Evaluation of antimicrobial activity of some plant extracts against antibiotic susceptible and resistant bacterial strains causing wound infection

Atef, Nagwa M.; Shanab, Sanaa M. M.; Negm, Sahar; Abbas, Yasmeen A.

doi:10.1186/s42269-019-0184-9

Cited by 95 publications

(79 citation statements)

References 23 publications

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“…Similar changes in the bacterial cells treated with oregano and thyme essential oils and there by confirmed their antimicrobial power against E. coli O157:H7 [19]. Burt [18] [22,23,24,25,26,27].…”

Section: Scanning Electron Microscopic Observationssupporting

confidence: 58%

Bactericidal Potentiality of Purified Terpenoid Extracts from the Selected Sea Weeds and its Mode of Action

Sumayya

Lubaina²,

Murugan³

2020

J.Trop.Life.Science

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Terpenoids are hydrocarbons involved in a variety of basic functions in plants such as growth, development and other physiological events. Terpenes and their associated molecules safe guard the organisms from pest, pathogen and herbivores. Similarly, therapeutically terpenoids function as antimicrobial agents against bacteria, fungi and viruses. The mechanism of bactericidal activities may be via inhibiting the synthesis of essential molecules like proteins, nucleic acids, cell-wall components, cell membrane derailment, bacterial DNA replication or inhibition of metabolic pathways. The crude methanolic extracts of the seaweeds were subjected to silica gel column chromatographic purification and eluted with different combinations of ethyl acetate: petroleum ether solvent systems. The eluted fractions were further subjected to thin layer chromatography and fractionated by GC-MS. The fractions obtained from Hypnea musciformis revealed the terpenoids such as eicosane, heneicosane, 2-pentadecnone, hexadecanoic acid methyl ester, n-hexadecanoic acid, hexadecanoic acid ethyl ester, heptadecanoic acid methyl ester, 11octadecanoic acid metyl ester, whereas Kappapycus alvarezii displayed hexadecane, eicosane, heptadecane, octadecane, heneicosane, tricosane, hexadecanoic acid, methyl ester and beta amyrin. Similarly, Gracillaria dura revealed hexadecanoic acid methyl ester, n-hexadecanoic acid, 11-octadecanoic acid and phytol. Subsequently, the bactericidal activities of the purified terpenoid extracts from the sea weeds were carried. Initially, the extracts were tested for their in vitro antibacterial activity against six bacterial strains such as three Gram-positive (Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis) and three Gramnegative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa) by disc diffusion method. The results revealed that the purified terpenoid extracts of G. dura exhibited significant bactericidal potentiality against S. mutans as compared to other strains. The zone of inhibition, MIC and MBC values narrate the efficacy of the purified terpenoid extract of the species. Remarkable leaching of metabolites like protein and DNA further substantiates the MIC and MBC results. Scanning electron microscopic observations such as clumping, irregularity of cells and ballooned walls reflect the possible membrane damage accounted in the cells by the terpenoid extracts. Further studies are planned to validate the above data by using molecular tools.

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Section: Scanning Electron Microscopic Observationssupporting

confidence: 58%

Bactericidal Potentiality of Purified Terpenoid Extracts from the Selected Sea Weeds and its Mode of Action

Sumayya

Lubaina²,

Murugan³

2020

J.Trop.Life.Science

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“…Since infection installation is the most common and inevitable impediment to wound healing (Church et al, 2006;Negut et al, 2018), wound dressings loaded with antimicrobial agents have emerged as viable options to reduce wound bacterial colonization and infection, in order to improve the healing process. Gram-positive cocci such as Staphylococcus aureus, and Gram-negative bacilli such as Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia are among the most common pathogenic bacteria isolated from wounds (Pallavali et al, 2017;Atef et al, 2019). It became necessary to investigate the antimicrobial activities of C. planchonii root extract against these bacterial strains.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening and antimicrobial activities of hydroethanolic extracts from leaves and roots of Cochlospermum planchonii (Bixaceae)

