Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome

Abstract

BackgroundThe present study was aimed to investigate the antinociceptive and anti-inflammatory activity of the Curcuma zedoaria (family Zingiberaceae) ethanolic rhizome extract in laboratory using both in vitro and in vivo methods so as to justify its traditional use in the above mentioned pathological conditions.MethodsPhytochemical screening was done to find the presence of various secondary metabolites of the plant. In vivo antinociceptive activity was performed employing the hot plate method, acidic acid induced writhing test and formalin induced writhing test on Swiss albino mice at doses of 250 and 500 mg/kg body weight. Anti-inflammatory activity test was done on Long Evans rats at two different doses (250 and 500 mg/kg body weight) by using carrageenan induced paw edema test. Finally in vitro anti-inflammatory test by protein-denaturation method was followed. Data were analyzed by one-way analysis of variance (ANOVA) and Dunnett’s t-test was used as the test of significance. P value <0.05 was considered as the minimum level of significance.ResultsPhytochemical screening revealed presence of tannins, saponins, flavonoids, gums & carbohydrates, steroids, alkaloids, reducing sugars and terpenoids in the extract. In the hot plate method, the extract increased the reaction time of heat sensation significantly to 61.99% and 78.22% at the doses of 250 and 500 mg/kg BW respectively. In acetic acid induced writhing test, the percent inhibition of writhing response by the extract was 48.28% and 54.02% at 250 and 500 mg/kg doses respectively (p < 0.001). The extract also significantly inhibited the licking response in both the early phase (64.49%, p < 0.01) and the late phase (62.37%, p < 0.01) in formalin induced writhing test. The extract significantly (p < 0.05, p < 0.01 and p < 0.001) inhibited carrageenan induced inflammatory response in rats in a dose related manner. In in-vitro anti-inflammatory test, the extract significantly inhibited protein denaturation of 77.15, 64.43, 53.04, 36.78 and 23.70% for doses of 500, 400, 300, 200 and 100 μg/mL respectively.ConclusionsThe results obtained from the tests indicate that the plant might have one or more secondary metabolite(s) having central and peripheral analgesic and anti-inflammatory activity.

Formulation and Evaluation of Novel Antibacterial and Anti-Inflammatory Cream Containing Muntingia Calabura Leaves Extract

“…It is also a defensive attempt by the organism to eradicate the injurious stimuli and initiate the healing process. The cells will undergo activation and release inflammatory mediators at the onset of the inflammation [2]. The inflammatory process involves the release of proinflammatory cytokines, prostaglandins, and the formation of reactive oxygen species.…”

Preliminary Phytochemical and Pharmacological Screenings of Plumbago indica L. and Alpinia conchigera Griff.

“…13 The anti-inflammatory activity of the plant extractives was measured by the means of egg albumin denaturation assay i.e. protein denaturation assay.…”

“…To determine the anti-inflammatory effect of the P. indica and A. conchigera extractives protein denaturation assay was performed according to the method described by Ullah et al (2014). 13 Fifteen clean centrifuge tubes (three for standard acetyl salicylic acid, three for negative control methanol and three for each crude extract) were used. Then, 0.2 ml of 5% egg albumin solution and 2.8 ml phosphate buffer (pH 6.4 ±0.2) were added to all test tubes.…”

Untitled

“…[40] Curcuma zedoaria (family Zingiberaceae) ethanolic rhizome extract shows anti-inflammatory activity. [41] Turmeric is an Ayurvedic medicine practiced for centuries for the treatment of inflammatory disorders including arthritis. It is used as a dietary supplement to treat arthritis treatment [ Table 1].…”