Evaluation of antibacterial and Antibiofilm activity of Synthesized Zinc-Hydroxyapatite Biocomposites from<i>Labeo rohita</i>fish scale waste

Sathiskumar, Swamiappan; Vanaraj, Sekar; Devaraj, Sabarinathan; Preethi, Kathirvel

doi:10.1088/2053-1591/aaae10

Cited by 32 publications

(12 citation statements)

References 54 publications

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“…LB broth was used by adding 2% w/v glucose and seeded into 1 × 1 cm glass slides places in TCP. After 24 h incubation, the glass slides were cleaned using sterilised water for the removal of planktonic cells, after the slides were allowed to stain using 0Á2% w/v Crystal Violet dye solution and biofilm layers were visualized using a light microscope (Kozo Optics, NanJing, China) at 40× magnifications (Stepanović et al 2007;Sathiskumar et al 2018).…”

Section: Anti-biofilm Assaymentioning

confidence: 99%

Characterization and application of rhamnolipid from Pseudomonas plecoglossicida BP03

Devaraj

Vanaraj

Sathiskumar

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: Nowadays chemically synthesized drugs are expensive and create damage to nontarget environment. It creates the demand for low toxic drugs to control and need to achieve immediate results. Microbially derived biosurfactant has been prepared and tried as a drug to various biofilm producers, Anopheles vector and cancer cells. These results confirms the potential ability of rhamnolipid in various toxic environment.

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Section: Anti-biofilm Assaymentioning

confidence: 99%

Characterization and application of rhamnolipid from Pseudomonas plecoglossicida BP03

Devaraj

Vanaraj

Sathiskumar

et al. 2020

Lett Appl Microbiol

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“…LPS induces the bulk release of inflammatory cytokines, which may result in septic shock, systemic inflammatory response syndrome, severe tissue damage, and multiple organ dysfunction (Lamping et al, 1998). With the advent of nanotechnology, the proficiency of HA as an implant in orthopedic and dentistry has been greatly boosted by doping it with chemical elements such as titanium, cobalt, magnesium, zinc, silver, and gold (Lin et al, 2007; Mo et al, 2008; Nirmala et al, 2011; Kramer et al, 2014; Gayathri et al, 2018; Sathiskumar et al, 2018). However, these chemical elements detrimentally interact with blood cells, including erythrocytes, leukocytes, platelets, and macrophages due to the generation of oxidative stress and inflammatory response (Asharani et al, 2010; Chen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells

Nagaraj

Samiappan

2019

Front. Microbiol.

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In the present study, for the first time, biomimetization of hydroxyapatite (HA) with Azadirachta indica (AI) was proposed and established its antioxidant, antibacterial, and anti-inflammatory potential on lipopolysaccharide (LPS). The ethanolic extract of AI was found rich with phenolics and flavonoids, and determined their concentration as 8.98 ± 1.41 mg gallic acid equivalents/g and 5.46 ± 0.84 mg catechin equivalents/g, respectively. The HA was prepared by sol-gel method from calcium nitrate tetrahydrate and orthophosphoric acid, and successfully biomimetization was performed with ethanolic extract of AI. The FTIR analysis settled that as-synthesized HA-AI composite was comprised of both HA and AI. The XRD pattern and Zeta potential revealed that the HA-AI composite was crystalline and negative in charge (−24.0 mV). The average-size distribution, shape, and size of the HA-AI composite was determined as 238.90 d.nm, spherical, and 117.90 nm from size distribution, SEM, and HR-TEM analysis, respectively. The SEM-EDX concluded that the HA-AI composite was comprised of elements of HA as well as AI. The HA-AI composite presented potential antioxidant activity and its EC50 values (dose required to inhibit about half of the radicals) for ABTS and DPPH assays were determined as 115.72 ± 2.33 and 128.51 ± 1.04 μg/ml, respectively. The HA-AI composite showed potent antibacterial activity, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) towards S. aureus (ATCC 700699) and E. coli (ATCC 10536) were correspondingly determined as 266.7 ± 28.87 and 600.0 ± 50.0 μg/ml, and 400.0 ± 86.6 and 816.7 ± 76.38 μg/ml. Most importantly, HA-AI composite presented the potential antiinflammatory response toward lipopolysaccharide (LPS) in RAW 264.7 cells. The dose of 250 μg/ml of HA-AI composite has shown optimum protection against LPS-induced stress (1 μg/ml) by scavenging oxidants and regulating mitochondrial membrane potential (MMP), inflammatory and apoptotic factors. Thus, this study concluded that the impartation of potential biofunctional features to HA from plant sources through biomimetic approach is much beneficial and could find potential application in dentistry and orthopedic.

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“…All the diffracted peaks are describes the formation of ( 200), 512), (432), and (513) planes. 37 Furthermore, the calculated average "a" and "c" values of NHAp (9.422 Ǻ and 6.902 Ǻ) relatively equivalent to the lattice parameters of standard JCPDS 00-009-0432. The d-spacing values were calculated through XRD and indexed (Table S1).…”

Section: Xrd Analysismentioning

confidence: 83%

Effective removal of cationic methylene blue dye using nano‐hydroxyapatite synthesized from fish scale bio‐waste

Sathiskumar

Ganesan

Vanaraj

et al. 2021

Int J Applied Ceramic Tech

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Nano-hydroxyapatite (NHAp) with a novel rod shape was synthesized from an economical and easily accessible Labeo rohita fish scale bio-waste by facile and straightforward alkaline heat treatment method. The purity, functionality, morphology, and surface area of the green synthesized NHAp powder were well-characterized via X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET). The TEM and BET results indicate that the apatite is prepared as a rod-like particle and highly porous with high surface area (112.36 m 2 g −1 ). The NHAp powder was used for the adsorptive removal of cationic dye-methylene blue (MB) from the aqueous samples.Batch experiments were performed to evaluate the mechanism of adsorption and kinetic models. The NHAp achieved an enhanced adsorption efficiency (666.67 mg g −1 ) for the adsorption process. The obtained results perfectly obeyed the pseudo-second-order model, and the Langmuir isotherm exhibited an excellent relationship with the experimental data. Furthermore, thermodynamic studies reveal that the adsorption process was exothermic and spontaneous. Thus, the results proved that Labeo rohita fish scale bio-waste derived NHAp can be inventively utilized as a cost-effective and eco-friendly adsorbent for wastewater treatment.

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Evaluation of antibacterial and Antibiofilm activity of Synthesized Zinc-Hydroxyapatite Biocomposites fromLabeo rohitafish scale waste

Cited by 32 publications

References 54 publications

Characterization and application of rhamnolipid from Pseudomonas plecoglossicida BP03

Characterization and application of rhamnolipid from Pseudomonas plecoglossicida BP03

Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells

Effective removal of cationic methylene blue dye using nano‐hydroxyapatite synthesized from fish scale bio‐waste

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