Evaluation of antioxidant, antimicrobial and cytotoxic potential in <i>Artemisia vulgaris</i> L.

The aerial parts of Artemisia vulgaris were subjected for successive extraction in solvents methanol, hexane and chloroform, respectively, and compounds present in extract were analysed by GC-MS analysis 12 diﬀerent compounds were found in chloroform extract in which Germacrene was found most abundantly (33.84%). Chloroform extract showed strong antibacterial activity, which was examined against 6 bacteria using the disc diﬀusion method. The IC50 value of chloroform extract accounted for 1.50 mg/mL. The TPC content of the extract was found to be 87.75 mg gallic acid equivalent/g of dry extract, whereas TFC content was found to be 47.25 mg quercetin equivalent/g of dry extract and exhibited LC50 value of 0.18 mg/mL.

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“…Extracts having LC 50 value less than 1 mg/mL are considered as bioactive in toxicity evaluation by Brine shrimp lethality assay [18,19].…”

Section: Brine Shrimp Lethality Assaymentioning

confidence: 99%

Phytochemical Screening, GC-MS Analysis and Biological Activities of Extracts of Artemisia vulgaris Linn.

et al. 2021

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“…The contents were mixed well and incubated in dark for 30min. Then, the mixture is read for absorbance at 500nm 24,25 .…”

Section: Dpph Assaymentioning

confidence: 99%

β-Sitosterol: An Antibacterial Agent in Aquaculture Management of Vibrio Infections

Ravi¹,

Girish²,

Harshini³

et al. 2020

J Pure Appl Microbiol

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This study aims at investigation of antibacterial property of Parthenium hysterophorous against aquatic bacterial pathogens and to identify the key bioactive compound of the same. Antibacterial activity of the crude extracts confirmed that chloroform extract PHC has strong antibacterial activity against aquatic pathogens V.anguillarum and V.harveyi with 16mm and 15mm ZOI at 1mg/well concentration. The crude extracts were subjected for GC-MS analysis to identify the secondary metabolites. PHC was subjected to silica-gel column chromatography to separate the individual phytochemicals. PHC was separated into 9 fractions, among which Fraction No.2 demonstrated significant antibacterial activity against V.anguillarum and V.harveyi with 19mm and 17mm ZOI at 10µg/well concentration. Fraction No.2 was identified to be β-sitosterol based on mass spectrometry analysis and fragmentation analysis. In-silico protein ligand docking demonstrated that β-sitosterol has highest affinity to inhibit dihydrofolate reductase (DHFR) enzyme with -10.10Kcal/mol binding energy. This prediction was further validated using molecular dynamic simulation for 20ns. Based on these computational analyses, it was proposed that β-sitosterol exhibits antibacterial activity via inhibition of DHFR enzyme. β-sitosterol is a well known nutritionally valuable compound that reduces cholesterol levels in humans. It is also been used as supplement feed to increase the nutritional value of cultured fishes. β-sitosterol has also been proven to have positive effect in growth and reproduction of cultivated fishes. Findings of this study strongly suggest the usage of β-sitosterol in aquaculture, as nutritional supplement and also as disease control agent to prevent and control fish diseases caused by Vibrio species.

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Antioxidant dimension and potential as bacterial growth inhibitors in crude extracts of two Artemisia species (Artemisia indica Willd. and Artemisia maritima L.) from Gilgit-Baltistan region, Pakistan

Rasheed,

Hussain,

Khan

et al. 2024

Vegetos

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Evaluation of antioxidant, antimicrobial and cytotoxic potential in Artemisia vulgaris L.

Cited by 3 publications

References 58 publications

Phytochemical Screening, GC-MS Analysis and Biological Activities of Extracts of Artemisia vulgaris Linn.

Phytochemical Screening, GC-MS Analysis and Biological Activities of Extracts of Artemisia vulgaris Linn.

β-Sitosterol: An Antibacterial Agent in Aquaculture Management of Vibrio Infections

Antioxidant dimension and potential as bacterial growth inhibitors in crude extracts of two Artemisia species (Artemisia indica Willd. and Artemisia maritima L.) from Gilgit-Baltistan region, Pakistan

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