The aerial parts of Artemisia vulgaris were subjected for successive extraction in solvents methanol, hexane and chloroform, respectively, and compounds present in extract were analysed by GC-MS analysis 12 different compounds were found in chloroform extract in which Germacrene was found most abundantly (33.84%). Chloroform extract showed strong antibacterial activity, which was examined against 6 bacteria using the disc diffusion method. The IC50 value of chloroform extract accounted for 1.50 mg/mL. The TPC content of the extract was found to be 87.75 mg gallic acid equivalent/g of dry extract, whereas TFC content was found to be 47.25 mg quercetin equivalent/g of dry extract and exhibited LC50 value of 0.18 mg/mL.

Fruit part of Trachyspermum ammi (L.) Sprague was subjected to extraction of essential oil by hydrodistillation in Clevenger apparatus. So collected essential oil was analyzed by GC-MS for its composition and exhibited the presence of 10 different compounds. The most abundant were γ-terpinene (53.81%) and thymol (29.40%). Antibacterial activity was performed against six bacterial species and Staphylococcus aureus, Enterobacter cloaceae and Bacillus subtilis were the most susceptible to the essential oil showing zone of inhibition (ZOI) 1.4, 1.5 and 1.4 cm respectively. The IC50 value of the oil against DPPH was found to be 0.94 mg/mL. The LC50 value of essential oil of T. ammi against brine shrimp was found 26.2 μg/mL.

The powdered plant materials of Acorus calamus were subjected to successive extraction using the cold percolation method with methanol, hexane, and chloroform solvent. Qualitative phytochemical analysis of methanol, hexane, and chloroform extracts showed the presence of alkaloids, saponins, glycosides, sterols, triterpenoids, and carbohydrates. Five different major compounds were identified by GC-MS analysis of the chloroform extract, with isoprothiolane (83.11%) being the most prevalent. The total phenolic content in the chloroform extract was calculated at 17.39 mg Gallic acid equivalent /g and the total flavonoid content was 3.37 mg quercetin equivalent/g of dry extract. The IC50 value of chloroform extract was found to be 576.19 μg/mL, and the LC50 value was found to be 66.21 μg/mL. Antibacterial activity was shown in Staphylococcus aureus in chloroform extract with a ZOI of 7 mm.

The powdered leaf of Ageratina adenophora was subjected to extraction using a cold percolation method with methanol. After suspending the crude methanol extract in 1% HCl and neutralizing with NH4OH, the obtained solution was extracted with hexane, chloroform, and ethyl acetate solvent, respectively. Qualitative phytochemical analysis of methanol, hexane, chloroform, and ethyl acetate extracts of A. adenophora plant showed the presence of alkaloids, flavonoids, phenols, steroids, quinones, saponins, tannins, cardiac glycosides, carbohydrate, terpenoids, proteins, and amino acids. GC-MS analysis of chloroform extract showed 10 different major compounds in which α-Muurolol (24.33%) was found most abundant. The IC50 value of chloroform extract was found to be 1460 µg/mL from DPPH scavenging antioxidant assay, and the LC50 value was found to be 174.78 µg/mL from the brine shrimp lethality assay. Antibacterial activity was shown the highest against Escherichia coli and Proteus vulgaris with ZOI of 12 mm on each in chloroform extract. It was determined that 89.75 mg of gallic acid equivalent/g of dry extract accounted up the total phenolic content and 49.25 mg of quercetin equivalent/g of dry extract was observed to be the total flavonoid content in the chloroform extract.