biocontrol agent. Application of Sten. maltophilia is more suitable under alkaline soil conditions.

Cited by 24 publications

References 65 publications

Potential use of soilborne lytic Podoviridae phage as a biocontrol agent against Ralstonia solanacearum

Potential use of soilborne lytic Podoviridae phage as a biocontrol agent against Ralstonia solanacearum

Induction of potato systemic resistance against the potato virus Y (PVYNTN), using crude filtrates of Streptomyces spp. under greenhouse conditions

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

Contact Info

Product

Resources

About