Fankibe¹,

Metowogo²,

Kantati³

et al. 2020

J. Pharmacognosy Phytother.

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Cochlospermum planchonii is traditionally used in Togo in treating several diseases, including burn wounds which achievement requires anti-infective and good cicatrisation processes. This study aims to evaluate the antimicrobial properties of hydroethanolic extracts from leaves and roots of C. planchonii and its phytochemical composition. Five (05) bacterial strains were suspended in liquid media with plant extracts at various concentrations and then spread, after 24 h incubation, on solid media. The minimum inhibitory (MIC) and minimal microbicidal (MMC) concentrations were assessed. Phytochemical characterization tests were performed and flavonoids, tannins and total phenol contents were also determined. Hydroethanolic extracts from the leaves and roots of C. planchonii were active on all the germs tested, with MICs ranging from 0.782 to 50 mg/mL and MMCs ranging from 1.563 to 100 mg/mL. The bests activities were recorded with root extract against Staphylococcus aureus: MIC=0.782 mg/mL and MMC =1.563 mg/mL. The MMC/MIC ratios are all equal to 2, indicating that both roots and leaves have bactericidal activities rather than inhibitory activities. Phytochemical tests have demonstrated the presence of metabolites such as flavonoids, tannins, carbohydrates, sterols, triterpenes, saponosides, and an absence of alkaloids. Total phenols, tannins and total flavonoids amounts found were respectively 85.466 ± 0.289; 33.566 ± 0.503; 218.333 ± 0.577 µgRE/mg in root extracts, and 75.833 ± 0.289; 26.533 ± 0,053; 183.666 ± 0.577 µgRE/mg in leaf extracts. Both root and leaf hydroethanolic extracts of C. planchonii could be potential natural antimicrobial remedies.

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“…Assay: Antibacterial activity of the synthesized nanoparticles was investigated by agar well diffusion method 10,11 .…”

Section: Determination Of Antibacterial and Antifungalmentioning

confidence: 99%

Untitled

2020

IJPSR

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The application of nanobiotechnology is an emerging area of nanoscience and nanotechnology. In the present studies, tungsten nanoparticles were synthesized and reduced chemically, and characterrization was done by UV, SEM, TEM, FT-IR, and XRD. The size of nanoparticles was found to be 20 nm. Further, these nanoparticles were teste for various biological applications. In antimicrobial activity, it was observed that potent efficacy was observed against Streptomyces grisveus (12 mm) at 80 µg/ml while in fungus maximum activity was observed against Penicillium funicolusum (24 mm) at the same dose. The antiplatelet activity of nanoparticles was investigated by Prothrombin (PT) and Activated Partial Thromboplastin Time (APTT). In both PT and APTT assay, maximum activity was observed at 40 µg/ml (295 sec and 80 sec, respectively). Cytotoxicity was also studied by MTT assay against various cell lines. Against MCF-7, potent activity was observed at 200 µg/ml, while in 3T3 it was observed at 500 µg/ml. Finally, it was observed that these nanoparticles have potent activity against tuberculosis at 1000 µg/ml. The result showed the nanoparticles are inexpensive and safe without any toxicity and consequently does not have any side effects. INTRODUCTION: In recent years, metal nanoparticles have been prepared with a broad range of applications in various fields, from chemistry to medicine. Mainly, as shapes, sizes, and compositions of metallic nanomaterials are significantly linked to their physical, chemical, and optical properties 1-3 .

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Evaluation of antimicrobial activity of some plant extracts against antibiotic susceptible and resistant bacterial strains causing wound infection

Cited by 95 publications

References 23 publications

Bactericidal Potentiality of Purified Terpenoid Extracts from the Selected Sea Weeds and its Mode of Action

Bactericidal Potentiality of Purified Terpenoid Extracts from the Selected Sea Weeds and its Mode of Action

Phytochemical screening and antimicrobial activities of hydroethanolic extracts from leaves and roots of Cochlospermum planchonii (Bixaceae)

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